Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 17th August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Extraction of pPS16003, pPS16004 and GFP1-9_PSB1C3 (clones 2 and 3) 1.1.1.2 Purification of gBlocks 2, 3 and 4 1.1.1.3 Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM 2 = Wednesday 17th August Lab work Visualization Extraction of pPS16003, pPS16004 and GFP1-9_PSB1C3 (clones 2 and 3) By Charlène Clones 2 and 3 of GFP1-9_PSB1C3, and pPS16003, pPS16004 were extracted with the Plasmid MiniPrep kit. The extracts were put to migrated on a 0.8%agarose gel with BET. Purification of gBlocks 2, 3 and 4 By Terrence The purification was carried out following the usual protocol. Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM By Léa and Naiane The cloning was carried out using a new protocol which uses pJET as cloning vector. A heat shock transformation was made on the cloning samples using the following protocol =
By Charlène
Clones 2 and 3 of GFP1-9_PSB1C3, and pPS16003, pPS16004 were extracted with the Plasmid MiniPrep kit.
The extracts were put to migrated on a 0.8%agarose gel with BET.
By Terrence
The purification was carried out following the usual protocol.
By Léa and Naiane
The cloning was carried out using a new protocol which uses pJET as cloning vector.
A heat shock transformation was made on the cloning samples using the following protocol
