Team:BroadRun-Baltimore/Parts

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Parts

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New Parts Submitted to the Registry in 2016

Part Name	Part Number
Penicillium alpha amylase w/ no promoter	BBa_K2185003
B.cereus beta amylase w/ no promoter	BBa_K2185005
S.ferax alpha amylase w/ cyc promoter	BBa_K2185007

Composite Part 1: BBa_K2185003

Promoterless Kozak sequence (BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Alpha amylase coding sequence from the fungus Penicillium ADH1 Terminator (Part BBa_K392003)

Composite Part 2: BBa_K2185005

Promoterless Kozak sequence (Part BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Beta amylase coding sequence from Bacillus cereus ADH1 Terminator (Part BBa_K392003)

Composite Part 3: BBa_K2185007

pCyc medium promoter (BBa_I766555) Kozak sequence (Part BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Alpha amylase coding sequence from Saprolegnia ferax ADH1 Terminator (Part BBa_K392003)

Description of Basic Parts:

ADH1 Terminator - This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the DNA sequence.

Kozak Sequence - This part initiates translation from eukaryotic mRNA and is placed between a promoter and coding sequence to facilitate translation.

pCyc medium promoter - This is a medium expression level constitutive promoter. It directs the cell to transcribe the DNA sequence constantly.

Mating Factor Alpha Secretion Sequence - This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein.

Penicillium Alpha Amylase - Obtained from the fungus Penicillium, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.

S.ferax Alpha Amylase - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.

B.cereus Beta Amylase - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses beta bonds of large, beta-linked polysaccharides, such as cellulose and some starches, thus degrading the polysaccharides into monosaccharides and disaccharides.

In addition to the 3 new parts submitted this year, we also improved a previous part, from last year. To see how we improved the characterization of this part, please see here.