Team:IIT-Madras/Parts
Composite Parts
We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. These parts include Promoter-RBS composite parts, dual fluorescence reporter devices, RIBOS devices and sgRNA parts. To know more about the characterization and functionality of these parts and their future applications.
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Part Collection
We have submitted one part collection for efficient characterization of devices e.g. noise & strength. This part collection contains 12 composite parts. To know more about the characterization and functionality of these parts and their future applications.
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Size Characterization
Following is the gel picture of our constructs, P represents plasmid DNA and D represents plasmid DNA digested with EcoRI-HF and PstI:
Table of important parts
| Part Name | Part Number |
|---|---|
| pL-RBS 1 | BBa_K1956001 |
| pL-RBS 2 | BBa_K1956002 |
| pL-RBS 3 | BBa_K1956003 |
| pL-RBS 4 | BBa_K1956004 |
| pL-RIBOSOFF | BBa_K1956005 |
| pL-gRNA1_CamR | BBa_K1956006 |
| pL-gRNA2_CamR | BBa_K1956007 |
| pL-gRNA1_mCherry | BBa_K1956008 |
| pL-gRNA2_mCherry | BBa_K1956009 |
| pL-gRNA_KanR | BBa_K1956010 |
| pL-gRNA1_CamR-DT | BBa_K1956011 |
| pL-gRNA1_mCherry-DT | BBa_K1956012 |
| pL-gRNA_KanR-DT | BBa_K1956013 |
| Noise in Device Kit 3 | BBa_K1956014 |
| Noise in Device kit 4 | BBa_K1956015 |
| Expression vector for efficient Characterization | BBa_K1956016 |
| Noise in Device kit 5 | BBa_K1956017 |
| Noise in Device kit 6 | BBa_K1956018 |
| RIBOSON for mCherry RNA with K1956016 | BBa_K1956019 |
| RIBOSOFF 1 for lacI RNA with K1956016 | BBa_K1956020 |
| RIBOSOFF2_lacIlacI-K1956016 | BBa_K1956021 |
| Noise in Device Kit 1 | BBa_K1956022 |
| Noise in Device Kit 2 | BBa_K1956023 |
| LacI expression device | BBa_K1956034 |
