Team:Northwestern/06 23

Notebook

Thursday, June 23rd

Results:

Kelly’s competent cells performed well; ours did not. The iGEM DNA was very dilute. We have no data from the negative control, and we’re using results from Kelly’s cells.

Figure 1: Transformation plates

Figure 1(cont): Transformation plates

Tasks:

Sara

  • Background research on alternate membrane proteins
  • Looked for parts

Tasfia

  • Researched on history of getting ClyA into membranes in vivo
  • Learned how to use Genome compiler

Michelle

  • CSS research for the website
  • Background research
  • Worked on constructs

Tyler

  • Researched ClyA’s orientation and how to reduce toxicity
  • Presentation for tomorrow
  • ClyA-His6 part

Sam

  • Designed the Tat-his6 part

Jordan

  • Picked colonies from the transformation from the night before
  • Innoculated more cells

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