Team:TU Delft/Parts

iGEM TU Delft

Parts

For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite. The different basic parts comprising the composite parts are documented seperately, although they do not exist induvidually. In the case of a constitutive expression, the promoter is included in the biobrick itself. For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do not contain a promoter. Additionally, using PCR rather than synthesis, we constructed a small collection of GFP expression devices with different promoter strengths, based on an existing biobrick. Thereby improving both the function and characterization of the original part

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Composite Parts

For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite.

Name Type Description Designer Length
BBa_K1890000 Composite Silicatein gene with strong RBS Lycka Kamoen, Maria Vazquez 686
BBa_K1890001 Composite Silicatein gene, fused to transmembrane domain of INP, with strong RBS Lycka Kamoen, Maria Vazquez 1442
BBa_K1890002 Composite Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS Lycka Kamoen, Maria Vazquez 1481
BBa_K1890010 Composite mCerulean with strong consitutive promoter and RBS Lycka Kamoen, Maria Vazquez 784
BBa_K1890011 Composite mVenus with strong consitutive promoter and RBS Lycka Kamoen, Maria Vazquez 784
BBa_K1890020 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890021 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890022 Generator GFP with medium constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890023 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890024 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890030 Composite BolA gene with RBS and terminator Lycka Kamoen, Maria Vazquez 508
BBa_K1890031 Composite BolA gene with strong promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 519

Basic Parts

The different basic parts comprising the synthesized parts we used are documented here. Please note that these parts do not exist individually.

Name Type Description Designer Length
BBa_K1890050 Coding Silicatein from Suberites domuncula Lycka Kamoen, Maria Vazquez 660
BBa_K1890051 Coding Silicatein from Tethya aurantium Lycka Kamoen, Maria Vazquez 993
BBa_K1890052 Protein_Domain OmpA transmembrane domain Lycka Kamoen, Maria Vazquez 462
BBa_K1890053 Protein_Domain INP transmembrane domain Lycka Kamoen, Maria Vazquez 732
BBa_K1890054 Coding mCerulean Lycka Kamoen, Maria Vazquez 720
BBa_K1890055 Coding mVenus Lycka Kamoen, Maria Vazquez 720
BBa_K1890056 Coding BolA Lycka Kamoen, Maria Vazquez 321
BBa_K1890058 DNA Linker Lycka Kamoen, Maria Vazquez 24

Part Collection

We provided a small collection of GFP expression devices with different promoter strengths, which we used to study the effect of different levels of constitutive expression. These parts are ready-to-go expression units for green fluorescence, which can be useful for future teams. By constructing these parts, we improved both the function and the characterization of the original part BBa_E0840.

Name Type Description Designer Length
BBa_K1890020 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890021 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890022 Generator GFP with medium constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890023 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890024 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913