Team:TU Delft/Parts
Parts
For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite. The different basic parts comprising the composite parts are documented seperately, although they do not exist induvidually. In the case of a constitutive expression, the promoter is included in the biobrick itself. For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do not contain a promoter. Additionally, using PCR rather than synthesis, we constructed a small collection of GFP expression devices with different promoter strengths, based on an existing biobrick. Thereby improving both the function and characterization of the original part
<groupparts class="parts">iGEM2016 TU_Delft</groupparts>
Composite Parts
For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1890000 | Composite | Silicatein gene with strong RBS | Lycka Kamoen, Maria Vazquez | 686 |
| BBa_K1890001 | Composite | Silicatein gene, fused to transmembrane domain of INP, with strong RBS | Lycka Kamoen, Maria Vazquez | 1442 |
| BBa_K1890002 | Composite | Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS | Lycka Kamoen, Maria Vazquez | 1481 |
| BBa_K1890010 | Composite | mCerulean with strong consitutive promoter and RBS | Lycka Kamoen, Maria Vazquez | 784 |
| BBa_K1890011 | Composite | mVenus with strong consitutive promoter and RBS | Lycka Kamoen, Maria Vazquez | 784 |
| BBa_K1890020 | Generator | GFP with strong constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890021 | Generator | GFP with strong constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890022 | Generator | GFP with medium constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890023 | Generator | GFP with weak constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890024 | Generator | GFP with weak constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890030 | Composite | BolA gene with RBS and terminator | Lycka Kamoen, Maria Vazquez | 508 |
| BBa_K1890031 | Composite | BolA gene with strong promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 519 |
Basic Parts
The different basic parts comprising the synthesized parts we used are documented here. Please note that these parts do not exist individually.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1890050 | Coding | Silicatein from Suberites domuncula | Lycka Kamoen, Maria Vazquez | 660 |
| BBa_K1890051 | Coding | Silicatein from Tethya aurantium | Lycka Kamoen, Maria Vazquez | 993 |
| BBa_K1890052 | Protein_Domain | OmpA transmembrane domain | Lycka Kamoen, Maria Vazquez | 462 |
| BBa_K1890053 | Protein_Domain | INP transmembrane domain | Lycka Kamoen, Maria Vazquez | 732 |
| BBa_K1890054 | Coding | mCerulean | Lycka Kamoen, Maria Vazquez | 720 |
| BBa_K1890055 | Coding | mVenus | Lycka Kamoen, Maria Vazquez | 720 |
| BBa_K1890056 | Coding | BolA | Lycka Kamoen, Maria Vazquez | 321 |
| BBa_K1890058 | DNA | Linker | Lycka Kamoen, Maria Vazquez | 24 |
Part Collection
We provided a small collection of GFP expression devices with different promoter strengths, which we used to study the effect of different levels of constitutive expression. These parts are ready-to-go expression units for green fluorescence, which can be useful for future teams. By constructing these parts, we improved both the function and the characterization of the original part BBa_E0840.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1890020 | Generator | GFP with strong constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890021 | Generator | GFP with strong constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890022 | Generator | GFP with medium constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890023 | Generator | GFP with weak constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890024 | Generator | GFP with weak constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
