Difference between revisions of "Team:TU Delft/Parts"

Line 74: Line 74:
 
                         <h2 class="title-style-1">Composite Parts<span class="title-under"></span></h2>
 
                         <h2 class="title-style-1">Composite Parts<span class="title-under"></span></h2>
 
                         <div class="col-md-10 col-md-offset-1"
 
                         <div class="col-md-10 col-md-offset-1"
 +
                            <p>For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to
 +
                            generate our required parts in a fast and efficient way. All of the parts were designed containing the coding
 +
                            sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite.
 +
                            The different units comprising the composite parts are documented seperately in the "basic parts" section.
 +
                            These basic parts do therefore not exist induvidually.</p>
 +
                            <p>In the case of a constitutive expression, the promoter is already present in the biobrick itself.
 +
                            For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter
 +
                            and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do
 +
                            not contain a promoter.</p>
 
                             <table>
 
                             <table>
  
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                                             <td>Lycka Kamoen, Maria Vazquez</td>
 
                                             <td>Lycka Kamoen, Maria Vazquez</td>
 
                                             <td align="right">784</td>
 
                                             <td align="right">784</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890020">BBa_K1890020</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890021">BBa_K1890021</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890022">BBa_K1890022</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with medium constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890023">BBa_K1890023</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890024">BBa_K1890024</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 
                                         </tr>
 
                                         </tr>
 
                                         <tr>
 
                                         <tr>
Line 129: Line 173:
 
                                             <td>Lycka Kamoen, Maria Vazquez</td>
 
                                             <td>Lycka Kamoen, Maria Vazquez</td>
 
                                             <td align="right">508</td>
 
                                             <td align="right">508</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890031">BBa_K1890031</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>BolA gene with strong promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">519</td>
 
                                         </tr>
 
                                         </tr>
 
                                     </tbody>
 
                                     </tbody>

Revision as of 06:49, 16 October 2016

iGEM TU Delft

Parts

All Parts

                               <groupparts class="parts">iGEM2016 TU_Delft</groupparts>

Composite Parts

For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite. The different units comprising the composite parts are documented seperately in the "basic parts" section. These basic parts do therefore not exist induvidually.

In the case of a constitutive expression, the promoter is already present in the biobrick itself. For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do not contain a promoter.

Name Type Description Designer Length
BBa_K1890000 Composite Silicatein gene with strong RBS Lycka Kamoen, Maria Vazquez 686
BBa_K1890001 Composite Silicatein gene, fused to transmembrane domain of INP, with strong RBS Lycka Kamoen, Maria Vazquez 1442
BBa_K1890002 Composite Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS Lycka Kamoen, Maria Vazquez 1481
BBa_K1890010 Composite mCerulean with strong consitutive promoter and RBS Lycka Kamoen, Maria Vazquez 784
BBa_K1890011 Composite mVenus with strong consitutive promoter and RBS Lycka Kamoen, Maria Vazquez 784
BBa_K1890020 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890021 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890022 Generator GFP with medium constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890023 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890024 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890030 Composite BolA gene with RBS and terminator Lycka Kamoen, Maria Vazquez 508
BBa_K1890031 Composite BolA gene with strong promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 519

Basic Parts

Part Collection

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