Difference between revisions of "Team:TU Delft/Parts"
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<h1 class="page-header">Parts<span class="title-under"></span></h1> | <h1 class="page-header">Parts<span class="title-under"></span></h1> | ||
| + | <p>For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to | ||
| + | generate our required parts in a fast and efficient way. All of the parts were designed containing the coding | ||
| + | sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are <a href="#composite"><b>composite</b></a>. | ||
| + | The different <a href="#basic"><b>basic</b></a> parts comprising the composite parts are documented seperately, although they do not exist induvidually.</p> | ||
| + | <p>In the case of a constitutive expression, the promoter is included in the biobrick itself. | ||
| + | For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter | ||
| + | and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do | ||
| + | not contain a promoter.</p> | ||
| + | <p>Additionally, using PCR rather than synthesis, we constructed a small | ||
| + | <a href="#collection"><b>collection</b></a> of GFP expression devices, based on an existing biobrick.</p> | ||
| + | |||
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Revision as of 07:45, 16 October 2016
Parts
For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite. The different basic parts comprising the composite parts are documented seperately, although they do not exist induvidually.
In the case of a constitutive expression, the promoter is included in the biobrick itself. For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do not contain a promoter.
Additionally, using PCR rather than synthesis, we constructed a small collection of GFP expression devices, based on an existing biobrick.
All Parts
<groupparts class="parts">iGEM2016 TU_Delft</groupparts>
Composite Parts
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1890000 | Composite | Silicatein gene with strong RBS | Lycka Kamoen, Maria Vazquez | 686 |
| BBa_K1890001 | Composite | Silicatein gene, fused to transmembrane domain of INP, with strong RBS | Lycka Kamoen, Maria Vazquez | 1442 |
| BBa_K1890002 | Composite | Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS | Lycka Kamoen, Maria Vazquez | 1481 |
| BBa_K1890010 | Composite | mCerulean with strong consitutive promoter and RBS | Lycka Kamoen, Maria Vazquez | 784 |
| BBa_K1890011 | Composite | mVenus with strong consitutive promoter and RBS | Lycka Kamoen, Maria Vazquez | 784 |
| BBa_K1890020 | Generator | GFP with strong constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890021 | Generator | GFP with strong constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890022 | Generator | GFP with medium constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890023 | Generator | GFP with weak constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890024 | Generator | GFP with weak constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890030 | Composite | BolA gene with RBS and terminator | Lycka Kamoen, Maria Vazquez | 508 |
| BBa_K1890031 | Composite | BolA gene with strong promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 519 |
Basic Parts
Part Collection
We provided a small collection of GFP expression divices with different promoter strengths, which we used to study the effect of constitutive expression. These parts are ready-to-go expression units for green fluorescence, which can be usefull for future teams.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1890020 | Generator | GFP with strong constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890021 | Generator | GFP with strong constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890022 | Generator | GFP with medium constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890023 | Generator | GFP with weak constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
| BBa_K1890024 | Generator | GFP with weak constitutive promoter, RBS and terminator | Lycka Kamoen, Maria Vazquez | 913 |
