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+ | .home_blue{ | ||
+ | color:#00a0ea; | ||
+ | } | ||
</style> | </style> | ||
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</div> | </div> | ||
<div class="container_1_content"> | <div class="container_1_content"> | ||
− | <h6>Solar energy is the most abundant | + | <h6>Solar energy is the most abundant form of energy on earth, but is extremely hard to use. Thus scientists have been trying different methods to convert solar energy to chemical energy. To transfer light to allow sustainable production of chemicals motivates the development of <span class="home_blue">artificial photosynthetic systems</span>. Two main problems to be solved in this system are the electron transport media and enzymes to harvest excited electrons. The current transportation method either requires expensive semiconductor nanoparticles or special species which are inconvenient for gene operation. </h6> |
<p> </p> | <p> </p> | ||
− | <h6> | + | <h6>What’s more, for downstream enzymes that harvest electrons, including hydrogenase and nitrogenase, we found that they are <span class="home_blue">generally oxygen-intolerant</span>. So we also want to build an oxygen-isolation system.</h6> |
<p> </p> | <p> </p> | ||
− | <h6> | + | <h6>This year we managed to solve all the problems mentioned above once and forever in synthetic biology. Our project proposes a new method to construct an <span class="home_blue">artificial photosynthetic system</span> in model organism <em>E.coli</em> with the help of metal-binding proteins and surface display machinery. In addition, using silicon encapsulation, we <span class="home_blue">overcome oxygen intolerance</span> barriers of enzymes with assistance from polymer materials. </h6> |
<p> </p> | <p> </p> | ||
− | <h6> | + | <h6>To test the efficiency of our artificial photosynthetic system and silicon encapsulation method, we use hydrogen production as an example. We successfully achieve aerobic hydrogen production using <em>E.coli</em> hydrogenase 1 under normal conditions. What is really exciting as that our new light-driven system can also be extended to more model species including yeast and bacillus, so do the encapsulation system!</h6> |
− | + | ||
<p> </p> | <p> </p> | ||
− | <h6> | + | <h6>The flow chart below shows how our <strong>artificial photosynthetic system</strong> and <strong>silicon encapsulation system</strong> works corporately to achieve hydrogen production!</h6> |
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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} | } | ||
− | #menu_project .page h2, #menu_project .page | + | #menu_project .page h2 |
+ | { | ||
+ | font-size:48px; | ||
+ | color:#00a0ea; | ||
+ | text-align:center; | ||
+ | font-family: "Comic Sans MS", cursive; | ||
+ | padding: 0; | ||
+ | margin: 0; | ||
+ | } | ||
+ | |||
+ | #menu_project .page h6 | ||
{ | { | ||
+ | font-size: 36px; | ||
+ | line-height:50px; | ||
+ | color:#00a0ea; | ||
+ | text-align:center; | ||
+ | font-family: "Comic Sans MS", cursive; | ||
padding: 0; | padding: 0; | ||
margin: 0; | margin: 0; | ||
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{ | { | ||
padding:30px; | padding:30px; | ||
− | height: | + | height: 600px; |
position: relative; | position: relative; | ||
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#menu_project #page1 ul li:first-child | #menu_project #page1 ul li:first-child | ||
{ | { | ||
− | top: | + | top: 200px; |
left: 600px; | left: 600px; | ||
width: 600px; | width: 600px; | ||
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position: absolute; | position: absolute; | ||
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left: 0px; | left: 0px; | ||
width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(T--Nanjing-China-- | + | background-image:url(https://static.igem.org/mediawiki/2016/a/a9/T--Nanjing-China--home_project_1_2.png); |
background-size:contain; | background-size:contain; | ||
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{ | { | ||
position: absolute; | position: absolute; | ||
− | top: | + | top: 160px; |
left: 255px; | left: 255px; | ||
− | width: | + | width: 300px; |
height: 300px; | height: 300px; | ||
− | background-image:url(https://static.igem.org/mediawiki/2016/ | + | background-image:url(https://static.igem.org/mediawiki/2016/1/1f/T--Nanjing-China--home_project_1_1.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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<div class="page" id="page1"> | <div class="page" id="page1"> | ||
− | <div data-anchor-target="#page1" data-- | + | <div data-anchor-target="#page1" data--450-top="opacity: 1" data--650-top="opacity: 0"> |
− | <header data-anchor-target="#page1" data- | + | <header data-anchor-target="#page1" data-250-top="opacity: 1" data-550-top="opacity: 0"> |
− | <h2> | + | <h2 style="padding-top:20px;padding-bottom:20px;">Subcloning</h2> |
− | + | ||
</header> | </header> | ||
− | + | <div id="yeast-red1" data-anchor-target="#page1 header h2" data-150-top="left: 250px; opacity: 1" data-550-top="left: 400px; opacity: 0"></div> | |
− | + | <div id="yeast-blue1" data-anchor-target="#page1 header h2" data-150-top="left: 263px; opacity: 1" data-550-top="left: 100px; opacity: 0"></div> | |
− | <div id="yeast-blue1" data-anchor-target="#page1 header h2" data- | + | |
− | <div data-anchor-target="#page1 header h2" data- | + | <div data-anchor-target="#page1 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0"> |
<ul> | <ul> | ||
<li> | <li> | ||
− | < | + | <h6>1. Plasmids encoding the OmpA-PbrR fused protein and Hya ABCDEF are co-transfered in to E.coli strain BL21(DE3).</h6> |
</li> | </li> | ||
</ul> | </ul> | ||
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<style> | <style> | ||
− | #menu_project # | + | #menu_project #page4 |
{ | { | ||
padding:30px; | padding:30px; | ||
− | height: | + | height: 600px; |
position: relative; | position: relative; | ||
} | } | ||
− | #menu_project # | + | #menu_project #page4 h2 |
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{ | { | ||
padding: 0 55px; | padding: 0 55px; | ||
} | } | ||
− | #menu_project # | + | #menu_project #page4 header p |
{ | { | ||
font-size: 24px; | font-size: 24px; | ||
} | } | ||
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width: 600px; | width: 600px; | ||
} | } | ||
− | #menu_project # | + | #menu_project #page4 #yeast-red4 |
{ | { | ||
position: absolute; | position: absolute; | ||
− | top: | + | top: 163px; |
left: 0px; | left: 0px; | ||
width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(T--Nanjing-China-- | + | background-image:url(https://static.igem.org/mediawiki/2016/4/41/T--Nanjing-China--home_project_2_1.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
} | } | ||
− | #menu_project # | + | #menu_project #page4 #yeast-blue4 |
{ | { | ||
position: absolute; | position: absolute; | ||
− | top: | + | top: 160px; |
left: 255px; | left: 255px; | ||
width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(https://static.igem.org/mediawiki/2016/ | + | background-image:url(https://static.igem.org/mediawiki/2016/8/8e/T--Nanjing-China--home_project_2_2_2.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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</style> | </style> | ||
− | <div class="page" id=" | + | <div class="page" id="page4"> |
− | <div data-anchor-target="# | + | <div data-anchor-target="#page4" data--450-top="opacity: 1" data--650-top="opacity: 0"> |
− | <header data-anchor-target="# | + | <header data-anchor-target="#page4" data-250-top="opacity: 1" data-550-top="opacity: 0"> |
− | <h2> | + | <h2 style="padding-top:20px;padding-bottom:20px;">PS System Construction</h2> |
− | + | ||
</header> | </header> | ||
− | + | <div id="yeast-red4" data-anchor-target="#page4 header h2" data-150-top="left: 231px; opacity: 1" data-550-top="left: 100px; opacity: 0"></div> | |
− | + | ||
− | <div id="yeast- | + | <div id="yeast-blue4" data-anchor-target="#page4 header h2" data-150-top="left: 270px; opacity: 1" data-550-top="left: 400px; opacity: 0"></div> |
− | <div data-anchor-target="# | + | <div data-anchor-target="#page4 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0"> |
<ul> | <ul> | ||
<li> | <li> | ||
− | < | + | <h6>2. Induced expression of OmpA-PbrR and hydrogenase in order to construct our articifial photosynthetic system.</h6> |
</li> | </li> | ||
</ul> | </ul> | ||
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<style> | <style> | ||
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{ | { | ||
padding:30px; | padding:30px; | ||
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position: relative; | position: relative; | ||
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− | #menu_project # | + | #menu_project #page2 h2 |
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− | #menu_project # | + | #menu_project #page2 header p |
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font-size: 24px; | font-size: 24px; | ||
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left: 600px; | left: 600px; | ||
width: 600px; | width: 600px; | ||
} | } | ||
− | + | #menu_project #page2 #yeast-red2 | |
− | #menu_project # | + | |
{ | { | ||
position: absolute; | position: absolute; | ||
− | top: | + | top: 160px; |
left: 0px; | left: 0px; | ||
width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(https://static.igem.org/mediawiki/2016/ | + | background-image:url(https://static.igem.org/mediawiki/2016/8/84/T--Nanjing-China--home_project_3_1.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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− | #menu_project # | + | #menu_project #page2 #yeast-blue2 |
{ | { | ||
position: absolute; | position: absolute; | ||
− | top: | + | top: 160px; |
left: 255px; | left: 255px; | ||
width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(https://static.igem.org/mediawiki/2016/ | + | background-image:url(https://static.igem.org/mediawiki/2016/5/54/T--Nanjing-China--home_project_3_2.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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</style> | </style> | ||
− | <div class="page" id=" | + | <div class="page" id="page2"> |
− | <div data-anchor-target="# | + | <div data-anchor-target="#page2" data--450-top="opacity: 1" data--650-top="opacity: 0"> |
− | <header data-anchor-target="# | + | <header data-anchor-target="#page2" data-250-top="opacity: 1" data-550-top="opacity: 0"> |
− | <h2> | + | <h2 style="padding-top:20px;padding-bottom:20px;">In Situ Particles</h2> |
− | + | ||
</header> | </header> | ||
− | <div id="yeast- | + | <div id="yeast-red2" data-anchor-target="#page2 header h2" data-150-top="left: 242px; opacity: 1" data-550-top="left: 100px; opacity: 0"></div> |
+ | <div id="yeast-blue2" data-anchor-target="#page2 header h2" data-150-top="left: 250px; opacity: 1" data-550-top="left: 400px; opacity: 0"></div> | ||
− | <div | + | <div data-anchor-target="#page2 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0"> |
− | + | ||
− | + | ||
<ul> | <ul> | ||
<li> | <li> | ||
− | < | + | <h6>3. The light driven system is constructed by in situ formed CdS particles on the outer membrane of <em>E.coli.</em></h6> |
</li> | </li> | ||
</ul> | </ul> | ||
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</section> | </section> | ||
− | + | <section> | |
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{ | { | ||
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position: relative; | position: relative; | ||
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width: 600px; | width: 600px; | ||
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− | #menu_project # | + | #menu_project #page5 #yeast-red5 |
{ | { | ||
position: absolute; | position: absolute; | ||
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width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(T--Nanjing-China-- | + | background-image:url(https://static.igem.org/mediawiki/2016/b/bf/T--Nanjing-China--home_project_4_1.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
} | } | ||
− | #menu_project # | + | #menu_project #page5 #yeast-blue5 |
{ | { | ||
position: absolute; | position: absolute; | ||
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left: 255px; | left: 255px; | ||
width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(T--Nanjing-China-- | + | background-image:url(https://static.igem.org/mediawiki/2016/5/5c/T--Nanjing-China--home_project_4_2.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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</style> | </style> | ||
− | <div class="page" id=" | + | <div class="page" id="page5"> |
− | <div data-anchor-target="# | + | <div data-anchor-target="#page5" data--450-top="opacity: 1" data--650-top="opacity: 0"> |
− | <header data-anchor-target="# | + | <header data-anchor-target="#page5" data-250-top="opacity: 1" data-550-top="opacity: 0"> |
− | <h2> | + | <h2 style="padding-top:20px;padding-bottom:20px;">LbL Assembly</h2> |
− | + | ||
</header> | </header> | ||
− | <div id="yeast- | + | <div id="yeast-red5" data-anchor-target="#page5 header h2" data-150-top="left: 237px; opacity: 1" data-550-top="left: 100px; opacity: 0"></div> |
− | <div id="yeast- | + | <div id="yeast-blue5" data-anchor-target="#page5 header h2" data-150-top="left: 265px; opacity: 1" data-550-top="left: 400px; opacity: 0"></div> |
− | <div data-anchor-target="# | + | <div data-anchor-target="#page5 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0"> |
<ul> | <ul> | ||
<li> | <li> | ||
− | < | + | <h6>4. Bacteria is warpped into multi-layered polymer materials.</h6> |
</li> | </li> | ||
</ul> | </ul> | ||
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<style> | <style> | ||
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{ | { | ||
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position: relative; | position: relative; | ||
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− | #menu_project # | + | #menu_project #page3 header p |
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left: 600px; | left: 600px; | ||
width: 600px; | width: 600px; | ||
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− | #menu_project # | + | |
+ | #menu_project #page3 #yeast-red3 | ||
{ | { | ||
position: absolute; | position: absolute; | ||
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left: 0px; | left: 0px; | ||
width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(T--Nanjing-China-- | + | background-image:url(https://static.igem.org/mediawiki/2016/6/66/T--Nanjing-China--home_project_4.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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− | #menu_project # | + | #menu_project #page3 #yeast-blue3 |
{ | { | ||
position: absolute; | position: absolute; | ||
− | top: | + | top: 160px; |
left: 255px; | left: 255px; | ||
width: 300px; | width: 300px; | ||
height: 300px; | height: 300px; | ||
− | background-image:url(T--Nanjing-China-- | + | background-image:url(https://static.igem.org/mediawiki/2016/1/1b/T--Nanjing-China--home_project_3.png); |
background-size:contain; | background-size:contain; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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</style> | </style> | ||
− | <div class="page" id=" | + | <div class="page" id="page3"> |
− | <div data-anchor-target="# | + | <div data-anchor-target="#page3" data--450-top="opacity: 1" data--650-top="opacity: 0"> |
− | <header data-anchor-target="# | + | <header data-anchor-target="#page3" data-250-top="opacity: 1" data-550-top="opacity: 0"> |
− | <h2> | + | <h2 style="padding-top:20px;padding-bottom:20px;">Encapsulation</h2> |
− | + | ||
</header> | </header> | ||
− | <div id="yeast- | + | <div id="yeast-red3" data-anchor-target="#page3 header h2" data-150-top="left: 100px; opacity: 1" data-550-top="left: 175px; opacity: 0"></div> |
− | <div id="yeast- | + | <div id="yeast-blue3" data-anchor-target="#page3 header h2" data-150-top="left: 355px; opacity: 1" data-550-top="left: 280px; opacity: 0"></div> |
− | <div data-anchor-target="# | + | <div data-anchor-target="#page3 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0"> |
<ul> | <ul> | ||
<li> | <li> | ||
− | < | + | <h6>5. Bacteria form microballs under silicon encapsulation and hydrogen evolves from anaerobic core under illumination.</h6> |
</li> | </li> | ||
</ul> | </ul> | ||
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</div> | </div> | ||
</div> | </div> | ||
− | </section | + | </section> |
<script type="text/javascript"> | <script type="text/javascript"> | ||
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<div class="bottom_main"> | <div class="bottom_main"> | ||
<div class="bottom_contact"> | <div class="bottom_contact"> | ||
− | <h4>CONTACT</h4> | + | <h4 style="padding-top:50px;padding-bottom:20px;">CONTACT</h4> |
<h5>Address</h5> | <h5>Address</h5> | ||
− | <h6> | + | <h6><ul><li>Life Science Department</li> |
− | Nanjing University< | + | <li>#163 Xianlin Blvd, Qixia District</li> |
− | Nanjing< | + | <li>Nanjing University</li> |
− | China | + | <li>Nanjing, Jiangsu Province</li> |
− | < | + | <li>P.R. of China</li> |
+ | <li>Zip: 210046</li></ul> | ||
</h6> | </h6> | ||
− | |||
− | |||
− | |||
<h5>Email</h5> | <h5>Email</h5> | ||
− | <h6> | + | <h6><ul><li>nanjing_china@163.com</li></ul> |
− | + | ||
</h6> | </h6> | ||
<h5>Social media</h5> | <h5>Social media</h5> | ||
− | <h6>Facebook<br /> | + | <h6><ul><li><a href="https://www.facebook.com/IGEM-Nanjing-China-392481144280345/">Facebook</a><br /></li> |
− | Twitter< | + | <li><a href="https://twitter.com/nanjing__china">Twitter</a></li></ul> |
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Latest revision as of 15:01, 19 October 2016
Abstract
Solar energy is the most abundant form of energy on earth, but is extremely hard to use. Thus scientists have been trying different methods to convert solar energy to chemical energy. To transfer light to allow sustainable production of chemicals motivates the development of artificial photosynthetic systems. Two main problems to be solved in this system are the electron transport media and enzymes to harvest excited electrons. The current transportation method either requires expensive semiconductor nanoparticles or special species which are inconvenient for gene operation.
What’s more, for downstream enzymes that harvest electrons, including hydrogenase and nitrogenase, we found that they are generally oxygen-intolerant. So we also want to build an oxygen-isolation system.
This year we managed to solve all the problems mentioned above once and forever in synthetic biology. Our project proposes a new method to construct an artificial photosynthetic system in model organism E.coli with the help of metal-binding proteins and surface display machinery. In addition, using silicon encapsulation, we overcome oxygen intolerance barriers of enzymes with assistance from polymer materials.
To test the efficiency of our artificial photosynthetic system and silicon encapsulation method, we use hydrogen production as an example. We successfully achieve aerobic hydrogen production using E.coli hydrogenase 1 under normal conditions. What is really exciting as that our new light-driven system can also be extended to more model species including yeast and bacillus, so do the encapsulation system!