Difference between revisions of "Team:Nanjing-China"

 
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     </div>
 
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     <div class="container_1_content">
 
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       <h6>Solar energy is the most abundant energy form on earth, but is hard to use.</h6>
+
       <h6>Solar energy is the most abundant form of energy on earth, but is extremely hard to use. Thus scientists have been trying different methods to convert solar energy to chemical energy. To transfer light to allow sustainable production of chemicals motivates the development of <span class="home_blue">artificial photosynthetic systems</span>. Two main problems to be solved in this system are the electron transport media and enzymes to harvest excited electrons. The current transportation method either requires expensive semiconductor nanoparticles or special species which are inconvenient for gene operation. </h6>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h6>To transfer light to allow sustainable production of chemicals motivates the development of artificial photosynthetic systems. The two key elements in this system are the electron transport media and enzymes to harvest excited electrons. The current transportation method either requires expensive semiconductor nanoparticles or special species inconvenient for gene operation. In which way can we make a new system with an easier as well as cheaper method? </h6>
+
<h6>What’s more, for downstream enzymes that harvest electrons, including hydrogenase and nitrogenase, we found that they are <span class="home_blue">generally oxygen-intolerant</span>. So we also want to build an oxygen-isolation system.</h6>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h6>What’s more, for downstream enzymes that harvest electrons, including hydrogenase and nitrogenase, we found that they are generally oxygen intolerant. So we also want to build an oxygen-isolation system.</h6>
+
<h6>This year we managed to solve all the problems mentioned above once and forever in synthetic biology. Our project proposes a new method to construct an <span class="home_blue">artificial photosynthetic system</span> in model organism <em>E.coli</em> with the help of metal-binding proteins and surface display machinery. In addition, using silicon encapsulation, we <span class="home_blue">overcome oxygen intolerance</span> barriers of enzymes with assistance from polymer materials. </h6>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h6>This year we managed to solve this problem once forever in synthetic biology.
+
<h6>To test the efficiency of our artificial photosynthetic system and silicon encapsulation method, we use hydrogen production as an example. We successfully achieve aerobic hydrogen production using <em>E.coli</em> hydrogenase 1 under normal conditions. What is really exciting as that our new light-driven system can also be extended to more model species including yeast and bacillus, so do the encapsulation system!</h6>
−
Our project proposes a new method to construct an artificial photosynthetic system in model organism <em>E.coli</em> with the help of metal-binding protein and surface display machinery. </h6>
+
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h6>In addition, using silicon encapsulation and charge effect, we have the ability to overcome oxygen intolerance with assist from polymer materials. With this clever strategy, we achieve in air hydrogen production using <em>E.coli</em> hydrogenase 1 in normal condition. </h6>
+
<h6>The flow chart below shows how our <strong>artificial photosynthetic system</strong> and <strong>silicon encapsulation system</strong> works corporately to achieve hydrogen production!</h6>
−
<p>&nbsp;</p>
+
−
<h6>This new light-driven system is of general applications which can also extend to more species including yeast and bacillus. So do the encapsulation system!</h6>
+
 
     </div>
 
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   </div>
 
   </div>
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       <h2>Title</h2>
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       <h2 style="padding-top:20px;padding-bottom:20px;">Subcloning</h2>
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      <p>Subtitle</p>
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     </header>
  
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           <h6>1. Plasmids encoding the OmpA-PbrR fused protein and Hya ABCDEF are co-transfered in to E.coli strain BL21(DE3).</h6>
 
         </li>
 
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       <h2 style="padding-top:20px;padding-bottom:20px;">PS System Construction</h2>
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     </header>
 
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           <h6>2. Induced expression of OmpA-PbrR and hydrogenase in order to construct our articifial photosynthetic system.</h6>
 
         </li>
 
         </li>
 
     </ul>
 
     </ul>
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           <h6>3. The light driven system is constructed by in situ formed CdS particles on the outer membrane of <em>E.coli.</em></h6>
 
         </li>
 
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       <h2 style="padding-top:20px;padding-bottom:20px;">LbL Assembly</h2>
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           <h6>4. Bacteria is warpped into multi-layered polymer materials.</h6>
 
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Latest revision as of 15:01, 19 October 2016

Home

Abstract

Solar energy is the most abundant form of energy on earth, but is extremely hard to use. Thus scientists have been trying different methods to convert solar energy to chemical energy. To transfer light to allow sustainable production of chemicals motivates the development of artificial photosynthetic systems. Two main problems to be solved in this system are the electron transport media and enzymes to harvest excited electrons. The current transportation method either requires expensive semiconductor nanoparticles or special species which are inconvenient for gene operation.

 

What’s more, for downstream enzymes that harvest electrons, including hydrogenase and nitrogenase, we found that they are generally oxygen-intolerant. So we also want to build an oxygen-isolation system.

 

This year we managed to solve all the problems mentioned above once and forever in synthetic biology. Our project proposes a new method to construct an artificial photosynthetic system in model organism E.coli with the help of metal-binding proteins and surface display machinery. In addition, using silicon encapsulation, we overcome oxygen intolerance barriers of enzymes with assistance from polymer materials.

 

To test the efficiency of our artificial photosynthetic system and silicon encapsulation method, we use hydrogen production as an example. We successfully achieve aerobic hydrogen production using E.coli hydrogenase 1 under normal conditions. What is really exciting as that our new light-driven system can also be extended to more model species including yeast and bacillus, so do the encapsulation system!

 

The flow chart below shows how our artificial photosynthetic system and silicon encapsulation system works corporately to achieve hydrogen production!

Quick Link

* Put your mouse on those cards to see what will happen

CONTACT

Address
  • Life Science Department
  • #163 Xianlin Blvd, Qixia District
  • Nanjing University
  • Nanjing, Jiangsu Province
  • P.R. of China
  • Zip: 210046
Email
  • nanjing_china@163.com
Social media

SPONSORS

南京大学
生命科学学院
SCHOOL OF LIFE SCIENCES
NANJING UNIVERSITY

南京大学

NANJING UNIVERSITY


南京大学
化学化工学院
SCHOOL OF CHEMISTRY AND CHEMICAL ENGINEERING
NANJING UNIVERSITY
南京大学
化学与生物医学科学研究所
INSTITUTE OF CHEMISTRY AND BIOMEDICAL SCIENCES
NANJING UNIVERSITY