Difference between revisions of "Team:Nanjing-China"

 
(35 intermediate revisions by the same user not shown)
Line 23: Line 23:
 
background-repeat: repeat;
 
background-repeat: repeat;
 
       }
 
       }
 +
.home_blue{
 +
color:#00a0ea;
 +
}
 
</style>
 
</style>
  
Line 125: Line 128:
 
     </div>
 
     </div>
 
     <div class="container_1_content">
 
     <div class="container_1_content">
−
       <h6>Solar energy is the most abundant energy form on earth, but is hard to use.</h6>
+
       <h6>Solar energy is the most abundant form of energy on earth, but is extremely hard to use. Thus scientists have been trying different methods to convert solar energy to chemical energy. To transfer light to allow sustainable production of chemicals motivates the development of <span class="home_blue">artificial photosynthetic systems</span>. Two main problems to be solved in this system are the electron transport media and enzymes to harvest excited electrons. The current transportation method either requires expensive semiconductor nanoparticles or special species which are inconvenient for gene operation. </h6>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h6>To transfer light to allow sustainable production of chemicals motivates the development of artificial photosynthetic systems. The two key elements in this system are the electron transport media and enzymes to harvest excited electrons. The current transportation method either requires expensive semiconductor nanoparticles or special species inconvenient for gene operation. In which way can we make a new system with an easier as well as cheaper method? </h6>
+
<h6>What’s more, for downstream enzymes that harvest electrons, including hydrogenase and nitrogenase, we found that they are <span class="home_blue">generally oxygen-intolerant</span>. So we also want to build an oxygen-isolation system.</h6>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h6>What’s more, for downstream enzymes that harvest electrons, including hydrogenase and nitrogenase, we found that they are generally oxygen intolerant. So we also want to build an oxygen-isolation system.</h6>
+
<h6>This year we managed to solve all the problems mentioned above once and forever in synthetic biology. Our project proposes a new method to construct an <span class="home_blue">artificial photosynthetic system</span> in model organism <em>E.coli</em> with the help of metal-binding proteins and surface display machinery. In addition, using silicon encapsulation, we <span class="home_blue">overcome oxygen intolerance</span> barriers of enzymes with assistance from polymer materials. </h6>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h6>This year we managed to solve this problem once forever in synthetic biology.
+
<h6>To test the efficiency of our artificial photosynthetic system and silicon encapsulation method, we use hydrogen production as an example. We successfully achieve aerobic hydrogen production using <em>E.coli</em> hydrogenase 1 under normal conditions. What is really exciting as that our new light-driven system can also be extended to more model species including yeast and bacillus, so do the encapsulation system!</h6>
−
Our project proposes a new method to construct an artificial photosynthetic system in model organism <em>E.coli</em> with the help of metal-binding protein and surface display machinery. </h6>
+
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h6>In addition, using silicon encapsulation and charge effect, we have the ability to overcome oxygen intolerance with assist from polymer materials. With this clever strategy, we achieve in air hydrogen production using <em>E.coli</em> hydrogenase 1 in normal condition. </h6>
+
<h6>The flow chart below shows how our <strong>artificial photosynthetic system</strong> and <strong>silicon encapsulation system</strong> works corporately to achieve hydrogen production!</h6>
−
<p>&nbsp;</p>
+
−
<h6>This new light-driven system is of general applications which can also extend to more species including yeast and bacillus. So do the encapsulation system!</h6>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 203: Line 203:
 
{
 
{
 
   padding:30px;
 
   padding:30px;
−
   height: 800px;
+
   height: 600px;
 
   position: relative;
 
   position: relative;
 
}
 
}
Line 240: Line 240:
 
#menu_project #page1 ul li:first-child  
 
#menu_project #page1 ul li:first-child  
 
{
 
{
−
   top: 300px;
+
   top: 200px;
 
   left: 600px;
 
   left: 600px;
 
   width: 600px;
 
   width: 600px;
Line 262: Line 262:
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 0px;
 
   left: 0px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(T--Nanjing-China--home_project_1.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/a/a9/T--Nanjing-China--home_project_1_2.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
Line 274: Line 274:
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 255px;
 
   left: 255px;
−
   width: 400px;
+
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(https://static.igem.org/mediawiki/2016/2/24/T--Nanjing-China--home_project_1.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/1/1f/T--Nanjing-China--home_project_1_1.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
Line 287: Line 287:
  
 
<div class="page" id="page1">
 
<div class="page" id="page1">
−
   <div data-anchor-target="#page1" data--400-top="opacity: 1" data--600-top="opacity: 0">
+
   <div data-anchor-target="#page1" data--450-top="opacity: 1" data--650-top="opacity: 0">
  
−
     <header data-anchor-target="#page1" data-200-top="opacity: 1" data-500-top="opacity: 0">
+
     <header data-anchor-target="#page1" data-250-top="opacity: 1" data-550-top="opacity: 0">
−
       <h2 style="padding-top:20px;padding-bottom:20px;">Title</h2>
+
       <h2 style="padding-top:20px;padding-bottom:20px;">Subcloning</h2>
 
     </header>
 
     </header>
  
−
<!--    <div id="yeast-red1" data-anchor-target="#page1 header h2" data-100-top="left: 0px; opacity: 1" data-500-top="left: 75px; opacity: 0"></div>
+
    <div id="yeast-red1" data-anchor-target="#page1 header h2" data-150-top="left: 250px; opacity: 1" data-550-top="left: 400px; opacity: 0"></div>
−
-->
+
     <div id="yeast-blue1" data-anchor-target="#page1 header h2" data-150-top="left: 263px; opacity: 1" data-550-top="left: 100px; opacity: 0"></div>
−
     <div id="yeast-blue1" data-anchor-target="#page1 header h2" data-100-top="left: 255px; opacity: 1" data-500-top="left: 180px; opacity: 0"></div>
+
  
−
     <div data-anchor-target="#page1 header h2" data-50-top="opacity: 1" data-150-top="opacity: 0">
+
     <div data-anchor-target="#page1 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0">
 
       <ul>
 
       <ul>
 
         <li>
 
         <li>
Line 312: Line 311:
 
<style>
 
<style>
  
−
#menu_project #page2
+
#menu_project #page4
 
{
 
{
 
   padding:30px;
 
   padding:30px;
−
   height: 800px;
+
   height: 600px;
 
   position: relative;
 
   position: relative;
 
}
 
}
  
−
#menu_project #page2 h2  
+
#menu_project #page4 h2  
 
{
 
{
 
   padding-top: 50px;
 
   padding-top: 50px;
Line 325: Line 324:
 
}
 
}
  
−
#menu_project #page2 header  
+
#menu_project #page4 header  
 
{
 
{
 
   padding: 0 55px;
 
   padding: 0 55px;
 
}
 
}
  
−
#menu_project #page2 header p  
+
#menu_project #page4 header p  
 
{
 
{
 
   font-size: 24px;
 
   font-size: 24px;
 
}
 
}
  
−
#menu_project #page2 ul  
+
#menu_project #page4 ul  
 
{
 
{
 
   margin: 0;
 
   margin: 0;
Line 342: Line 341:
 
}
 
}
  
−
#menu_project #page2 ul li  
+
#menu_project #page4 ul li  
 
{
 
{
 
   margin: 0;
 
   margin: 0;
Line 350: Line 349:
 
}
 
}
  
−
#menu_project #page2 ul li:first-child  
+
#menu_project #page4 ul li:first-child  
 
{
 
{
−
   top: 300px;
+
   top: 200px;
 
   left: 600px;
 
   left: 600px;
 
   width: 600px;
 
   width: 600px;
 
}
 
}
  
−
#menu_project #page2 #yeast-red2
+
#menu_project #page4 #yeast-red4
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 163px;
 
   left: 0px;
 
   left: 0px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(T--Nanjing-China--home_project_3.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/4/41/T--Nanjing-China--home_project_2_1.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
 
}
 
}
  
−
#menu_project #page2 #yeast-blue2
+
#menu_project #page4 #yeast-blue4
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 255px;
 
   left: 255px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(https://static.igem.org/mediawiki/2016/4/4b/T--Nanjing-China--home_project_2_2.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/8/8e/T--Nanjing-China--home_project_2_2_2.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
Line 384: Line 383:
 
</style>
 
</style>
  
−
<div class="page" id="page2">
+
<div class="page" id="page4">
−
   <div data-anchor-target="#page2" data--400-top="opacity: 1" data--600-top="opacity: 0">
+
   <div data-anchor-target="#page4" data--450-top="opacity: 1" data--650-top="opacity: 0">
  
−
     <header data-anchor-target="#page2" data-200-top="opacity: 1" data-500-top="opacity: 0">
+
     <header data-anchor-target="#page4" data-250-top="opacity: 1" data-550-top="opacity: 0">
−
       <h2 style="padding-top:20px;padding-bottom:20px;">Title</h2>
+
       <h2 style="padding-top:20px;padding-bottom:20px;">PS System Construction</h2>
 
     </header>
 
     </header>
  
−
<!--    <div id="yeast-red2" data-anchor-target="#page2 header h2" data-100-top="left: 0px; opacity: 1" data-500-top="left: 75px; opacity: 0"></div>
+
    <div id="yeast-red4" data-anchor-target="#page4 header h2" data-150-top="left: 231px; opacity: 1" data-550-top="left: 100px; opacity: 0"></div>
−
-->
+
 
−
     <div id="yeast-blue2" data-anchor-target="#page2 header h2" data-100-top="left: 255px; opacity: 1" data-500-top="left: 180px; opacity: 0"></div>
+
     <div id="yeast-blue4" data-anchor-target="#page4 header h2" data-150-top="left: 270px; opacity: 1" data-550-top="left: 400px; opacity: 0"></div>
  
−
     <div data-anchor-target="#page2 header h2" data-50-top="opacity: 1" data-150-top="opacity: 0">
+
     <div data-anchor-target="#page4 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0">
 
       <ul>
 
       <ul>
 
         <li>
 
         <li>
−
           <h6>2. Bacteria is warpped into multi-layered polymer materials.</h6>
+
           <h6>2. Induced expression of OmpA-PbrR and hydrogenase in order to construct our articifial photosynthetic system.</h6>
 
         </li>
 
         </li>
 
     </ul>
 
     </ul>
Line 410: Line 409:
 
<style>
 
<style>
  
−
#menu_project #page3
+
#menu_project #page2
 
{
 
{
 
   padding:30px;
 
   padding:30px;
−
   height: 800px;
+
   height: 600px;
 
   position: relative;
 
   position: relative;
 
}
 
}
  
−
#menu_project #page3 h2  
+
#menu_project #page2 h2  
 
{
 
{
 
   padding-top: 50px;
 
   padding-top: 50px;
Line 423: Line 422:
 
}
 
}
  
−
#menu_project #page3 header  
+
#menu_project #page2 header  
 
{
 
{
 
   padding: 0 55px;
 
   padding: 0 55px;
 
}
 
}
  
−
#menu_project #page3 header p  
+
#menu_project #page2 header p  
 
{
 
{
 
   font-size: 24px;
 
   font-size: 24px;
 
}
 
}
  
−
#menu_project #page3 ul  
+
#menu_project #page2 ul  
 
{
 
{
 
   margin: 0;
 
   margin: 0;
Line 440: Line 439:
 
}
 
}
  
−
#menu_project #page3 ul li  
+
#menu_project #page2 ul li  
 
{
 
{
 
   margin: 0;
 
   margin: 0;
Line 448: Line 447:
 
}
 
}
  
−
#menu_project #page3 ul li:first-child  
+
#menu_project #page2 ul li:first-child  
 
{
 
{
−
   top: 300px;
+
   top: 200px;
 
   left: 600px;
 
   left: 600px;
 
   width: 600px;
 
   width: 600px;
 
}
 
}
  
−
 
+
#menu_project #page2 #yeast-red2
−
#menu_project #page3 #yeast-red3
+
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 0px;
 
   left: 0px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(https://static.igem.org/mediawiki/2016/6/66/T--Nanjing-China--home_project_4.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/8/84/T--Nanjing-China--home_project_3_1.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
 
}
 
}
  
−
#menu_project #page3 #yeast-blue3
+
#menu_project #page2 #yeast-blue2
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 255px;
 
   left: 255px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(https://static.igem.org/mediawiki/2016/1/1b/T--Nanjing-China--home_project_3.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/5/54/T--Nanjing-China--home_project_3_2.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
Line 483: Line 481:
 
</style>
 
</style>
  
−
<div class="page" id="page3">
+
<div class="page" id="page2">
−
   <div data-anchor-target="#page3" data--400-top="opacity: 1" data--600-top="opacity: 0">
+
   <div data-anchor-target="#page2" data--450-top="opacity: 1" data--650-top="opacity: 0">
  
−
     <header data-anchor-target="#page3" data-200-top="opacity: 1" data-500-top="opacity: 0">
+
     <header data-anchor-target="#page2" data-250-top="opacity: 1" data-550-top="opacity: 0">
−
       <h2 style="padding-top:20px;padding-bottom:20px;">Title</h2>
+
       <h2 style="padding-top:20px;padding-bottom:20px;">In Situ Particles</h2>
 
     </header>
 
     </header>
  
−
     <div id="yeast-red3" data-anchor-target="#page3 header h2" data-100-top="left: 0px; opacity: 1" data-500-top="left: 75px; opacity: 0"></div>
+
     <div id="yeast-red2" data-anchor-target="#page2 header h2" data-150-top="left: 242px; opacity: 1" data-550-top="left: 100px; opacity: 0"></div>
 +
    <div id="yeast-blue2" data-anchor-target="#page2 header h2" data-150-top="left: 250px; opacity: 1" data-550-top="left: 400px; opacity: 0"></div>
  
−
     <div id="yeast-blue3" data-anchor-target="#page3 header h2" data-100-top="left: 255px; opacity: 1" data-500-top="left: 180px; opacity: 0"></div>
+
     <div data-anchor-target="#page2 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0">
−
 
+
−
    <div data-anchor-target="#page3 header h2" data-50-top="opacity: 1" data-150-top="opacity: 0">
+
 
       <ul>
 
       <ul>
 
         <li>
 
         <li>
−
           <h6>3. Bacteria form microballs under silicon encapsulation and hydrogen evolves from anaerobic core under illumination.</h6>
+
           <h6>3. The light driven system is constructed by in situ formed CdS particles on the outer membrane of <em>E.coli.</em></h6>
 
         </li>
 
         </li>
 
     </ul>
 
     </ul>
Line 509: Line 506:
 
<style>
 
<style>
  
−
#menu_project #page4
+
#menu_project #page5
 
{
 
{
 
   padding:30px;
 
   padding:30px;
−
   height: 800px;
+
   height: 600px;
 
   position: relative;
 
   position: relative;
 
}
 
}
  
−
#menu_project #page4 h2  
+
#menu_project #page5 h2  
 
{
 
{
 
   padding-top: 50px;
 
   padding-top: 50px;
Line 522: Line 519:
 
}
 
}
  
−
#menu_project #page4 header  
+
#menu_project #page5 header  
 
{
 
{
 
   padding: 0 55px;
 
   padding: 0 55px;
 
}
 
}
  
−
#menu_project #page4 header p  
+
#menu_project #page5 header p  
 
{
 
{
 
   font-size: 24px;
 
   font-size: 24px;
 
}
 
}
  
−
#menu_project #page4 ul  
+
#menu_project #page5 ul  
 
{
 
{
 
   margin: 0;
 
   margin: 0;
Line 539: Line 536:
 
}
 
}
  
−
#menu_project #page4 ul li  
+
#menu_project #page5 ul li  
 
{
 
{
 
   margin: 0;
 
   margin: 0;
Line 547: Line 544:
 
}
 
}
  
−
#menu_project #page4 ul li:first-child  
+
#menu_project #page5 ul li:first-child  
 
{
 
{
−
   top: 300px;
+
   top: 200px;
 
   left: 600px;
 
   left: 600px;
 
   width: 600px;
 
   width: 600px;
 
}
 
}
  
−
#menu_project #page4 #yeast-red4
+
#menu_project #page5 #yeast-red5
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 0px;
 
   left: 0px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(T--Nanjing-China--home_project_3.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/b/bf/T--Nanjing-China--home_project_4_1.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
 
}
 
}
  
−
#menu_project #page4 #yeast-blue4
+
#menu_project #page5 #yeast-blue5
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 255px;
 
   left: 255px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(T--Nanjing-China--home_project_4.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/5/5c/T--Nanjing-China--home_project_4_2.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
Line 581: Line 578:
 
</style>
 
</style>
  
−
<div class="page" id="page4">
+
<div class="page" id="page5">
−
   <div data-anchor-target="#page4" data--400-top="opacity: 1" data--600-top="opacity: 0">
+
   <div data-anchor-target="#page5" data--450-top="opacity: 1" data--650-top="opacity: 0">
  
−
     <header data-anchor-target="#page4" data-200-top="opacity: 1" data-500-top="opacity: 0">
+
     <header data-anchor-target="#page5" data-250-top="opacity: 1" data-550-top="opacity: 0">
−
       <h2 style="padding-top:20px;padding-bottom:20px;">Title</h2>
+
       <h2 style="padding-top:20px;padding-bottom:20px;">LbL Assembly</h2>
 
     </header>
 
     </header>
  
−
     <div id="yeast-red4" data-anchor-target="#page4 header h2" data-100-top="left: 0px; opacity: 1" data-500-top="left: 75px; opacity: 0"></div>
+
     <div id="yeast-red5" data-anchor-target="#page5 header h2" data-150-top="left: 237px; opacity: 1" data-550-top="left: 100px; opacity: 0"></div>
  
−
     <div id="yeast-blue4" data-anchor-target="#page4 header h2" data-100-top="left: 255px; opacity: 1" data-500-top="left: 180px; opacity: 0"></div>
+
     <div id="yeast-blue5" data-anchor-target="#page5 header h2" data-150-top="left: 265px; opacity: 1" data-550-top="left: 400px; opacity: 0"></div>
  
−
     <div data-anchor-target="#page4 header h2" data-50-top="opacity: 1" data-150-top="opacity: 0">
+
     <div data-anchor-target="#page5 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0">
 
       <ul>
 
       <ul>
 
         <li>
 
         <li>
−
           <h6>4. Induced expression of OmpA-PbrR and hydrogenase in order to construct our articifial photosynthetic system.</h6>
+
           <h6>4. Bacteria is warpped into multi-layered polymer materials.</h6>
 
         </li>
 
         </li>
 
     </ul>
 
     </ul>
Line 607: Line 604:
 
<style>
 
<style>
  
−
#menu_project #page5
+
#menu_project #page3
 
{
 
{
 
   padding:30px;
 
   padding:30px;
−
   height: 800px;
+
   height: 600px;
 
   position: relative;
 
   position: relative;
 
}
 
}
  
−
#menu_project #page5 h2  
+
#menu_project #page3 h2  
 
{
 
{
 
   padding-top: 50px;
 
   padding-top: 50px;
Line 620: Line 617:
 
}
 
}
  
−
#menu_project #page5 header  
+
#menu_project #page3 header  
 
{
 
{
 
   padding: 0 55px;
 
   padding: 0 55px;
 
}
 
}
  
−
#menu_project #page5 header p  
+
#menu_project #page3 header p  
 
{
 
{
 
   font-size: 24px;
 
   font-size: 24px;
 
}
 
}
  
−
#menu_project #page5 ul  
+
#menu_project #page3 ul  
 
{
 
{
 
   margin: 0;
 
   margin: 0;
Line 637: Line 634:
 
}
 
}
  
−
#menu_project #page5 ul li  
+
#menu_project #page3 ul li  
 
{
 
{
 
   margin: 0;
 
   margin: 0;
Line 645: Line 642:
 
}
 
}
  
−
#menu_project #page5 ul li:first-child  
+
#menu_project #page3 ul li:first-child  
 
{
 
{
−
   top: 300px;
+
   top: 200px;
 
   left: 600px;
 
   left: 600px;
 
   width: 600px;
 
   width: 600px;
 
}
 
}
  
−
#menu_project #page5 #yeast-red5
+
 
 +
#menu_project #page3 #yeast-red3
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 0px;
 
   left: 0px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(T--Nanjing-China--home_project_3.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/6/66/T--Nanjing-China--home_project_4.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
 
}
 
}
  
−
#menu_project #page5 #yeast-blue5
+
#menu_project #page3 #yeast-blue3
 
{
 
{
 
   position: absolute;
 
   position: absolute;
−
   top: 260px;
+
   top: 160px;
 
   left: 255px;
 
   left: 255px;
 
   width: 300px;
 
   width: 300px;
 
   height: 300px;
 
   height: 300px;
−
   background-image:url(T--Nanjing-China--home_project_4.png);
+
   background-image:url(https://static.igem.org/mediawiki/2016/1/1b/T--Nanjing-China--home_project_3.png);
 
   background-size:contain;
 
   background-size:contain;
 
   background-repeat: no-repeat;
 
   background-repeat: no-repeat;
Line 679: Line 677:
 
</style>
 
</style>
  
−
<div class="page" id="page5">
+
<div class="page" id="page3">
−
   <div data-anchor-target="#page5" data--400-top="opacity: 1" data--600-top="opacity: 0">
+
   <div data-anchor-target="#page3" data--450-top="opacity: 1" data--650-top="opacity: 0">
  
−
     <header data-anchor-target="#page5" data-200-top="opacity: 1" data-500-top="opacity: 0">
+
     <header data-anchor-target="#page3" data-250-top="opacity: 1" data-550-top="opacity: 0">
−
       <h2 style="padding-top:20px;padding-bottom:20px;">Title</h2>
+
       <h2 style="padding-top:20px;padding-bottom:20px;">Encapsulation</h2>
 
     </header>
 
     </header>
  
−
     <div id="yeast-red5" data-anchor-target="#page5 header h2" data-100-top="left: 0px; opacity: 1" data-500-top="left: 75px; opacity: 0"></div>
+
     <div id="yeast-red3" data-anchor-target="#page3 header h2" data-150-top="left: 100px; opacity: 1" data-550-top="left: 175px; opacity: 0"></div>
  
−
     <div id="yeast-blue5" data-anchor-target="#page5 header h2" data-100-top="left: 255px; opacity: 1" data-500-top="left: 180px; opacity: 0"></div>
+
     <div id="yeast-blue3" data-anchor-target="#page3 header h2" data-150-top="left: 355px; opacity: 1" data-550-top="left: 280px; opacity: 0"></div>
  
−
     <div data-anchor-target="#page5 header h2" data-50-top="opacity: 1" data-150-top="opacity: 0">
+
     <div data-anchor-target="#page3 header h2" data-150-top="opacity: 1" data-250-top="opacity: 0">
 
       <ul>
 
       <ul>
 
         <li>
 
         <li>
−
           <h6>5.The light driven system is constructed by in situ formed CdS particles on the outer membrane of E.coli.</h6>
+
           <h6>5. Bacteria form microballs under silicon encapsulation and hydrogen evolves from anaerobic core under illumination.</h6>
 
         </li>
 
         </li>
 
     </ul>
 
     </ul>
Line 815: Line 813:
 
     </h6>
 
     </h6>
 
     <h5>Email</h5>
 
     <h5>Email</h5>
−
     <h6><ul><li>nanjing_China@163.com</li></ul>
+
     <h6><ul><li>nanjing_china@163.com</li></ul>
 
     </h6>
 
     </h6>
 
     <h5>Social media</h5>
 
     <h5>Social media</h5>
−
     <h6><ul><li><a href="https://twitter.com/nanjing__china">Facebook</a><br /></li>
+
     <h6><ul><li><a href="https://www.facebook.com/IGEM-Nanjing-China-392481144280345/">Facebook</a><br /></li>
 
       <li><a href="https://twitter.com/nanjing__china">Twitter</a></li></ul>
 
       <li><a href="https://twitter.com/nanjing__china">Twitter</a></li></ul>
 
     </h6>
 
     </h6>

Latest revision as of 15:01, 19 October 2016

Home

Abstract

Solar energy is the most abundant form of energy on earth, but is extremely hard to use. Thus scientists have been trying different methods to convert solar energy to chemical energy. To transfer light to allow sustainable production of chemicals motivates the development of artificial photosynthetic systems. Two main problems to be solved in this system are the electron transport media and enzymes to harvest excited electrons. The current transportation method either requires expensive semiconductor nanoparticles or special species which are inconvenient for gene operation.

 

What’s more, for downstream enzymes that harvest electrons, including hydrogenase and nitrogenase, we found that they are generally oxygen-intolerant. So we also want to build an oxygen-isolation system.

 

This year we managed to solve all the problems mentioned above once and forever in synthetic biology. Our project proposes a new method to construct an artificial photosynthetic system in model organism E.coli with the help of metal-binding proteins and surface display machinery. In addition, using silicon encapsulation, we overcome oxygen intolerance barriers of enzymes with assistance from polymer materials.

 

To test the efficiency of our artificial photosynthetic system and silicon encapsulation method, we use hydrogen production as an example. We successfully achieve aerobic hydrogen production using E.coli hydrogenase 1 under normal conditions. What is really exciting as that our new light-driven system can also be extended to more model species including yeast and bacillus, so do the encapsulation system!

 

The flow chart below shows how our artificial photosynthetic system and silicon encapsulation system works corporately to achieve hydrogen production!

Quick Link

* Put your mouse on those cards to see what will happen

CONTACT

Address
  • Life Science Department
  • #163 Xianlin Blvd, Qixia District
  • Nanjing University
  • Nanjing, Jiangsu Province
  • P.R. of China
  • Zip: 210046
Email
  • nanjing_china@163.com
Social media

SPONSORS

南京大学
生命科学学院
SCHOOL OF LIFE SCIENCES
NANJING UNIVERSITY

南京大学

NANJING UNIVERSITY


南京大学
化学化工学院
SCHOOL OF CHEMISTRY AND CHEMICAL ENGINEERING
NANJING UNIVERSITY
南京大学
化学与生物医学科学研究所
INSTITUTE OF CHEMISTRY AND BIOMEDICAL SCIENCES
NANJING UNIVERSITY