Difference between revisions of "Team:Austin UTexas/Results"

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<p>For the next round of conjugation, we used a strain of both <i>G. oxydans</i> and  <i>Gluconacetobacter hansenii</i> from the American Type Culture Collection (ATCC).  Our procedure was identical to the one described in the previously paragraph.</p>
 
<p>For the next round of conjugation, we used a strain of both <i>G. oxydans</i> and  <i>Gluconacetobacter hansenii</i> from the American Type Culture Collection (ATCC).  Our procedure was identical to the one described in the previously paragraph.</p>
 
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[[File:T--Austin UTexas--LB+SPEC2ndconj.jpg|thumb|left|300px|'''Figure 1.''' These are our potential transconjugants on a LB+DAP plates. The dark reader was used when taking this picture. The top two are <i>G. oxydans</i> while the bottom two are <i>G. hansenii</i>.]]
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[[File:T--Austin UTexas--LB+SPEC2ndconj.jpg|thumb|center|300px|'''Figure 1.''' These are our potential transconjugants on a LB+DAP plates. The dark reader was used when taking this picture. The top two are <i>G. oxydans</i> while the bottom two are <i>G. hansenii</i>.]]
 
<html><p>We then picked isolated colonies and streaked them out onto LB+DAP plates. After using 16s sequencing on the potential transconjugants, we encountered an anomaly. Instead of amplifying the 16s gene, we received the sequence of the L,D Transpeptidase gene of <i> E. coli</i>.</p>
 
<html><p>We then picked isolated colonies and streaked them out onto LB+DAP plates. After using 16s sequencing on the potential transconjugants, we encountered an anomaly. Instead of amplifying the 16s gene, we received the sequence of the L,D Transpeptidase gene of <i> E. coli</i>.</p>
 
<p>We then decided to perform a Minimum Inhibitory Concentration (MIC) experiment in order to determine if <i>G. oxydans</i> is able to survive antibiotics above the standard <i>E. coli</i> concentration. We tested the antibiotics kanamycin, spectinomycin and carbenicillin.</p>
 
<p>We then decided to perform a Minimum Inhibitory Concentration (MIC) experiment in order to determine if <i>G. oxydans</i> is able to survive antibiotics above the standard <i>E. coli</i> concentration. We tested the antibiotics kanamycin, spectinomycin and carbenicillin.</p>

Revision as of 17:17, 19 October 2016

Results


Click on one of the images below to learn more about our results!