Difference between revisions of "Team:Manchester"

 
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<p style="font-size:8px;" class="font8">Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 10
 
<p style="font-size:8px;" class="font8">Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 10
 
undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University. We are based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.</p>
 
undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University. We are based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.</p>
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   <br />
 
   <br />
  
−
<p style="font-size:20px" class="font20">Self-awareness of alcohol consumption and intoxication levels can be difficult for anyone to master. Through the exploration of trying to find a niche within our society that our project could be beneficial to, our project’s targeted audience has changed dramatically since the initial idea of the AlcoPatch. We have found that our AlcoPatch would have many applications to the people within our society whose aim is to not actually get drunk! Our patch would provide these people with a visible colour change according to their blood alcohol concentration thus giving them the ability to monitor their personal intoxication levels. Having an indicator to show how intoxicated a person is could help reduce the undesired consequences of being too drunk, the knock-on effects of these to the public services, drink-driving, as well as the prevention of serving alcohol to people who are over the limit and much more. </p>   
+
<p style="font-size:20px" class="font20">Self-awareness of alcohol consumption and intoxication levels is surprisingly difficult to achieve. Our alcohol awareness tool, AlcoPatch, provides a <a href="https://2016.igem.org/Team:Manchester/Model/Costing" target="_blank"> cheap </a>, bio-based, sustainable and robust solution to help increasing self-awareness. The AlcoPatch would provide users with a visible colour signal indicating their blood alcohol concentration in real time. Throughout the project, we have worked closely with the <a href="https://2016.igem.org/Team:Manchester/Human_Practices" target="_blank">police, health care providers, charities</a> and potential users to design a product that would meet the real needs of communities around the world. Having a rapid and reliable indicator to show visually when one has had enough could help reduce the undesired consequences of being too drunk. This helps people who do not want to get drunk, but would also have positive knock-on effects to the people around them, from reducing public disturbance to avoiding drink-driving.
 +
</p>   
  
 
<p style="font-size:8px;" class="font8">Self-awareness of alcohol consumption and intoxication levels can be difficult for anyone to master. Through the exploration of trying to find a niche within our society that our project could be beneficial to, our project’s targeted audience has changed dramatically since the initial idea of the AlcoPatch. We have found that our AlcoPatch would have many applications to the people within our society whose aim is to not actually get drunk! Our patch would provide these people with a visible colour change according to their blood alcohol concentration thus giving them the ability to monitor their personal intoxication levels. Having an indicator to show how intoxicated a person is could help reduce the undesired consequences of being too drunk, the knock-on effects of these to the public services, drink-driving, as well as the prevention of serving alcohol to people who are over the limit and much more. </p>   
 
<p style="font-size:8px;" class="font8">Self-awareness of alcohol consumption and intoxication levels can be difficult for anyone to master. Through the exploration of trying to find a niche within our society that our project could be beneficial to, our project’s targeted audience has changed dramatically since the initial idea of the AlcoPatch. We have found that our AlcoPatch would have many applications to the people within our society whose aim is to not actually get drunk! Our patch would provide these people with a visible colour change according to their blood alcohol concentration thus giving them the ability to monitor their personal intoxication levels. Having an indicator to show how intoxicated a person is could help reduce the undesired consequences of being too drunk, the knock-on effects of these to the public services, drink-driving, as well as the prevention of serving alcohol to people who are over the limit and much more. </p>   
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<br /><br />
 
<br /><br />
 
<h4 style="color:gold">Science Behind AlcoPatch </h4>
 
<h4 style="color:gold">Science Behind AlcoPatch </h4>
−
<p style="font-size: 20px" class="font20">It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOx) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (<i>H<sub>2</sub>O<sub>2</sub></i>) as a by-product. <i>H<sub>2</sub>O<sub>2</sub></i> is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce luminescence. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein.
+
<p style="font-size: 20px" class="font20">It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration <sup>[3]</sup>. Based on this, we aim to develop an ethanol biosensor by using synthetic biology, based on two separate potential methods to measure intoxication levels. The <a href="https://2016.igem.org/Team:Manchester/Description/mechanism1" target="_blank"> first mechanism</a> involves the use of alcohol oxidase (AOx) in a <a href="https://2016.igem.org/Team:Manchester/Description/mechanism1" target="_blank"> cell-free system</a> to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as a by-product. H<sub>2</sub>O<sub>2</sub> is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce a gradient of the colour green. The <a href="https://2016.igem.org/Team:Manchester/Description/mechanism2" target="_blank"> second mechanism</a> involves activation of the ethanol-sensitive transcription factor <a href="http://parts.igem.org/Part:BBa_K2092004"target="_blank">alcR</a> in engineered <i>Escherichia coli (E. coli) </i>. The activated transcription factor then activates the <a href="http://parts.igem.org/Part:BBa_K2092003"target="_blank"> alcA promoter </a> leading to the expression of chromoproteins.
 +
 
 
</p>
 
</p>
  
−
<p style="font-size: 8px" class="font8">It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOx) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (<i>H<sub>2</sub>O<sub>2</sub></i>) as a by-product. <i>H<sub>2</sub>O<sub>2</sub></i> is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce luminescence. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein.
+
<p style="font-size: 8px" class="font8">It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOx) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as a by-product. H<sub>2</sub>O<sub>2</sub> is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce a colour change. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein.
 
</p>
 
</p>
 +
 +
<!--------------------------------------------------judging----------------------------------------------------------------->
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</br></br>
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 +
<h4 style="color:gold">Judging Criteria </h4>
 +
 +
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<div class="trophy1 column width33">
 +
  <center>
 +
  <img class="width80" src="https://static.igem.org/mediawiki/2016/1/13/T--Manchester--Trophy_Bronze.png" alt="Bronze Trophy" />
 +
  </center>
 +
 +
  <ul class="bronze_info">
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>See you at the Giant Jamboree!</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Created a <a href="https://2016.igem.org/Team:Manchester" target="_blank"> Team Wiki</a>. Welcome, you are currently on it! </li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Massive thank you to every individual who has <a href="https://2016.igem.org/Team:Manchester/Attributions" target="_blank"> contributed</a> towards our
 +
project. </li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Click to find out more about our <a href="https://2016.igem.org/Safety/Check_In?team_id=2092" target="_blank"> safety check-in</a>, <a href="https://2016.igem.org/Safety/About_Our_Lab?team_id=2092" target="_blank"> lab</a>, <a href="https://2016.igem.org/Safety/About_Our_Project?team_id=2092" target="_blank"> project</a>, <a href="https://2016.igem.org/Safety/Final_Safety_Form?team_id=2092" target="_blank"> final safety</a> and <a href="https://igem.org/2016_Judging_Form?id=2092" target="_blank"> judging form</a>.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Check out one of our new <a href="https://2016.igem.org/Team:Manchester/Parts" target="_blank"> parts</a>, <a href="http://parts.igem.org/Part:BBa_K2092000" target="_blank"> BBa_K2092000</a>, which has been submitted to the iGEM Registry.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Hope to see all of you at our poster and project presentation at the Giant Jamboree 2016!</li>
 +
 +
  </ul>
 +
 +
 +
</div>
 +
 +
<!--------------------------------------------------------------------------Sliver---------------------------------------------------------------------------------------------->
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<div class="trophy1 column width33">
 +
  <center>
 +
  <img class="width80" src="https://static.igem.org/mediawiki/2016/3/33/T--Manchester--Trophy_Sliver.png" alt="Bronze Trophy" />
 +
  </center>
 +
 +
  <ul class="bronze_info">
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--Tick_Sliver.png" alt="Tick" />
 +
    <li>Experimentally validated and showed success on our key BioBricks: <a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank"> BBa_K2092003</a>, <a href="http://parts.igem.org/Part:BBa_K2092004" target="_blank"> BBa_K2092004</a>, <a href="http://parts.igem.org/Part:BBa_K2092008" target="_blank"> BBa_K2092008</a> and <a href="http://parts.igem.org/Part:BBa_K2092009" target="_blank"> BBa_K2092009</a>.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--Tick_Sliver.png" alt="Tick" />
 +
    <li>Without the <a href="https://2016.igem.org/Team:Manchester/Collaborations" target="_blank"> collaboration</a> with other iGEM teams, our project would not have been successful. Thank you!</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--Tick_Sliver.png" alt="Tick" />
 +
    <li>Reached out to various <a href="https://2016.igem.org/Team:Manchester/Human_Practices" target="_blank"> stakeholders, charities, public services</a> and many others to make sure our device addresses real-world needs in a responsible and efficient way.</li>
 +
 +
  </ul> 
 +
 +
 +
</div>
 +
 +
<!--------------------------------------------------------------------------Gold---------------------------------------------------------------------------------------------->
 +
 +
<div class="trophy1 column width33">
 +
  <center>
 +
  <img class="width80" src="https://static.igem.org/mediawiki/2016/9/9b/T--Manchester--Trophy_Gold.png" alt="Bronze Trophy" />
 +
  </center>
 +
 +
    <ul class="bronze_info">
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/2/27/T--Manchester--Tick_Gold.png" alt="Tick" />
 +
    <li>Integrated the investigated issues into the design of your project by modelling the <a href="https://2016.igem.org/Team:Manchester/Model/Costing" target="_blank">cost analysis</a> of the AlcoPatch and finding suitable <a href="https://2016.igem.org/Team:Manchester/HP/Gold" target="_blank">alternatives </a> for risk-posing substances used in this project.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/2/27/T--Manchester--Tick_Gold.png" alt="Tick" />
 +
    <li><a href="https://2016.igem.org/Team:Manchester/Description/mechanism2#improved" target="_blank"> Improved</a> an existing BioBrick by adding two missing restriction sites – XbaI and SpeI making assembly process easier. </li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/2/27/T--Manchester--Tick_Gold.png" alt="Tick" />
 +
    <li>Characterised previously existing BioBricks <a href="http://parts.igem.org/Part:BBa_K1033930 " target="_blank">BBa_K1033930</a> and <a href="http://parts.igem.org/Part:BBa_K1033925" target="_blank">BBa_K1033925</a>.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/2/27/T--Manchester--Tick_Gold.png" alt="Tick" />
 +
    <li>Demonstrated a functional <a href="https://2016.igem.org/Team:Manchester/Proof" target="_blank"> proof of concept</a> of our project.  </li>
 +
  </ul>
 +
 +
</div>
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 +
<!----------------------------------------------------navigate---------------------------------------------------------->
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<div style="clear:both" class="column project">
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<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Description" title="Project Overview"><img src="https://static.igem.org/mediawiki/2016/b/b1/T--Manchester--mainpage_project.png" alt="Project Overview" /></a>
 +
<center>
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<h3><a href="https://2016.igem.org/Team:Manchester/Description">Project Overview</a></h3>
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</center>
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</div>
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<div class="column project">
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<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Achievement" title="Achievement Overview"><img src="https://static.igem.org/mediawiki/2016/3/37/T--Manchester--mainpage_achievement.png" alt="Achievement Overview" /></a>
 +
<center>
 +
<h3><a href="https://2016.igem.org/Team:Manchester/Achievement">Achievement Overview</a></h3>
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</center>
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</div>
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<div class="column project">
 +
<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Human_Practices" title="Human Practices Overview"><img src="https://static.igem.org/mediawiki/2016/d/d9/T--Manchester--mainpage_hp.png" alt="Human Practices Overview" /></a>
 +
<center>
 +
<h3><a href="https://2016.igem.org/Team:Manchester/Human_Practices">Human Practices Overview</a></h3>
 +
</center>
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</div>
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<div class="column project">
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<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Team" title="Team"><img src="https://static.igem.org/mediawiki/2016/8/83/T--Manchester--mainpage_team.png" alt="Team" /></a>
 +
<center>
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<h3><a class="projectlink" href="https://2016.igem.org/Team:Manchester/Team">Team</a></h3>
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</center>
 +
</div>
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<div class="column project">
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<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Model" title="Modelling Overview"><img src="https://static.igem.org/mediawiki/2016/5/5c/T--Manchester--mainpage_modelling.png" alt="Modelling Overview" /></a>
 +
<center>
 +
<h3><a href="https://2016.igem.org/Team:Manchester/Model">Modelling Overview</a></h3>
 +
</center>
 +
</div>
  
 
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−
<div class="social">
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</div>
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    <p class="com23" style="font-size:20px;">Our team attended the Boston iGEM Jamboree and we won a <a style="color:gold" href="https://2016.igem.org/Judging/Medals" target="_blank">Gold Medal</a> for our outstanding efforts, furthermore, we were presented with the prestigious <a href="https://2016.igem.org/Team:Manchester/Model" target="_blank">Best Model </a> award as well as being nominated for the <a href="https://2016.igem.org/Team:Manchester/Engagement" target="_blank">Best Education & Public Engagement</a>. </p>
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    <p class="mol23" style="font-size:9px;">Our team attended the Boston iGEM Jamboree and we won a <a style="color:gold" href="https://2016.igem.org/Judging/Medals" target="_blank">Gold Medal</a> for our outstanding efforts, furthermore, we were presented with the prestigious <a href="https://2016.igem.org/Team:Manchester/Model" target="_blank">Best Model </a> award as well as being nominated for the <a href="https://2016.igem.org/Team:Manchester/Engagement" target="_blank">Best Education & Public Engagement</a>. </p>
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  <li>Global status report on alcohol and health. 2014 edition. Geneva: World Health Organization; 2014. Available at: <a href="http://apps.who.int/iris/bitstream/10665/112736/1/9789240692763_eng.pdf" target="_blank"> http://apps.who.int/iris/bitstream/10665/112736/1/9789240692763_eng.pdf </a> [Accessed 14 October 2016]</li>
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<li>Alcohol concern, (2016). <i>Alcohol statistics</i>. [online]. Available at: <a href="http://www.alcoholconcern.org.uk/alcohol-statistics " target="_blank"> http://www.alcoholconcern.org.uk/alcohol-statistics</a> [Accessed 14 October 2016]</li>
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<li>Buono, M.J. (1999). ‘Sweat ethanol concentrations are highly correlated with co-existing blood values in humans’, <i>Experimental Physiology </i>, 84, 401-404.</li>
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Latest revision as of 19:46, 26 November 2016

IGEM Manchester Team 2016

Welcome to Manchester iGEM 2016!

group photo
group photo

Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 10 undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University. We are based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.

Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 10 undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University. We are based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.




Project Description



Self-awareness of alcohol consumption and intoxication levels is surprisingly difficult to achieve. Our alcohol awareness tool, AlcoPatch, provides a cheap , bio-based, sustainable and robust solution to help increasing self-awareness. The AlcoPatch would provide users with a visible colour signal indicating their blood alcohol concentration in real time. Throughout the project, we have worked closely with the police, health care providers, charities and potential users to design a product that would meet the real needs of communities around the world. Having a rapid and reliable indicator to show visually when one has had enough could help reduce the undesired consequences of being too drunk. This helps people who do not want to get drunk, but would also have positive knock-on effects to the people around them, from reducing public disturbance to avoiding drink-driving.

Self-awareness of alcohol consumption and intoxication levels can be difficult for anyone to master. Through the exploration of trying to find a niche within our society that our project could be beneficial to, our project’s targeted audience has changed dramatically since the initial idea of the AlcoPatch. We have found that our AlcoPatch would have many applications to the people within our society whose aim is to not actually get drunk! Our patch would provide these people with a visible colour change according to their blood alcohol concentration thus giving them the ability to monitor their personal intoxication levels. Having an indicator to show how intoxicated a person is could help reduce the undesired consequences of being too drunk, the knock-on effects of these to the public services, drink-driving, as well as the prevention of serving alcohol to people who are over the limit and much more.



Science Behind AlcoPatch

It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration [3]. Based on this, we aim to develop an ethanol biosensor by using synthetic biology, based on two separate potential methods to measure intoxication levels. The first mechanism involves the use of alcohol oxidase (AOx) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (H2O2) as a by-product. H2O2 is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce a gradient of the colour green. The second mechanism involves activation of the ethanol-sensitive transcription factor alcR in engineered Escherichia coli (E. coli) . The activated transcription factor then activates the alcA promoter leading to the expression of chromoproteins.

It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOx) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (H2O2) as a by-product. H2O2 is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce a colour change. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein.



Judging Criteria

Bronze Trophy
Bronze Trophy
Bronze Trophy
    Tick
  • Integrated the investigated issues into the design of your project by modelling the cost analysis of the AlcoPatch and finding suitable alternatives for risk-posing substances used in this project.
    • Tick
  • Improved an existing BioBrick by adding two missing restriction sites – XbaI and SpeI making assembly process easier.
    • Tick
  • Characterised previously existing BioBricks BBa_K1033930 and BBa_K1033925.
    • Tick
  • Demonstrated a functional proof of concept of our project.
Project Overview

Project Overview

Achievement Overview

Achievement Overview

Human Practices Overview

Human Practices Overview

Team

Team

Modelling Overview

Modelling Overview


2016 iGEM Giant Jamboree Results


Our team attended the Boston iGEM Jamboree and we won a Gold Medal for our outstanding efforts, furthermore, we were presented with the prestigious Best Model award as well as being nominated for the Best Education & Public Engagement.

Our team attended the Boston iGEM Jamboree and we won a Gold Medal for our outstanding efforts, furthermore, we were presented with the prestigious Best Model award as well as being nominated for the Best Education & Public Engagement.

Best Model Picture

References

Top ^

Sponsors

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