Team:Manchester/Parts
Parts
| BioBrick | Link | Part Name | Description | Part Type |
|---|---|---|---|---|
| BBa_K2092000 | http://parts.igem.org/Part:BBa_K2092000 | AOx (without RBS) | This part encodes for the AOx1 protein from Pichia pastoris. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NdeI restriction site between the prefix and the start of the AOx gene. | Basic |
| BBa_K2092001 | http://parts.igem.org/Part:BBa_K2092001 | alcR (without RBS/scar) | This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. | Basic |
| BBa_K2092002 | http://parts.igem.org/Part:BBa_K2092002 | PalcA | This part contains the ethanol/alcR regulated alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from iGEM DTU-Denmark 2011 | Basic |
| BBa_K2092003 | http://parts.igem.org/Part:BBa_K2092003 | PalcA(var) | This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression. The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native PalcA. | Basic |
| BBa_K2092004 | http://parts.igem.org/Part:BBa_K2092004 | alcR (incl. RBS) | This part is a twin of BBa_K2092001 with an additional RBS upstream of the start codon. This part encodes for AlcR protein, an ethanol-activated transcriptional factor from Aspergillus nidulans. It binds to alcA and aldA promoters to induce expression of proteins in the presence of ethanol or threonine. | Composite |
| BBa_K2092006 | http://parts.igem.org/Part:BBa_K2092006 | Pcon+RBS+amilCP | This part consists of a constitutive promoter, RBS and blue chromoprotein amilCP. Suitable to be used as a reporter. | Composite |
| BBa_K2092008 | http://parts.igem.org/Part:BBa_K2092008 | Pcon+RBS+alcR | This part consists of a constitutive promoter, RBS and alcR. Suitable to be used to measure the promoter strengths of alcA/aldA. | Composite |
| BBa_K2092009 | http://parts.igem.org/Part:BBa_K2092009 | PalcA(var)+RBS+amilCP | This part consists of PalcA(var) tagged with blue chromoprotein amilCP, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR. | Composite |
