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Revision as of 15:07, 16 August 2016
Notebook
Workspace
Our lab
We have our own iGEM TU Delft lab in the new Applied Science building on the edge of the TU Delft campus. It is classified as an ML-1 lab, the lowest safety level to work with modified organisms, which is enough for our experiments. Apart from this lab, we are also working in an optical lab, which is also ML-1 classified. In here built our own laser set-up.
Our office
Our office is our workplace for all the side tracks of our project, like the safety tool, the website, the model, all those nice things were created in here. Due to the moving to a new building, we had to wait a few months to use it. When we finally got it we made it our home and used the whole wall for nice discussions. Next to our office you can find our meeting room, where the meetings with our TA’s and PI’s took place.
Lab safety
Our lab is classified the lowest safety level (level 1), meaning that our experiments involve low or no risk. All the members of the team have successfully completed the following safety tests: Lab safety test General safety test of the building we work in Biological safety test for ML-1 lab General safety test of the old building we worked in All the members of the team have received safety training, including: Introduction to sterile working General lab training (using a PCR, making gels, etc.) General safety information, regarding contact persons and locations The safety of our experiments was supervised by Erwin van Rijn (Safety Manager of the lab) and Jeremie Capoulade (Safety Manager of the lasers). The supplies we needed in the lab were provided with the help of our instructor Esengül Yildirim. The research has been conducted with respect to the regulations of biosafety for The Netherlands, that can be found here.
Day Notes
DATE
participants
Notes
DATE
participants
Notes
DATE
participants
Notes
DATE
participants
Notes
10th August 2016
Tessa
Restricted PCR product of 3rd August, J23100 E0840, J23113 E0840 and J23117 E0840, with EcoRI-HF and PstI.
Purified restriction product.
Nanodropped purified product.
Nanodrop
| Product | Concentration (ng/ul) |
|---|---|
| J23100 E0840 | 14.7 |
| J23113 E0840 | 21.3 |
| J23117 E0840 | 23.3 |
Ligated purified product into pSB1C3.
Transformed ligation product into TOP10 strain. Using RFP as positive control and sterile MiliQ as negative control. Plated on plates with LB agar and CM.
11th August 2016
Tessa
Colony PCR'd colonies from yesterdays transformation.
Ran a 1% agarose gel of the PCR product.
Transferred colony 14 (J23113 E0840 pSB1C3) and 21 (J23117 E0840 pSB1C3) into liquid LB.
12th August 2016
Tessa
Miniprepped colony 14 and 21.
Nanodropped miniprep product.
Nanodrop
| Product | Concentration (ng/ul) |
|---|---|
| J23113 E0840 in pSB1C3 | 140.6 |
| J23117 E0840 in pSB1C3 | 325.6 |
Cryostocked colony 14 and 21.
Send miniprepped product for sequencing. [Sequence Confirmed]
