Difference between revisions of "Team:Austin UTexas/Parts"
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<h4>CpxA-CpxR</h4> | <h4>CpxA-CpxR</h4> | ||
| − | <p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. The | + | <p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. <b>The BioBrick can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097000">K2097000</a></b>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results#section6">here</a>.</p> |
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<h4>Blue Chromoprotein</h4> | <h4>Blue Chromoprotein</h4> | ||
| − | <p>This is a composite part made up of <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> (blue chromoprotein) and <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> is made up of a promoter, <a href="http://partsregistry.org/Part:BBa_J23104"> BBa_J23104</a>, and an RBS, <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_B0034</a>. The | + | <p>This is a composite part made up of <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> (blue chromoprotein) and <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> is made up of a promoter, <a href="http://partsregistry.org/Part:BBa_J23104"> BBa_J23104</a>, and an RBS, <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_B0034</a>. <b>The BioBrick can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097001">K2097001</a></b>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a>.</p> |
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<h4>Yellow-Green Chromoprotein</h4> | <h4>Yellow-Green Chromoprotein</h4> | ||
| − | <p>This is a composite part made up of <a href="http://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> (amajLime, a yellow-green chromoprotein) and <a href="http://parts.igem.org/Part:BBa_K608006"> BBa_K608006</a> (a medium promoter and RBS). It is made up of a medium promoter, <a href="http://parts.igem.org/Part:BBa_J23110"> BBa_J23110</a>, and a medium RBS, <a href="http://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>. The | + | <p>This is a composite part made up of <a href="http://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> (amajLime, a yellow-green chromoprotein) and <a href="http://parts.igem.org/Part:BBa_K608006"> BBa_K608006</a> (a medium promoter and RBS). It is made up of a medium promoter, <a href="http://parts.igem.org/Part:BBa_J23110"> BBa_J23110</a>, and a medium RBS, <a href="http://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>. <b>The BioBrick can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097002">K2097002</a></b>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a></p> |
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Revision as of 15:25, 19 October 2016
Austin_UTexas
Registry Parts
pH Sensors
CpxA-CpxR
CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in Shigella sonnei, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. The BioBrick can be found on the iGEM registry as: K2097000, as well as on our results page here.
Composite Parts
Blue Chromoprotein
This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B0034. The BioBrick can be found on the iGEM registry as: K2097001, as well as on our results page here.
Yellow-Green Chromoprotein
This is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein) and BBa_K608006 (a medium promoter and RBS). It is made up of a medium promoter, BBa_J23110, and a medium RBS, BBa_B0032. The BioBrick can be found on the iGEM registry as: K2097002, as well as on our results page here
