Team:William and Mary

Circuit Control

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creating a toolbox allowing for precise circuit control

The Toolbox

Our project provides a modular collection of genetic parts which can specifically and predictably tune the behavior of an arbitrary genetic circuit. This collection, which we have dubbed the “Circuit Control Toolbox,” consists of a suite of parts which can be added to the end of a given genetic circuit; each part provides a specific and independently tunable response which allows direct control over the ultimate output behavior of the circuit.

You can view our project description page here.

RBS

RBS Tuning

Synthetic Enhancer

Synthetic Enhancer Suite

RiboJ

Ribozyme Insulator

Binding Array

Decoy Binding Array

Parts

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This year, we submitted 118 parts to the BioBrick Registry.

These are the highlights.

Basic Part

The 85x tetO array

Composite Part

Two-step synthetic enhancer + NRII mutant

Part Collection

The circuit control toolbox

All Parts

All the parts we've made this year

Results

This summer we were able to build and test all components of our Circuit Control Toolbox. Find out more about our work here.

More paragraphs can be made here, if necessary.

Collaboration

Over the summer we were able to collaborate with great teams across the country. We spearheaded an effort to better character the RBS Community Collection with help from Alverno_CA and Pittsburgh, and we worked with Broad Run on their modeling efforts.

References