Team:IIT-Madras/Parts
Composite Parts
We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. These parts include Promoter-RBS composite parts, dual fluorescence reporter devices, RIBOS devices and sgRNA parts. To know more about the characterization and functionality of these parts and their future applications.
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Part Collection
We have submitted one part collection for efficient characterization of devices e.g. noise & strength. This part collection contains 12 composite parts. To know more about the characterization and functionality of these parts and their future applications.
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Size Characterization
Following is the gel picture of our constructs, P represents plasmid DNA and D represents plasmid DNA digested with EcoRI-HF and PstI:
Table of important parts
| Part Name | Part Number |
|---|---|
| pL-RBS 1 | [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001] |
| pL-RBS 2 | [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002] |
| pL-RBS 3 | [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003] |
| pL-RBS 4 | [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004] |
| pL-RIBOSOFF | [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005] |
| pL-gRNA1_CamR | [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006] |
| pL-gRNA2_CamR | [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007] |
| pL-gRNA1_mCherry | [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008] |
| pL-gRNA2_mCherry | [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009] |
| pL-gRNA_KanR | [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010] |
| pL-gRNA1_CamR-DT | [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011] |
| pL-gRNA1_mCherry-DT | [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012] |
| pL-gRNA_KanR-DT | [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013] |
| Noise in Device Kit 3 | [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014] |
| Noise in Device kit 4 | [http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015] |
| Expression vector for efficient Characterization | [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016] |
| Noise in Device kit 5 | [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017] |
| Noise in Device kit 6 | [http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018] |
| RIBOSON for mCherry RNA with K1956016 | [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] |
| RIBOSOFF 1 for lacI RNA with K1956016 | [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020] |
| RIBOSOFF2_lacIlacI-K1956016 | [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] |
| Noise in Device Kit 1 | [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022] |
| Noise in Device Kit 2 | [http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023] |
| LacI expression device | [http://parts.igem.org/Part:BBa_K1956034 BBa_K1956034] |
