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<title>PCR diary for pDEST mCherry with GG cassettes 2.0 · Benchling</title> ...data-cid="notetitleeditor" data-index="0">PCR diary for pDEST mCherry with GG cassettes 2.0</div></h1><div><div><i class="faded">Made with Benchling</i><

<left><h3><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000(GG 95):</a></h3></left> <left><h3><a href="http://parts.igem.org/Part:BBa_K2145001">BBa_K2145001(GG 96):</a></h3></left>

...tiline="false" data-cid="notetitleeditor" data-index="0">TN 06/28/16 PCR + GG</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>P ...ft" data-cid="table-s11332-1-0" data-namespace="table-ns16665"><span>pDONR-GG L1_L2</span></td><td class="cell is-text is-alignLeft" data-cid="table-s113

...left:0;">I diluted the gBlocks down to 5fmol/ul and used it to prepare the GG mixture </div></div></div><div class="mediocre-item is-text"><div class="me ...ft" data-cid="table-s12545-1-0" data-namespace="table-ns16847"><span>pDONR-GG L1_L2</span></td><td class="cell is-text is-alignLeft" data-cid="table-s125

...Left" data-cid="table-s140-1-0" data-namespace="table-ns15976"><span>pDONR-GG L1_L2</span></td><td class="cell is-text is-alignLeft" data-cid="table-s140 ...70">Miniprep Concentrations</span></b> - 1 is SloxP-SV40 GG, 2 is FRT-SV40 GG</div></div></div><div class="mediocre-item is-text"><div class="mediocre-no

..."ggggg","GGGG","GGGGG"],function(a,b,c,d){b[d.substr(0,2)]=q(a)}),R(["gg","GG"],function(b,c,d,e){c[e]=a.parseTwoDigitYear(b)}),H("Q",0,0,"quarter"),z("q

<li>TorA m1-1 GG </li> <li>TorA m1-2 GG </li>

...false" data-cid="note-part4183" data-index="243" style="margin-left:35px;">GG Sap1 digest</div></div></div><div class="mediocre-item is-text"><div class= ...false" data-cid="note-part4211" data-index="270" style="margin-left:35px;">GG Sap1 plates are super robust - should have plated with a lower dilution.</d

<h5>• DNA reaction (either parts=PUCTV, or PCR check for GG plasmids)</h5> <h5>(Example with Golden Gate Assembly Protocol for GG37-52; multiple GG Assembly for Plasmids can be done at a time in different tubes as seen in e

<h5>- PCR of GG Assemblies</h5> <h5>- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts</h5>

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.#{$fa-css-prefix}-gg:before { content: $fa-var-gg; } .#{$fa-css-prefix}-gg-circle:before { content: $fa-var-gg-circle; }

.@{fa-css-prefix}-gg:before { content: @fa-var-gg; } .@{fa-css-prefix}-gg-circle:before { content: @fa-var-gg-circle; }

.#{$fa-css-prefix}-gg:before { content: $fa-var-gg; } .#{$fa-css-prefix}-gg-circle:before { content: $fa-var-gg-circle; }

.fa-gg:before { .fa-gg-circle:before {

...com","https://maps.googleapis.com",null,"https://maps.google.com","https://gg.google.com","https://maps.gstatic.com/maps-api-v3/api/images/","https://www ...lf,of,qf,rf,uf,wf,Nf,Of,Pf,Qf,Rf,Sf,Uf,Vf,Wf,Xf,Zf,dg,fg,og,pg,vg,tg,wg,xg,Gg,Jg,Kg,Og,Pg,Sg,Tg,Ug,Vg,Wg,Da,Ea;_.aa="ERROR";_.ba="INVALID_REQUEST";_.ca="

...ft" data-cid="table-s13942-1-0" data-namespace="table-ns17066"><span>pDONR-GG L1_L2</span></td><td class="cell is-text is-alignLeft" data-cid="table-s139

<li>3 ul GG control w/ no ligase on Amp</li> <li>3 uL GG control w/ no insert on Amp</li>

..."><p class="c2 c1"><span class="c12 c87">C</span><span class="c15 c31 c12">GG</span></p><p class="c2 c1 c6"><span class="c15 c31 c12"></span></p><p class

.gg a:link img { .gg a:visited img {