Team:Alverno CA/Notebook

Alverno iGEM 2016

Notebook

Week of June 6th (Limited amount of notes taken)

- Lots of Designing Parts and Primers

- Wiki Work

Week of June 13th:

- Cleaning Restriction Digest and Ligation Done

- Ampicillin plates made

- Ampicillin stock made

- Transform RFP plasmid

- Design Parts and Primers

- Wiki Work

Week of June 20th

- Transformation Done (probably GFP)

- RFP and GRP colonies

- PCR for RFP Coding Gene

- Design Parts and Primers

- Wiki Work

Week of June 27th

- PCR Done for promoters, terminators, some UC parts

- Primer Dilutions Done

- Gel Run with PCR product from Week of June 20th (RFP)

- Miniprep kit for RFP Bba

- TBE Buffer 10x and 1x made

- PCR Purification done on Promoters and Terminators

- Wiki Work

Week of July 4th

- PCR of Vectors, some UC parts

- Primer Dilutions

- Gel Run UC16-18 and UC2 (UC18 ran twice)

- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)

- Plate Reader

- Wiki Work

Week of July 11th

- Learn how to use Benchling

- Primers Diluted

- Parts Diluted

- PCR done on P1ab, P2ab, T3ab, T4ab

- Gel Run on P1ab, P2ab, T3ab, T4ab

- PCR Clean up done on P1ab, P2ab, T3ab, T4ab

- Chloramphenicol plates made

- Wiki Work

Week of July 18th

- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)

- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d

- Transformation Done

- Chloramphenicol plates made

- Parts dilutions Done

- PCR of GG Assemblies

- PCR of UC3a-UC9a

- Wiki Work

Week of July 25th

- Set up Plate reader

- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts

- PCR Purification of UC3-9, V1a & V2a

- Picked Colonies

- Transformation Protocol for GG1-8

- Gel made & Gel made w/ SYBR safe

- PCR of GG Assemblies

- PCR for V1a and V2a, UC7-9

- Dilutions Done

- Gel Run for UC7-9

- Golden Gate Assembly GG9-16

- Wiki Work

Week of August 1st

- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23

- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12

- PCR Purification for UC7-8

- Parts Dilutions done UC2 & UC3-6 & UC7-8

- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23

- Fixed Thermocycler

- Gels Made

- Agar Plates made w/ Kan

- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26

- Transformation with GG12

- Ludox Calibration

- Test Fluorescence of Plasmids

- Wiki Work

Week of August 8th

- Cell Measurement

- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR

- PCR Check for GG24-26 & GG27-28

- Made Gel

- Transformation with GG24-26 & GG27-28

- Golden Gate Assembly GG27-28

- Colony PCR Check of GG24-26

- Primer Dilutions

- Plasmid DNA Purification

- LB Kan Broth

- Wiki Work

Week of August 15th

- Lab Meet-up with UCL/LC/CV

- Colony PCR Check for GG libraries 27-28

- Gels Made

- Gel Run for GG Library 28

- Grow Cultures GG27

- Learn Python

- Check Fluorescence for GG28 & GG27

- PCR UC14a

- Purification of Plasmid DNA

- Wiki Work

Week of August 22nd

- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36

- Gels Made

- PCR UC13a and UC15a (Done twice) & UC1

- PCR Purification of UC14 & UC13/15 & UC1

- Golden Gate Assembly for GG29-36

- PCR Check for GG29-36

- LB Kan Broth Made

- Dilution of UC1

- Control run in Plate Reader

- Wiki Work

Week of August 29th

- Make Gels

- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a

- Golden Gate Assembly GG37-52 & GG53-60

- PCR Check GG37-52 & GG53-60

- PCR Check Gel run for GG37-52

- PCR CDS1a & CDS2a

- PCR Purification of CDS1a & CDS2a

- Talk with iGEM Peshawar

- LB Kan Plates Made

- Wiki Work

Week of September 5th

- iGEM Team Meeting

- PCR Purification of CDS1a & CDS2a and UC1

- Transformation was done with GG53-60

- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64

- Gels made

- Dilution of CDS1a & CDS2a

- Golden Gate Assembly GG61-64

- PCR of UC1

- LB Kan Plates Made

- PCR Check for GG61-64

- TBE 10x Buffer

- Wiki Work

Week of September 12th

- Talked with UCL iGEM Team

- LV-LC-CV meet-up

- Google Hangouts with Lambert Georgia iGEM team

- Dilution of UC1a

- Transformation of GG29-36

- Golden Gate Assembly GG65-72

- PCR GG61-64

- PCR Check GG61-64

- TX-TL of GFP (+ control)

- Outreach workshop with Middle school

- Wiki Work

Week of September 19th

- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE

- Run Gel for GG61-64 (done twice) and GG65-72

- LB Kan Plates Made

- Gels Made

- TX-TL GFP Re-run

- Gel Run for UC1 and GG61-64

- Control Test ran using only the ladder for Gels

- TX-TL W & M plasmids

- PCR U3a

- Dilution of Primers

- Wiki Work

Week of September 26th

- PCR Purification of V3a

- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94

- PCR Check GG73-74 and GG75-82

- Dilution V3a

- Run Gel V3a

- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74

- TX-TL re-run for W & M plasmids

- Colony PCR GG61-64 and GG65-72

- LB Chloramphenicol plates & LB Kanamycin plates

- Wiki Work

Week of October 3rd

- Middle School Visit

- LB Broth made

- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab

- Dilution V3a

- PCR Check GG83-90

- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab

- TX-TL re-run for W & M plasmids w/ Replicates

- Gels Made

- Mini prep GG28 1-5

- Make TBE 1x