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..."text-align: justify;">We started to construct parts BBa_K1995003: HG (GFP-HG), BBa_K1995006: 2C (GFP-C) and BBa_K1995009: 2H (GFP-H).</p> ...digestion and finally got the ideal result.We linked BBa_K1995003: HG (GFP-HG), BBa_K1995006: 2C (GFP-C) and BBa_K1995009: 2H (GFP-H) to the vector.

...e fluorescence of GFP-MC, implying that the interaction between GFP-MC and Hg²⁺ is reversible (Figure 3-C).Similarly, there is a similar resul ...p>2+</sup>, at varying concentrations. (B) Fluorescence responses of IC to Hg²⁺ at varying concentrations. (C) Fluorescence titration of IC (O

...A, mercuric ion reductase, responsible for reducing mercury from Hg(II) to Hg(0), its volatile and less toxical form.</p> ...zes the break of binding between organical radicals and mercury, releasing Hg(II).</p>

...i>. Bacterial resistance to mercury occurs due to proteins that can act in Hg capture. Among those metal-chelating proteins, there is phytochelatin. </p> ...40FF00">Metal Binding Peptide</a>. In this project, we design a system for Hg bioaccumulation by a synthetic phytochelatin anchored in the membrane prote

...es rice HG-380MF-BBPC (b);HPLC analysis diagram of samples extract from HG-WT rice (c);<br><br> ...G-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div>

...B5/HZ,sCAIF,4C9B0+HL,4CCmBC,MAAO,KACP,iBAAkB,Q6B1/HZ,wCAxCR,8C9BohIC,8C8Bt+HG,MAAA,K9By+HH,iBAAA,YCmBD,+B6Bz/HM,aAAA,KAGA,qCApDN,qC9B8hIC,iBAAA,KCmBD,yC6

...B5/HZ,sCAIF,4C9B0+HL,4CCmBC,MAAO,KACP,iBAAkB,Q6B1/HZ,wCAxCR,8C9BohIC,8C8Bt+HG,MAAA,K9By+HH,iBAAA,YCmBD,+B6Bz/HM,aAAA,KAGA,qCApDN,qC9B8hIC,iBAAA,KCmBD,yC6

...ties are intimately connected in MerR role as regulator. C-terminal is the Hg binding site, mediated by three cysteine residues. Then, N-terminal is link ...tarts. The formation of pre-transcriptional complex allows quick answer to Hg(II) presence in the medium. The regulatory mechanism of merR and activation

...add Cu²⁺ in H-2 to the final concentration 25mg/L (Fig 1E), add Hg²⁺ to the other group to the final concentration 12.5mg/L (Fig 1C ...sup>2+</sup> (D)CC + Hg²⁺ (E)IH + Cu²⁺(F)IC + IPTG + Hg²⁺

...add Cu²⁺ in H-2 to the final concentration 25mg/L (Fig 1E), add Hg²⁺ to the other group to the final concentration 12.5mg/L (Fig 1C ...sup>2+</sup> (D)CC + Hg²⁺ (E)IH + Cu²⁺(F)IC + IPTG + Hg²⁺

...h 0.85% NaCl. Finally, cells were re-suspended in 0.85% NaCl. Solutions of Hg²⁺ and Cu²⁺ (1–10 μL) of different concentrations w ...To measure the selectivity of the E. coli to adsorb mercury ions, 6.5 mg/l Hg²⁺ and Cd²⁺, and 12.5 mg/l Cu²⁺ and Zn<sup>

<td>INP-N (N-terminal of Ice Nucleation Protein) + GFP + HG (His-Pro-Gly-His-Trp-Gly)</td> <td>INP-N (N-terminal of Ice Nucleation Protein) + HG (His-Pro-Gly-His-Trp-Gly)</td>

<tr><td>hG/A549</td><td>ADH-hAR-F/R</td><td>58</td></tr> <tr><td>2</td><td>GAL-hAR-Rhis/GAL-hAR-F</td><td>TRP-ADH-hAR</td><td>hG DNA</td></tr>

...collection include 8 promoters, all of them regulated by merR, sensible to Hg. They are: 1) Ptac1- for the log phase; 2) Ptac2 - a variation from Ptac1, ...degradation in 18 hours of bacterial growth. In the Bioreactor we had 50% Hg degradation in the first 18 hours.</p>

...1:a:1.$note-part5989.1.0.1.0.0.3.0.1.1.$2.$1"><span>-also reacts to Cd and Hg ...2.1">-they made a MerR/ZntR hybrid protein that recognizes Zn with reduced Hg response (see figure 4)</div></div></div><div class="mediocre-item is-text"

...HP;A+B1+HK;;;EAGE,YAAA;EACA,8BAAA;C/B4+HP;A+BphID;EA8CI,mBAAA;C/By+HH;A+Bx+HG;;EAEE,uBAAA;C/B0+HL;A+B3hID;EAoDM,uBAAA;C/B0+HL;A+B9hID;;EA0DI,sBAAA;C/Bw+H

...HP;A+B1+HK;;;EAGE,YAAA;EACA,8BAAA;C/B4+HP;A+BphID;EA8CI,mBAAA;C/By+HH;A+Bx+HG;;EAEE,uBAAA;C/B0+HL;A+B3hID;EAoDM,uBAAA;C/B0+HL;A+B9hID;;EA0DI,sBAAA;C/Bw+H

...a,_(cW,_(cX,cY,cZ,[_(cX,da,db,g,dc,[_(dd,de,cX,hF,dg,_(dh,di,dj,_(dk,dl,dm,hG,dp,[]),dq,g),dr,ds)])])),dN,bm,bB,g),_(bd,hl,bf,hH,bh,bi,eM,es,eN,eO,w,bj,b ...新窗口/新标签 打开 https://2016.igem.org/Team:SCUT-China_A/Safety",hG="https://2016.igem.org/Team:SCUT-China_A/Safety",hH="Team Members",hI="d120

<td width="124" height="48" align="center" valign="middle">HG-WT </td> <td width="145" align="center" valign="middle">HG-380MF-BBPC</td>

...fB,hu),hv,_(fB,hw),hx,_(fB,hy),hz,_(fB,hA),hB,_(fB,hC),hD,_(fB,hE),hF,_(fB,hG),hH,_(fB,hI),hJ,_(fB,hK),hL,_(fB,hM),hN,_(fB,hO),hP,_(fB,hQ),hR,_(fB,hS),hT ...0a87a34efeae73f71a5478b8be",hE="u53",hF="cce2c9e5b394488988841c291a61d3b8",hG="u54",hH="fd3606d5bced48a2880b1c82073a2ce3",hI="u55",hJ="b0f5cba392fd45559f