Team:Bielefeld-CeBiTec/Parts
Our Parts
Favorite Parts!
Best Basic Part
BBa_K2082106: Error-prone Polymerase I
For higher the variety of our Evobody library, mutation is a necessary step. One big problem in case of mutation systems is the arbitrarily mutation rate of the mutation proteins on the whole DNA. It is not able to control where mutation can happen. This error-prone polymerase I, a mutated E. coli DNA-Polymerase I, shows very rare mutations in the genomic DNA and spared the mutation of important proteins for the cell. With this part we want to create a possibilty for increased usage of an in vivo mutagenesis system implemented in the iGEM parts registry and usable by all coming iGEM teams. For further informations visit our Best Basic Part site.
Best Composite Part
BBa_K2082231:
Together with the part BBa_K2082221 the part BBa_K2082231 is one very important factor of our created bacterial two-hybrid system. It contains not only one fusion protein for the system, it also contains the whole modified reporter construct with the binding site for the cI protein of phage 434. With this part we want to make it possible for every coming iGEM member to create their own bacterial two-hybrid system for in vivo selection directly in E. coli. To get better informations about what we have achieved with this part you can go on our Best Composite Part site.
Best Part Collection
BBa_K2082000, BBa_K2082004, BBa_K2082005: Monobody construction kit
For the iGEM community we designed not 10 or 100 coherently parts. Our part collection includes 100,000 distinct sequences of special binding proteins, namely monobodies. We, the team iGEM Bielefeld 2016, want to enrich the iGEM community with a whole library of monobodies freely usable for everyone. With this collection we do not only integrate a new category in the iGEM part registry database, we implemented a whole fundamental framework composed on the monobody constant regions and RFP instead of the variable regions, which can easily exchanged for creating a specific monobody or even an own library. To round off the library we do not only create a framework for monobodies, we also created a similar framework for nanobodies, too (BBa_K208001,BBa_K208006). For more informations about our part collection visit our Best Part Collection site.
Improve a part
DnaQ is part of the DNA polymerase III and responsible for the proofreading activity of this complex. The dnaQ926 variant
loses this activity through mutation of two function essential amino acids inside the active site. The complete loss of proofreading as well as the resulting saturation of mismatch-repair makes dnaQ926 the single strongest mutator gene known (Fijalkowska und Schaaper 1996)
dnaQ926 is part of our genome wide mutator BBa_K2082117, but we also used it as a standalone mutator in BBa_K2082116. After initially designing our genome wide mutator we looked for mutagensis gene already in the iGEM partsreg and found several candidates. The most prominent one is iGEM SYSU-China 2014's dnaQ926 BBa_K1333108. Furthermore, we use iGEM Uchicago IGSB2014's emrR and dam in our large genome wide mutator.
For the full characterization you can visit the Improve a part page or the registry entry of the origin part.
dnaQ926 is part of our genome wide mutator BBa_K2082117, but we also used it as a standalone mutator in BBa_K2082116. After initially designing our genome wide mutator we looked for mutagensis gene already in the iGEM partsreg and found several candidates. The most prominent one is iGEM SYSU-China 2014's dnaQ926 BBa_K1333108. Furthermore, we use iGEM Uchicago IGSB2014's emrR and dam in our large genome wide mutator.
For the full characterization you can visit the Improve a part page or the registry entry of the origin part.
Library Parts
Monobody: Constant regions and RFP (exchange with variable regions to create your library)
Nanobody: Constant regions (insert variable regions to create your defined Nanobody or library)
Split CFP Bimolecular fluorescence complementation (BiFC) with anti-GFP-Nanobody
Split CFP Bimolecular fluorescence complementation (BiFC) with GFP
Monobody: Fusion protein of Monobody constant regions and RFP (exchange with variable regions to cre
Monobody library: Fusion proteins of Monobody and omega subunit of RNA polymerase (rpoZ)
Nanobody library: Fusion proteins of Nanobody and omega subunit of RNA polymerase (rpoZ)
Zika E-Protein
Zika E-Protein: Fusion of Zika E-Protein with cI (434) regulatory protein with cMyc linker
Monobody: Example binding protein
Nanobody: Example binding protein
Mutation Parts
Uracil glycosylase inhibitor
Cytidin deaminase 1 from Pteromyzon marinus
Error-prone polymerase I
Wild-type polymerase I
Weak RBS - dnaQ926 cds - terminator
Assembly of six mutator genes, each with weak promoter; terminal terminator
Modified Pbad
Stop-GFP (K3*) under control of constitutive promoter
Stop-GFP (Y106*) under control of constitutive promoter
Stop-GFP (K107*) under control of constitutive promoter
dnaQ926 under control of a modified Pbad
Six mutator genes under control of a modified Pbad
AraC-Pbad(mod)-RBS-EPPolI-Ter
AraC-Pbad(mod)-RBS-WTPolI-Ter
E1010-Barcode
Stop-GFP (K3*)
Stop-GFP (Y106*)
Stop-GFP (K107*)
early stop beta-lactamase
early stop beta-lactamase
Promotor, RBS, late stop beta-lactamase and terminator
later stop beta-lactamase
Selection Parts
Fusion protein of monobody HA4 and omega subunit of RNA polymerase (rpoZ)
Fusion protein of mutated monobody HA4(Y87A) and omega subunit of RNA polymerase (rpoZ)
Fusion protein of mutated monobody HA4(R38A) and omega subunit of RNA polymerase (rpoZ)
Fusion protein of double mutated monobody HA4(R38A,E52A) and omega subunit of RNA polymerase (rpoZ)
Fusion protein of SH2 domain of Abl1 and cI repressor protein of phage 434
Fusion protein of SH2 domain of Abl1 and cI repressor protein of phage 434 with triple alanine linker
Fusion protein of SH2 domain of Abl1 and cI repressor protein of phage 434 with cMyc linker
Fusion protein of regulatory protein Gal4 and the omega subunit of the RNA polymerase
Fusion protein of modificated regulatory protein Gal11P and cI of phage 434
Optimized lacZ promotor with binding site of regulatory protein cI of phage 434
RFP under the control of an optimized lacZ promotor
Fusion protein of HA4 and RpoZ under the control of an Anderson promotor
Fusion protein of mutated HA4(Y87A) and RpoZ under the control of an Anderson promotor
Fusion protein of mutated HA4(R38A) and RpoZ under the control of an Anderson promotor
Fusion protein of double mutated HA4(R38A,E52A) and RpoZ under the control of an Anderson promotor
Fusion protein of SH2 and cI (434) repressor protein under the control of an anderson promotor
Fusion protein of Gal4 and RpoZ under the control of an anderson promotor
Fusion protein of Gal11P and cI (434) repressor protein under control of an anderson promotor
RFP under the control of an optimized lacZ promotor combined with an fusion protein cassette of SH2 and cI(434)
RFP under the control of an optimized lacZ promotor combined with an fusion protein cassette of Gal11P and cI
RFP and Tetracycline resistence under the control of a modified lacZ promotor and fusion protein SH2
Beta-lactamase under the control of an modified lacZ promoter
BBa:K2082231 expanded by a second cI binding site OR2
Sequence for rpoZ Knock-Out in E. coli through CRISPR/Cas9
RFP under the control of an optimized lacZ promotor and SH2-cI(lambda) fusion protein
Tetracycline resistance
Red fluorescence protein
Beta-lactamase
Modified lacZ promoter with OR1 and OR2 binding sites for cI(434)
Modified lacZ promoter with OR1 binding site for cI of lambda
SH2 domain fused with cI of phage lambda
Our Parts in the Registry
