| Name |
Type |
Description |
Length |
| K1946000 |
Basic |
ChnR: This part codes for ChnR protein which is a Xyls/AraC-type transcriptional activator from Acinetobacter sp. It responds to the presence of cyclohexanone by inducing expression of ChnB protein. |
942 |
| K1946001 |
Basic |
pChnB: This part is the region starting from 537bp upstream of ChnB to its start codon from Acinetobacter sp. cyclohenol gene cluster. pChnB is activated by ChnR (K1946000) in the presence of cyclohexanone. |
537 |
| K1946002 |
Basic |
sgRNA targeting LacI: This part has a sgRNA targeting lacI. It also has 5' and 3' ribozyme which cleave the RNA molecule with self catalysis so that any sequence can be added to 5' or 3' to this part without preventing Cas9 binding and its activity. |
205 |
| K1946003 |
Basic |
Cycloxehanone Sensor: This part consists codon optimised ChnR (BBa_K1946000) from acinetobacter sp. operated by Tet operator and promoter region of ChnB (BBa_K1946001) followed by sfGFP (BBa_I746916). It is shown that ChnR activates ChnB protein in the presence of cyclohexanone. This sensor generates sfGFP in the presence of cyclohexanone. |
2535 |
| K1946004 |
Basic |
Toluene/Xylene Sensor: This part responds to the presence of toluene, m-, p- or o-xylenes and produces a green fluorescent signal. The transcriptional regulatory protein XylR recognizes these organic molecules and activates pu promoter which in turn will lead to the synthesis of sfGFP. |
3087 |
| K1946005 |
Basic |
XylR: This part codes for the transcriptional regulatory protein XylR from Pseudomonas putida. It activates the TOL plasmid xyl operons in the presence of toluene, m-, p- and o-xylene. |
1701 |
| K1946006 |
Basic |
pu: pu promoter drives transcription of the upper operon of Pseudomonas putida mt-2 TOL plasmid for degradation of toluene and xylenes. pu is activated by the activator protein XylR (BBa_K1946005) in the presence of toluene and xylenes. |
322 |
