Team:Dundee/Composite Part

Dundee 2016

Composite Parts

Composite Parts

Find out more about our devices

BBa_K1962010 (Bile Salts Sensing Device)

This is a composite part which consists of the bile salt responsive promoter (BBa_K318514) and a combined ribosome binding site / GFP / terminators biobrick (BBa_E0840). The acrRA operon is found in Salmonella enterica strain LT2 and the promoter sequence contains a RamA (BBa_K1962009) binding sequence.

BBa_K1962011 (A device for expression of Im-Ia in response to bile salts)

This is a composite part which consists of a bile salt responsive promoter (BBa_K318514), a RBS (BBa_B0030) and the biobrick encoding the Immunity Protein to Colicin Ia (BBa_K1962001). For best results use in conjunction with the RamA transcriptional activator (BBa_K1962009). The promoter activity has been chracterised using a GFP reporter (BBa_K1962010).

BBa_K1962012 (A device for expression of Im-E3 in response to bile salts)

This is a composite part that is designed to induce production of the Immunity Protein to Colicin E3 in the presence of bile. The composite part consists of the bile salt responsive promoter (BBa_K318514), RBS (BBa_B0030) and the Colicin E3 Immunity protein (BBa_K1962004). For best results, use in conjunction with the transcriptional activator RamA (BBa_K1962009).

BBa_K1962013 (A pH Sensing Device Based on The gadA Promoter)

This is a composite part that allows further characterisation of the pH sensitive gadA promoter from E. coli. The composite part comprises the gadA promoter(BBa_K1231001) and a combined RBS / GFP/ terminators biobrick (BBa_E0840).

BBa_K1962014 (A pH Sensing Device Based on The asr Promoter)

The "acid shock RNA" asr promoter is a pH sensitive promoter native to E. coli that has been documented by other iGEM teams. Here, we present a composite part to allow further characterisation of promoter activity. The composite part consists of the asr promoter(BBa_K1231000) and a combined biobrick containing RBS / GFP / terminators (BBa_E0840).

BBa_K1962015 (A device for expression of Im-Ia in response to pH)

This is a composite part for the production of the Immunity Protein to Colicin Ia in response to environmental pH changes. The composite part consists of a pH sensitive promoter (asr from biobrick BBa_K1231000, an RBS (BBa_B0030) and the Immunity Protein required to neutralise the bacteriocin activity of Colicin Ia (BBa_K1962001).

BBa_K1962016 (A device for expression of Im-E3 in response to pH)

This is a composite part for the regulated expression of the Immunity Protein for netralization of the Colicin E3, which is a specific RNase. The composite part was assembled from a pH responsive promoter (BBa_K1231000), an RBS (BBa_B0030) and the biobrick encoding the Immunity Protein against E3 (Im-E3) (BBa_K1962004).