Team:Hong Kong HKU/Parts
Achievements
We spent the entire Summer in the lab, and such hard work resulted in some achievements. We have submitted parts to the registry, experimentally validated our new BioBrick devices and demonstrated the assembly of our design and fulfilled the criteria for a gold medal.
Parts
Featured Parts
BBa_K2054000 - ssDNAs of our nanostructure
Forwarding our all submitted parts, we agree that it would be better if there is mighty single biobrick that encodes the all components of our desired structure.
And here we have it – putting all together .
BBa_K2054006 - ssDNAs of our simplified nanostructure
Oligos 1 and 5 are in a single plasmid to encode for the simplified version of our nanostructure.
Table of Parts
| Type | Name (Part number) | Type | Description | Length(bp) |
|---|---|---|---|---|
| Composite Part (Featured) |
BBa_K2054000 | DNA | ALL oligos included in our desired nanostructure | 3561 |
| Basic Part | BBa_K2054001 | DNA | Oligo 1 for the Tetrahedral nanostructure, w/ split G-quadruplex | 2388 |
| Basic Part | BBa_K2054002 | DNA | Oligo 2 for the Tetrahedral nanostructure | 2358 |
| Basic Part | BBa_K2054003 | DNA | Oligo 3 of the Tetrahedral nanostructure | 2375 |
| Basic Part | BBa_K2054004 | DNA | Oligo 4 of the Tetrahedral nanostructure | 2375 |
| Basic Part | BBa_K2054005 | DNA | Oligo 5 of the Tetrahedral nanostructure | 2313 |
| Composite Part (Featured) |
BBa_K2054006 | DNA | Oligos of the simplified 2D nanostructure | 2639 |
Achievements
For Bronze Medal
✓
Team Registration and Giant Jamboree Attendance (where we have prepared a 20-minutes presentation and a poster)
✓
Wiki page to document our project
✓
Project Attribution (Wiki page/About/Attributions)
✓
Safety Forms submission (Safety Check-in not required as all materials and parts that we are working with are on the White List)
✓
Judging Form submission
✓
New Part submitted to the Registry and documented on the Wiki page (Wiki page/Achievements/Parts):BBa_K 2054000-BBa_K20540006 [Received, Accepted]
For Silver Medal
✓
Submit a new part to the iGEM Parts Registry and document its characterization in the Main Page section of that corresponding Part Registry entry: BBa_KBBa_K2054001
✓
Experimentally validated the detection function of our tetrahedron nanostructure assembled from Parts:
BBa_K2054001, BBa_K2054002, BBa_K2054003, BBa_K2054004 and BBa_K2054005,
constructs produced signals in ABTS assays as expected
✓
Mentoring the new high school iGEM Team (Team Hong_Kong_UCCKE)
✓
Public Engagement Human Practices: Public exhibition at JSSE, and sharing iGEM experience with local high school students (at UCCKE campus)
For Gold Medal
✓
Inspired from an integrated Human Practice
The concept DNA nanostructure assembly and miRNA detection were integrated into our public exhibition at The 49th Joint School Science Exhibition.
Feedbacks from visitors offered us great inspiration the future application of our constructs and the potential social impacts of our project.
The creation of the Facebook video "Biosafety and Research Ethics- Conversation with Profession Danny Chan" inspired us to conduct a thorough risk assessment on E. coli DH10beta, which is a crucial component of our project.
✓
Demostrate the proof of concept
We used our nanostructure to test on the RNA inputs and mutants and our nanostructure worked as expected.
✓
Demonstrate
Our nanostructure passed tests on RNA inputs to fulfill real life condition.
