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2016 HKUST Troika

                                         

Home	About	Project	Modelling	Further Studies	Human Practices	Achievements	Lab	Collaborations	Interlab
	Team Attributions	Description phlFp tetp lacp Characterisation Results Prototype Result Parts Improvement	Model Stability Analysis Prediction Model	Potential Improvement	Human Practices Product Design Education Difficulties faced by iGEM teams Intellectual Property Rights Insights from Tristable Switch	Parts Medal Requirements	Protocols and Logbook Safety	Collaboration NUS IIT Madras Rice University

pTet Module

Hello guys welcome to the pTet Module!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?

Promoter, Repressor and Inducer Specification

• Promoter name: pTet (Tet repressible promoter)
• Length: 54 base pairs
• Strength with:
  • Weak RBS (BBa_B0032): 5.6 - 517.5 (+17) RPU
  • Medium RBS (BBa_B0030): 25.5 - 2323.1 (+259.5) RPU
  • Strong RBS (BBa_B0034): 39.6 - 4041.5 (+247.8) RPU

• Repressor name: TetR (Tet repressor)
• Part Length: 685 base pairs
• Structure: Homodimer of alpha-helices
• Binding affinity: To pTet, very light, 0-1 µl/ml has been shown to cause a 5 order of magnitude change in fluorescent protein production

• Inducer name: ATc (Anhydrotetracycline)
• Binding Affinity: (to TetR) Ka ~ 109 M-1
• Effective range: 250 - 25000 ng/ml

Mechanism of pTet - TetR - ATc Interaction