Team:JSNU-China/notebookpage

modeling

Notebook

July 10th~12th

  1. Be familiar with experimental steps(including the configuration of soft AGAR,western blot) and listed experiment required list
  2. Revived cells,inoculation and expansion
  3. Select an appropriate auxin

July 13th

  1. Configuration reagents used in this experiment
  2. Autoclaving reagent and experiment apparatus
  3. Observing cell metamorphosis

July 14th

  1. Configurate the lower gum of SDS-PAGE
  2. Observing cell metamorphosis

July 15th

  1. Configurate the supernatant gum of SDS-PAGE,extract protein, electrophoresis, trarsmembrane, primary antibody, secondary antibody, visualization and observe
  2. the configuration of soft AGAR
  3. Observing cell metamorphosis

July 16th

  1. Re extracting protein and do western blot
  2. Observing cell metamorphosis
  3. finish the configuration of soft AGAR
  4. Preparation of solid medium, in preparation for the transfection

July 17th

  1. Finished the rest of western blot, got the result of the experience
  2. Collected T293 cells and saving them by freezing

July 18th

  1. Tried to make T protein band by western blot, but it failed

July 19th

  1. Collected protein extraction cancer cells we need urgently
  2. Did western blot twice to test the actin of gastric cancer cell and Hybrid system cancer cells separately
  3. Collected the information of Competent cells

July 20th

  1. Did two western blot experiments, which were 193, 231,hela and hela, 231, to the end of primary antibody incubation
  2. Transformed competent cells, into two boards, PX300F and PX300

July 21th

  1. The transformation of PX300F failed, transformed it again
  2. The transformation of PX300F succeeded, shaked bacteria for the night, prepared extract plasmid
  3. Finished yesterday’s rest of western blot, second incubation resistance enhancement, analyzed the result of the experiment
  4. Observed the cells, collected T293 cells and saved them by freezing

July 22th

  1. Did two western blot. The first experiment, did two pieces of gel to test T protein and KLF4 protein in gastric cancer cell lines. The other one, did two pieces of gel to test T protein and actin in hybrid system cancer cells
  2. Because concentration was too low, the extraction of PX300 plasmid failed. Cultivated cells by shaking cells, prepared to extract plasmids. Coated PX300F plates succeeded, selected cloning cells to cultivate by shaking cells

July 23th

  1. The result of yesterday’s western blot was unsatisfactory, tested partly T protein and actin in hybrid system cancer cells again
  2. Extracted PX300 and PX300F plasmids

July 24th

  1. Finished the rest of yesterday’s western blot, analyzed the result of the experiment