Team:KAIT Japan/Labnote

Labonote

8/17

Restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
Transcription

8/18

Transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051

8/23

restriction emzyme fragmation

8/24

3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051

8/25

Miniprep
J04450

8/26

Restriction enzyme treatment

8/29

Purity a gel

8/30

PCR

8/31

PCR
3A assembly
K595024 + I14044

9/1

PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015

9/2

Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/3

Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/5

Restriction enzyme treatment

9/6

PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/7

Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/8

Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/8

PCR

9/12

Gel purification

9/13

Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment

9/14

Restriction enzyme treatment
Miniprep
K592024 I14044 J04450

9/15

Transformation
Restriction enzyme treatment
K592024 I14044 J04450

9/16

PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation

9/19

Miniprep

9/21

Miniprep

9/22

Restriction emzyme fragmation
Miniprep
Purify a gel

9/23

Miniprep
Purify a gel

9/24

Restriction emzyme fragmation

9/25

Purify a gel
Ligation
Transformation

9/26

Miniprep

9/27

Purify a gel
Restriction emzyme fragmation

9/28

Restriction emzyme fragmation

9/30

Restriction emzyme fragmation
Purify a gel

10/1

Ligation
transformation
Pre-culture
Purification of antisensence

10/2

Pre-culture
Miniprep
Electrophoresis to confirm

10/3

miniprep
PCR
Pre-culture
Purify a gel

10/4

Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm

10/5

Ligation
Transformation
Purify a gel
Restriction emzyme fragmation

10/6

KIB…miniprep
Electrophoresis to confirm
Purify a gel

10/7

Restriction emzyme fragmation
Ligation
Transformation

10/8

Miniprep
Purify a gel
Restriction emzyme fragmation

10/9

Pre-culture
Purify a gel

10/10

Ligation
Transformation

10/11

pre-culture
Miniprep

10/12

Purify a gel

10/13

Electrophoresis to confirm

10/14

Restriction emzyme fragmation

10/15

Electrophoresis to confirm
Restriction emzyme fragmation

10/16

Electrophoresis to confirm