Manchester iGEM 2016

Glucose Oxidase Reaction

Oxidation of glucose to Glucono-δ-lactone and Hydrogen Peroxide using Glucose Oxidase (EC 1.1.3.4)

Chemical Equation

$${Glucose} + {Oxygen} \rightleftharpoons {Glucano-} \delta {-lactone} + {Hydrogen} \ {Peroxide}$$

Rate Equation

$$\nu = \frac{K_{cat}[GOx] \frac{[Glucose]}{K_{m,Glucose}}\left({ 1- \frac{[Glucose]}{[H_2 O_2][{GDL}]} \frac{1}{K_{eq}}} \right)}{1+\frac{[Glucose]}{K_{m,Glucose}}+\frac{[H_2 O_2]}{K_{m,H_2 O_2}}+\frac{[H_2 O_2][GDL]}{K_{m,GDL}K_{m,H_2 O_2}}+\frac{[GLD]}{K_{m,GDL}}} $$

where:

Symbol	Meaning
$$K_{m,A}$$	Michaelis constant of species A
$$K_{cat}$$	Turnover number
$$[A]$$	Concentration of A
$$ \nu$$	Reaction Rate

Parameter Sourcing

K_cat and K_m parameters were collected from BRENDA

K_eq was calculated using the Van’t Hoff equation using the Gibbs free energy sourced from MetaCyc

$$ K_{eq} = e^{\frac{-\Delta G} {RT}}$$

Parameters with uncertainty

K_m

Probability density function for the Km of Glucose Oxidase

Uncertainty in K_m is introduced through the range of experimental parameters sourced. Properties of the raw data can be seen in the table below:

Property	Value
Minimum	0.815 mM
Maximum	733 mM
Mean	43.3 mM
Parameters Sourced	88

K_cat

Uncertainty in K_cat is introduced through the range of experimental parameters sourced. Properties of the raw data can be seen in the table below:

Property	Value
Minimum	0.03 s^-1
Maximum	2003 s^-1
Mean	617.3 s^-1
Parameters Sourced	33

K_eq

Probability density function for the Keq of Glucose Oxidase

Uncertainty in K_eq is introduced using a uniform range of temperature variations (298 K ± 5 K). Properties of this can be seen in the table below:

Property	Value
$$- \Delta G$$	140.833456736 kJ mol^-1
Minimum Temperature	293 K
Maximum Temperature	303 K

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Team:Manchester/Model/GlucoseOxidaseReaction

Glucose Oxidase Reaction

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