Team:NYMU-Taipei/Notebook-Lab Book-pBAR pMCL1 KR TtrpC

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9/11

  • pBAR_KR transformed into competent cell

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    9/12

  • pBAR_KR performed plasmid extraction and plasmid PCR (successful)

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    9/14

  • K118400 and TtrpC used PCR to amplify the target sequence

  • KR_TtrpC used PCR to amplify the target sequence

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    9/15

  • KR_TtrpC digested with restriction enzymes

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    9/16

  • pBAR_KR_TtrpC transformed into competent cell

  • Transformed pBAR_KR_TtrpC into competent cell

  • Digested pBAR_KR for transforming into Metarhizium anisopliae

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    9/17

  • pBAR_KR_TtrpC did 3 in 1

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    9/18

  • pBAR_KR_TtrpC did plasmid PCR and digestion check

  • Extracted plasmid pBAR_KR_TtrpC

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    9/20

  • KR_TtrpC digestion

  • Transformed pBAR_KR_TtrpC into competent cell

  • pBAR_KR_TtrpC did 3 in 1

  • Extracted plasmid pBAR_KR_TtrpC

  • pBAR_KR_TtrpC did digestion check

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    9/21

  • pBAR_KR_TtrpC did digestion check

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    9/22

  • Extracted plasmid pBAR_KR_TtrpC

  • pBAR_KR_TtrpC did plasmid PCR

  • pMCL1 and pBAR_KR_TtrpC digestion (pMCL1 was not digested successfully)

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    9/25

  • pMCL1 and pBAR_KR_TtrpC digestion

  • Transformed pBAR_pMCL1_KR_TtrpC into competent cell

  • pBAR_pMCL1_KR_TtrpC did 3 in 1

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    9/26

  • Extracted plasmid pBAR_pMCL1_KR_TtrpC

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    9/28

  • pBAR_pMCL1_KR_TtrpC did digestion check

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    9/30

  • pBAR_ Pmcl1_KR_TtrpC did digestion check

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    10/4

  • pBAR_KR_TtrpC digestion

  • Transformed pBAR_ Pmcl1_KR_TtrpC into competent cells

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    10/5

  • pBAR_ Pmcl1_KR_TtrpC containing cultures used in 2 in 1

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    10/6

  • Extracted plasmid pBAR_ Pmcl1_KR_TtrpC

  • pBAR_ Pmcl1_KR_TtrpC digestion check

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    10/8

  • pBAR_ Pmcl1_KR_TtrpC checked with restriction enzyme digestion (failure)

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