Next year, we are going to improve our project. We have 2 points.
I:We study about Lambda Red.
II:We study about CRISPR/Cas9.
[http://aem.asm.org/content/81/15/5103.short [9]Pyne ME, Moo-Young M, Chung DA, Chou CP. 2015. Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli. Applied and Environmental Microbiology 81:5103–5114.