Saturday, September 10th
Tasks:
Jordan & Tyler
- Ran a Gibson on Cas9-Lrz-SS and TorA (with PCR-added homology)
- Made 5 mL overnight cultures of:
- 10 colonies from Cas9-DsbA Gibson assembly product with PCR-added homology to insert
- 2 colonies from Cas9-DsbA Gibson assembly product with original DsbA gBlock
- Gibson reaction of TorA-Cas9:
- 3.3µL water
- 1.16 µL backbone
- 0.54µL insert
- 5µL Gibson Mix
- Miniprep Results:
Jordan & Tyler
- Ran a Gibson on Cas9-Lrz-SS and TorA (with PCR-added homology)
- Made 5 mL overnight cultures of:
- 10 colonies from Cas9-DsbA Gibson assembly product with PCR-added homology to insert
- 2 colonies from Cas9-DsbA Gibson assembly product with original DsbA gBlock
- Gibson reaction of TorA-Cas9:
- 3.3µL water
- 1.16 µL backbone
- 0.54µL insert
- 5µL Gibson Mix
- Miniprep Results:
