Thursday, September 22nd
Tasks:
Jordan
- Checked new overnight culture from the one plate with lots of colonies
- Still didn’t grow much
- Made a new overnight culture from the other plate once the colony is big enough
- Miniprepped pET28a-Cas9 and pET28a-Cas9-SS Gibson cultures (leave 500uL culture for glycerol stocks)
- Accidentally used all of the culture
- Miniprepped pSB1C3-Cas9 ligation cultures (leave 500uL culture for glycerol stocks)
- Accidentally used all of the culture
Tasfia
- Nanodropped minipreps
Jordan
- Checked new overnight culture from the one plate with lots of colonies
- Still didn’t grow much
- Made a new overnight culture from the other plate once the colony is big enough
- Miniprepped pET28a-Cas9 and pET28a-Cas9-SS Gibson cultures (leave 500uL culture for glycerol stocks)
- Accidentally used all of the culture
- Miniprepped pSB1C3-Cas9 ligation cultures (leave 500uL culture for glycerol stocks)
- Accidentally used all of the culture
Tasfia
- Nanodropped minipreps
