Team:OUC-China/Part Collection

Part Collection

We devoted to constructing a library of stem-loops to regulate the expression of protein and exploring a toolkit.
We tested both native stem-loops and designed stem-loops and we found some parts can coordinate the expression well. Therefore, we submitted these parts to iGEM parts registry with detailed characterization. Then, with the demands of bigger library and more detailed data, we collected these measured stem-loops with various folding free energy to work on constructing a toolkit.

Part Number	Type	Description	Designer	Length(bp)
BBa_K2047001	Composite	GFP_Stem-loop with free energy of -25.6 measured by Mfold	Yu Jiang	801
BBa_K2047003	Composite	GFP_Stem-loop with free energy of -38.7measured by Mfold	Yu Jiang	809
BBa_K2047101	Composite	GFP_Stem-loop with free energy of -30.1 measured by Mfold	Yu Jiang	814
BBa_K2047102	Composite	GFP_Stem-loop with free energy of -14.9 measured by Mfold	Yu Jiang	800
BBa_K2047103	Composite	GFP_Stem-loop with free energy of -38.8 measured by Mfold	Yu Jiang	821
BBa_K2047104	Composite	GFP_Stem-loop with free energy of -34.4 measured by Mfold	Yu Jiang	818
BBa_K2047106	Composite	GFP_Stem-loop with free energy of -38.5 measured by Mfold	Yu Jiang	822
BBa_K2047107	Composite	GFP_Stem-loop with free energy of -44.9 measured by Mfold	Yu Jiang	798
BBa_K2047108	Composite	GFP_Stem-loop with free energy of -38.8 measured by Mfold	Yu Jiang	824

Cistrons Concerto

Thanks

1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

2.NEW ENGLAND Biolabs

3.Genscript

Contact us:

E-mail: oucigem@163.com

Designed and built by @ Jasmine Chen and @ Zexin Jiao

We are OUC-iGEM