![](../wiki/images/9/98/T--Shenzhen_SFLS--transparent.png)
Notebook
8.1-8.7
Luciferase expression test: in liquid environment
8.8-8.14
Luciferase expression test: on test paper (liquid)
GFP expression test: on test paper (liquid)
8.15-8.21
Luciferase expression test: in liquid environment
Exploring variables of luciferase: 15ul, dilute into 50ul, then gradually added to the rest of the volume of the assay. And then gradually add 1/2 assay.
8. 23-8.31 Plasmid extraction, test sequence
9.18-9.25 Recovering PAAV-LACZ fragment by PCR and agarose gel electrophoresis Enzyme restricting and adding lacz to plasmid vector
9.25-9.31 Lacz expression test: in liquid environment
10.1-10.2 PCR, preparing for another cycle of plasmid(parts submission) construction
10.3 -10.4 analysing the reason why we get bad gel electrophoresis results
![](../wiki/images/3/30/T--Shenzhen_SFLS--无标题.png)
10.5-10.6 start another round of PCR
10.7-10.8 cut PCR product with restriction enzyme and start ligation reaction
10.9-10.10 transformed and failed due to the low quality of our competent cell
![](../wiki/images/8/88/T--Shenzhen_SFLS--9C34DC0579A9A6C4AC8833F5F5C43247.jpg)
10.11-10.19 start a new round of plasmid construction
![](../wiki/images/0/04/T--Shenzhen_SFLS--P2.png)
![](../wiki/images/3/3a/T--Shenzhen_SFLS--p3.png)
re-transform a former set of plasmids since we need them for our confirmatory experimen
![](../wiki/images/f/f8/T--Shenzhen_SFLS--3BDE401939666BF30D7B732419011D1F.jpg)
![](../wiki/images/c/ce/T--Shenzhen_SFLS--F377147BDFB2418D962CD43D4F5298FE.jpg)
mini-preps
![](../wiki/images/a/af/T--Shenzhen_SFLS--CFB98563C5D9662C2746D282FB200B70.png)
test toehold switches
![](../wiki/images/8/86/T--Shenzhen_SFLS--5DDE24ECCD5C1A8D55E25227280E4BA1.jpg)
![](../wiki/images/1/1a/T--Shenzhen_SFLS--2014placeholderimage.jpg)