Difference between revisions of "Team:Manchester/Safety"
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<img style="width:700px;" src="https://static.igem.org/mediawiki/2016/3/3c/T--Manchester--safety_table.png" alt="safety table"></img> | <img style="width:700px;" src="https://static.igem.org/mediawiki/2016/3/3c/T--Manchester--safety_table.png" alt="safety table"></img> | ||
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| − | <p>EtBr is commonly used in laboratories to identify the band sizes of DNA samples under ultraviolet light after being loaded onto agarose gels. | + | <p style="font-size:17px">EtBr is commonly used in laboratories to identify the band sizes of DNA samples under ultraviolet light after being loaded onto agarose gels. |
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| − | <p>Similar to EtBr, SYBR Safe enables the visualization of nucleic acid bands using agarose gels. In comparison, SYBR Safe has been identified to be 4 to 5 times less mutagenic than EtBr <sup>[4]</sup>. Thus, we substituted EtBr with SYBR Safe for most of our nucleic acid staining procedures. | + | <p style="font-size:17px">Similar to EtBr, SYBR Safe enables the visualization of nucleic acid bands using agarose gels. In comparison, SYBR Safe has been identified to be 4 to 5 times less mutagenic than EtBr <sup>[4]</sup>. Thus, we substituted EtBr with SYBR Safe for most of our nucleic acid staining procedures. |
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| − | <p>ABTS is widely used to detect the binding activity of molecules in presence of a peroxidase enzyme (eg: HRP). | + | <p style="font-size:17px">ABTS is widely used to detect the binding activity of molecules in presence of a peroxidase enzyme (eg: HRP). |
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| − | <p>GOx catalyzes the oxidation of glucose to hydrogen peroxide (H2O2) and D-glucono-δ-lactone. GOx is used in our <a src="">pilot experiment </a> to justify our proof of concept before we kick start our <a src="">actual project </a> with alcohol oxidase (AOx). | + | <p style="font-size:17px">GOx catalyzes the oxidation of glucose to hydrogen peroxide (H2O2) and D-glucono-δ-lactone. GOx is used in our <a src="">pilot experiment </a> to justify our proof of concept before we kick start our <a src="">actual project </a> with alcohol oxidase (AOx). |
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HRP catalyzes the redox reaction and amplify the signals of chromogenic (eg: ABTS) and chemiluminescence (eg: luminol) substrates. The reaction is fast and visible, as shown in our <a src="">pilot study </a>. | HRP catalyzes the redox reaction and amplify the signals of chromogenic (eg: ABTS) and chemiluminescence (eg: luminol) substrates. The reaction is fast and visible, as shown in our <a src="">pilot study </a>. | ||
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Revision as of 22:34, 19 September 2016
Safety is absolutely paramount in the scientific research area. In conducting scientific experiments, researchers should always comply to the safety measurements outlined in the Health and Safety at Work Act 1974, c.37 [1], not only to protect themselves, but also the members of the public and environment from any potential harm.
Scroll to check out the safety measurements we took!
Ethidium Bromide (EtBr)
- Hazard statements: H302, H330, H341
- Risks: Harmful if swallowed and inhaled, highly mutagenic and may be carcinogenic [3].
EtBr is commonly used in laboratories to identify the band sizes of DNA samples under ultraviolet light after being loaded onto agarose gels.
Usage of EtBr has been reduced in our lab by using SYBR Safe as a substitute to minimize the risks as stated above.
SYBR Safe
- Hazard statements: H227
- Risks: Flammable, may cause mild eye and skin irritation
Similar to EtBr, SYBR Safe enables the visualization of nucleic acid bands using agarose gels. In comparison, SYBR Safe has been identified to be 4 to 5 times less mutagenic than EtBr [4]. Thus, we substituted EtBr with SYBR Safe for most of our nucleic acid staining procedures.
ABTS
(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid))
- Hazards statements: H315, H319, H335
- Risks: May cause skin, eye and respiratory irritation.
ABTS is widely used to detect the binding activity of molecules in presence of a peroxidase enzyme (eg: HRP).
The possibility of causing skin irritation when in direct contact with ABTS indicates that ABTS may not be the best candidate for our actual AlcoPatch. For future experiments, ABTS should be substituted with other safer redox indicators.
Glucose Oxidase (GOx)
- Hazards statements: H334
- Risks: May cause skin and respiratory irritation.
GOx catalyzes the oxidation of glucose to hydrogen peroxide (H2O2) and D-glucono-δ-lactone. GOx is used in our pilot experiment to justify our proof of concept before we kick start our actual project with alcohol oxidase (AOx).
Horseradish Peroxidase (HRP)
- Hazards statements: H334, H317
- Risks: May cause skin and respiratory irritation.
HRP catalyzes the redox reaction and amplify the signals of chromogenic (eg: ABTS) and chemiluminescence (eg: luminol) substrates. The reaction is fast and visible, as shown in our pilot study .
- Design a kill switch system to minimize that the risk of releasing live E.coli organisms to the environment.
- Explore other redox indicators as alternatives for ABTS.
