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− | [[File:MarA plate assay | + | [[File:MarA plate assay kamata did.png|750px|thumb|left|Fig. 13: Colony formation efficiencies of ''E. coli'' JM109 engineered with ''marA'' on geraniol overlaid plates. |
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''E. coli'' JM109 and ''E. coli'' JM109 (''marA'') were spotted on LBGMg agar plates in serial ten-fold dilutions (10⁻¹~10⁻6), overlaid with 30.0 % (v/v) fanesol hexane solution (farnesol solution), and incubated at 30°C for 24 h. This figure shows that ''E. coli'' JM109 (''marA'') cells that overexpress the ''marA'' product is more survived on 30.0 % farnesol solution overlay plates than the counterpart control ''E. coli'' JM109 wild type cells. | ''E. coli'' JM109 and ''E. coli'' JM109 (''marA'') were spotted on LBGMg agar plates in serial ten-fold dilutions (10⁻¹~10⁻6), overlaid with 30.0 % (v/v) fanesol hexane solution (farnesol solution), and incubated at 30°C for 24 h. This figure shows that ''E. coli'' JM109 (''marA'') cells that overexpress the ''marA'' product is more survived on 30.0 % farnesol solution overlay plates than the counterpart control ''E. coli'' JM109 wild type cells. |
Revision as of 10:59, 22 September 2016
Result
Enhancement of farnesol resistance
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