Difference between revisions of "Team:Manchester/Project/Protocols"

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   <img style="width:100%; margin-bottom:100px;margin-top:50px;" src="https://static.igem.org/mediawiki/2016/a/a5/T--Manchester--protocolsbanner.png" alt="Protocols banner" />
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   <img class="protocolbanner" src="https://static.igem.org/mediawiki/2016/a/a5/T--Manchester--protocolsbanner.png" alt="Protocols banner" />
  
 
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<div class="column full_size">
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   <img src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--protocols_bigtitle.png" alt="symbol" style="width:150px;float:left" />
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   <img src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--protocols_bigtitle.png" alt="symbol" style="width:150px;float:left;" />
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   <h1 style="font-size:30px;color:orange;float:left;height:100px;line-height:100px;vertical-align:middle;">Medium and Buffers</h1>
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   <h1 class="sub_title">Medium and Buffers</h1>
 
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   <h2 style="font-size:20px;color:rgb(197,90,17);padding:10px;">20% (w/v) glucose solution </h2>
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   <h2 class="sub_sub_title">20% (w/v) glucose solution </h2>
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  <br />
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    <br />
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<!--
 
   <p style="padding-left:10px;padding-bottom:0px"><i>per 1L</i></p>
 
   <p style="padding-left:10px;padding-bottom:0px"><i>per 1L</i></p>
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   <table style="border:solid 1px black;border-collapse:collapse;" class="protocols_table">
 
   <table style="border:solid 1px black;border-collapse:collapse;" class="protocols_table">
 
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     <tr>
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  <img src="https://static.igem.org/mediawiki/2016/2/2f/T--Manchester--protocoltable1.png" alt="table 1" />
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    <br />
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  <ol>
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    <li> Prepare the solution by mixing the ingredients stated above. </li>
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    <li> Sterilize in an autoclave before using it to prepare the SOC medium.</li>
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  </ol>
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  <br />
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  <h2 class="sub_sub_title">50% (w/v) glycerol solution</h2>
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    <br />
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  <img src="https://static.igem.org/mediawiki/2016/6/66/T--Manchester--protocoltable2.png" alt="table2" />
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    <br />
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  <ol >
 +
    <li>Prepare the solution by mixing the ingredients stated above. </li>
 +
    <li>Sterilize in an autoclave.</li>
 +
  </ol>
 +
    <br />
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  <p>*Source from 2015 iGEM Exeter [link = 2015.igem2015 .org/wiki/images/a/ab/Exeter_Glycerol_recipe.pdf]</p>
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    <br />
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  <h2 class="sub_sub_title">Electrophoresis buffers</h2>
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    <br />
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  <p class="font20"><i>For nucleic acid DNA/RNA separation</i></p>
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  <ul>
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    <li class="sub_sub_title1">TAE buffer (Tris-acetate-EDTA)</li>
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    <li class="sub_sub_title1">TBE buffer (Tris-borate-EDTA)</li>
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    <li class="sub_sub_title1">LB buffer (Lithium borate) </li>
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  </ul>
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    <br />
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  <img src="https://static.igem.org/mediawiki/2016/d/da/T--Manchester--protocoltable3.png" alt="table3" />
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    <br />
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  <ol>
 +
    <li>Prepare the solution by mixing the ingredients stated above. </li>
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  </ol>
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    <br />
 +
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  <h2 class="sub_sub_title">LB agar (Luria-Bertani agar)</h2>
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    <br />
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  <p style="font-size:20px;">If not using pre-mixed LB agar powder, prepare the materials as below:</p>
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    <br />
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  <img src="https://static.igem.org/mediawiki/2016/b/b3/T--Manchester--protocoltable4.png" alt="table4" />
  
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    <br />
 
    
 
    
 
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Revision as of 10:14, 23 September 2016

Manchester iGEM 2016

Protocols banner

symbol

Medium and Buffers

20% (w/v) glucose solution


table 1
  1. Prepare the solution by mixing the ingredients stated above.
  2. Sterilize in an autoclave before using it to prepare the SOC medium.

50% (w/v) glycerol solution


table2
  1. Prepare the solution by mixing the ingredients stated above.
  2. Sterilize in an autoclave.

*Source from 2015 iGEM Exeter [link = 2015.igem2015 .org/wiki/images/a/ab/Exeter_Glycerol_recipe.pdf]


Electrophoresis buffers


For nucleic acid DNA/RNA separation

  • TAE buffer (Tris-acetate-EDTA)
  • TBE buffer (Tris-borate-EDTA)
  • LB buffer (Lithium borate)

table3
  1. Prepare the solution by mixing the ingredients stated above.

LB agar (Luria-Bertani agar)


If not using pre-mixed LB agar powder, prepare the materials as below:


table4

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