Line 5: | Line 5: | ||
<style> | <style> | ||
+ | .divindiv{ | ||
+ | margin:auto; | ||
+ | } | ||
+ | |||
.agarinfo, .electrophoresisinfo, .glucoseinfo, .glycerolinfo{ | .agarinfo, .electrophoresisinfo, .glucoseinfo, .glycerolinfo{ | ||
display:none; | display:none; | ||
Line 16: | Line 20: | ||
color: white; | color: white; | ||
background-color: rgb(197,90,17); | background-color: rgb(197,90,17); | ||
+ | border-radius:10px 10px 0px 0px | ||
} | } | ||
Line 86: | Line 91: | ||
<div class="column full_size"> | <div class="column full_size"> | ||
+ | <div class="divindiv" style="margin:auto"> | ||
<img src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--protocols_bigtitle.png" alt="symbol" style="width:150px;float:left;" /> | <img src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--protocols_bigtitle.png" alt="symbol" style="width:150px;float:left;" /> | ||
<h1 class="sub_title">Medium and Buffers</h1> | <h1 class="sub_title">Medium and Buffers</h1> | ||
+ | </div> | ||
</div> | </div> | ||
Revision as of 19:57, 23 September 2016
Medium and Buffers
- Prepare the solution by mixing the ingredients stated above.
- Sterilize in an autoclave before using it to prepare the SOC medium.
- Prepare the solution by mixing the ingredients stated above.
- Sterilize in an autoclave.
*Source from 2015 iGEM Exeter [link = 2015.igem2015 .org/wiki/images/a/ab/Exeter_Glycerol_recipe.pdf]
For nucleic acid DNA/RNA separation
- TAE buffer (Tris-acetate-EDTA)
- TBE buffer (Tris-borate-EDTA)
- LB buffer (Lithium borate)
- Prepare the solution by mixing the ingredients stated above.
If not using pre-mixed LB agar powder, prepare the materials as below:
- In a 1L Erlenmeyer flask, swirl and mix the solution.
- Cover the top of the flask with a lid/aluminum foil and label with autoclave tape.
- Autoclave the liquid setting for 20 minutes or according to your autoclave's specifications.
- After removing the solution from the autoclave, allow the agar solution to cool to 55°C in an oven or water bath.
- When pouring the LB agar into plates, keep the bench area sterile by working near a flame or Bunsen burner. Alternatively, prepare the plates in a vacuum hood.
- Add the appropriate amount of desired antibiotic (refer the table below) to the solution and swirl to mix.
- Pour approximately 20mL of LB agar per 10cm polystyrene Petri dish.
- Place the lids on the plates and allow them to cool for until the agar is solidified.
- Label the bottom of plates with antibiotic and date before storing in plastic bags or sealed with Parafilm at 4°C.