Difference between revisions of "Team:Emory/test"

 
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{{Team:Emory/Test}}
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<html lang="en">
 
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+
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         -webkit-background-size: cover;
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background-size: cover;
 
background-size: cover;
 
background-attachment: fixed;
 
background-attachment: fixed;
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}
 
}
  
 
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background: #EDE2DF;
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#what-we-do {
 
       background: white;
 
       background: white;
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}
 
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#divison-one {
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    background: white;
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    height: 50px;
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background-size: cover;
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background-attachment: fixed;
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#divison-four {
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    background-color: white;
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    height: 50px;
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background-size: cover;
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background-attachment: fixed;
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display: inline;
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margin: 15px;
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button.accordion.active, button.accordion:hover {
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div.panel {
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div.panel.show {
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</style>  
 
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<script>
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//Smooth Scroll Dammit
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$(document).ready(function(){
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// Toggle between adding and removing the "active" and "show" classes when the user clicks on one of the "Section" buttons. //The "active" class is used to add a background color to the current button when its belonging panel is open. The "show" //class is used to open the specific accordion panel
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</head>
 
</head>
−
<body>
+
 
 +
 
 +
<body>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<!-- NAV BAR-->
 +
<nav class="navbar navbar-inverse navbar-fixed-top">
 +
  <div class="container-fluid">
 +
    <div class="navbar-header">
 +
      <a class="navbar-brand" href="#"></a>
 +
    </div>
 +
    <ul class="nav navbar-nav">
 +
      <li class="active"><a href="#welcome-page" class="smoothScroll">Home</a></li>
 +
      <li><a href="#explore" class="smoothScroll">Map</a></li>
 +
      <li><a href="#what-we-do" class="smoothScroll">Purpose</a></li>
 +
      <li><a href="#project" class="smoothScroll">Project</a></li>
 +
      <li><a href="#notebook" class="smoothScroll">Notebook</a></li>
 +
      <li><a href="#parts" class="smoothScroll">Parts</a></li>
 +
      <li><a href="#team" class="smoothScroll">Meet the Team</a></li>
 +
      <li><a href="#judge" class="smoothScroll">Judging and Evaluations</a></li>
 +
    </ul>
 +
  </div>
 +
</nav>
 
 
 
 
 
<!-- PAGE 1 WELCOME PAGE -->
 
<!-- PAGE 1 WELCOME PAGE -->
  
−
<div id="Welcome-Page" style="text-align:center">
+
<div id="welcome-page" style="text-align:center">
 
   <div class="about" class="smooth"></a>
 
   <div class="about" class="smooth"></a>
 
     <div class= "container">
 
     <div class= "container">
−
         <h1 style="text-align:center;font-size:60px;color:white;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:250px;"><strong>EMORY BIOTECH</strong></h1>
+
         <h1 style="text-align:center;font-size:60px;color:white;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:275px;"><strong>EMORY BIOTECH</strong></h1>
−
         <p style="text-align:center;font-size:40px;color:white;">"to create, preserve, teach, and apply knowledge in service of humanity"</p>
+
         <p style="font-size:40px;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;text-align:center;color:white;padding-bottom:500px;">"to create, preserve, teach, and apply knowledge in service of humanity"</p>
−
</div>
+
  
 +
 +
</div>
  
  
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  <!-- PAGE 2 EXPLORE -->
 
  <!-- PAGE 2 EXPLORE -->
 
   
 
   
−
<div id="Explore">
+
<div id="explore">
 
   <div class="container">
 
   <div class="container">
−
     <h1 style="text-align:center;font-size:50px;color:343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:250px;"><strong>EXPLORE</strong></h1>
+
     <h1 style="text-align:center;font-size:50px;color:#343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;"><strong>EXPLORE</strong></h1>
−
     <p style="text-align: center; font-size:24px;color:#828282;"><i>Our Wiki page is organized in a comprehensive, linear manner so that every user can navigate it easily.Want to skip ahead a few sections?  
+
     <p style="text-align:center;font-size:18px;color:#343738;padding-bottom:50px;"><i>Our Wiki page is organized in a comprehensive, linear manner so that every user can navigate it easily. Want to skip ahead a few sections?  
−
Have a go at the navigation bar and it'll push you in the right direction. Other than that, lean back and let us guide you. You can always use the navigation bar to bring you back to our core pages, including this small
+
Have a go at the navigation bar and it'll push you in the right direction. If you ever get lost, you can always use it to return to our core pages, including the more detailed map below. I don't know what I just typed, I just needed to see what the text looks like. All of this will be edited further later.</i></p>
−
map. I don't know what I just typed I just need to see what the text looks like. All of this will change later.</i></p>
+
  
−
<div class="guide-button" id ="guide-button">
 
−
<button type="button" class="btn btn-default btn-block">Our Purpose</button>
 
−
<button type="button" class="btn btn-default btn-block">Meet the Team</button>
 
−
<button type="button" class="btn btn-default btn-block">Human Practices & Outreach</button>
 
−
<button type="button" class="btn btn-default btn-block">Project</button>
 
−
<button type="button" class="btn btn-default btn-block">Parts</button>
 
−
<button type="button" class="btn btn-default btn-block">Safety</button>
 
−
<button type="button" class="btn btn-default btn-block">Support</button>
 
  
−
</div>
 
 
</div>
 
</div>
 
</div>
 
</div>
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<div id="what-we-do">
 
<div id="what-we-do">
 
   <div class="container">
 
   <div class="container">
−
     <h1 style="text-align:center;font-size:50px;color:343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:250px;">What Are We Doing Here?</h1>
+
     <h1 style="text-align:center;font-size:50px;color:#343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;">WHAT ARE WE DOING HERE?</h1>
−
     <p>Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.</p>
+
     <p style="font-size:18px;">Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.</p>
  
 +
<img src="https://static.igem.org/mediawiki/2016/f/fb/TeamEmory_Koli.png" alt="Coli" style="width:562x;height:450px;">
 +
  <img src="https://static.igem.org/mediawiki/2016/2/21/TeamEmory_ADP1Ex.png" alt="ADP1" align="right;">
 +
</div>
 
</div>
 
</div>
−
 
  
  
 
<!--PAGE 4 PROJECT, DESCRIPTION, DESIGN, RESULTS, NOTEBOOK -->
 
<!--PAGE 4 PROJECT, DESCRIPTION, DESIGN, RESULTS, NOTEBOOK -->
−
<div id="Project">
+
<div id="project">
 
   <div class="container">
 
   <div class="container">
−
      <h1>Description</h1>
+
    <h1 style="text-align:center;font-size:50px;color:#343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;">PROJECT</h1>
−
      <p>Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries.
+
−
One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation
+
−
of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle
+
−
vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that
+
−
A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by
+
−
spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin).
+
−
These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and
+
−
even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and
+
−
capable of replication in the widely used E. coli chassis.</p>
+
  
−
       <h1>Design</h1>
+
       <h2 style="text-align:left;">Description</h2>  
−
        <h3>-Research-</h3>
+
 
−
<p>Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries.  
+
      <img src="https://static.igem.org/mediawiki/2016/0/03/TeamEmory_DahliaEx.png" alt="flower1" style="float:right;">
−
One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation  
+
 
−
of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle
+
      <p style="text-align:left;padding-bottom:70px;">Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttlevectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.</p>
−
vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that  
+
 
−
A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by  
+
      <h2 style="text-align:right;">Design, Protocol, and Experiments</h2>
−
spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin).  
+
 
−
These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and  
+
      <img src="https://static.igem.org/mediawiki/2016/3/35/TeamEm_WetLeafEx.png" alt="leaf" style="float:left;width:510px;height:330px;">
−
even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and  
+
 
−
capable of replication in the widely used E. coli chassis.</p>
+
      <p style="text-align:right;padding-bottom:70px;">Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttlevectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges.</p>
−
<h3>-Protocol-</h3>
+
−
<p>Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries.  
+
−
One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation
+
−
of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle
+
−
vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that
+
−
A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by
+
−
spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin).
+
−
These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and
+
−
even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and
+
−
capable of replication in the widely used E. coli chassis.</p>
+
−
<h3>-Experiments-</h3>
+
−
<p>Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries.  
+
−
One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation  
+
−
of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle
+
−
vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that  
+
−
A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by  
+
−
spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin).  
+
−
These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and
+
−
even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and
+
−
capable of replication in the widely used E. coli chassis.</p>
+
−
+
 
 
−
<h1>Results</h1>
+
    <h2 style="text-align:left;">Results</h2>
−
<p>Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries.  
+
     
−
One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation
+
      <img src="https://static.igem.org/mediawiki/2016/b/b6/TeamEmory_PurpleFlowerEx.png" alt="flower2" style="float:right;width:359px;height:332px;">
−
of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle
+
 
−
vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that
+
    <p style="text-align:left;padding-bottom:70px;">Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttlevectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.</p>
−
A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by
+
−
spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin).
+
 
−
These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and
+
</div>
−
even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and
+
−
capable of replication in the widely used E. coli chassis.</p>
+
−
<h1>Notebook</h1>
+
−
<p>Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries.  
+
−
One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation  
+
−
of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle
+
−
vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that  
+
−
A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by  
+
−
spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin).  
+
−
These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and  
+
−
even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and  
+
−
capable of replication in the widely used E. coli chassis.</p>
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</div>
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</div>
 
</div>
  
  
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<!--PAGE 5 NOTEBOOK -->
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<div id="notebook">
 +
  <div class="container">
 +
    <h1 style="text-align:center;font-size:50px;color:#343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;">NOTEBOOK</h1>
 +
<p style="padding-bottom: 50px;">We've recorded all of our research achievements and blunders along the way in an online notebook, so you can experience our excitement, thoughts, and the challenges we've encountered and overcome throughout this process.</p>
  
 +
    </div>
 +
</div>
  
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<!--PARTS-->
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 +
<!--ACCORDION IN NOTEBOOK-->
 +
 
 +
<button class="accordion">WEEK ONE</button>
 +
<div class="panel">
 +
  <p>things happened</p>
 +
</div>
 +
 
 +
<button class="accordion">WEEK TWO</button>
 +
<div class="panel">
 +
  <p>things happened</p>
 +
</div>
 +
 
 +
<button class="accordion">WEEK THREE</button>
 +
<div class="panel">
 +
  <p>things happened</p>
 +
</div>
 +
 
 +
 
 +
 
 +
<!--PAGE 6 PARTS-->
 
   <div class="parts" id="parts">
 
   <div class="parts" id="parts">
 
             <div class="container">
 
             <div class="container">
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               <h1 style="text-align:center;font-size:50px;color:343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:250px;"><strong>PARTS</strong></h1>
+
               <h1 style="text-align:center;font-size:50px;color:#343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;"><strong>PARTS</strong></h1>
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</div>
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    </div>
 
</div>
 
</div>
  
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         <td>Part 1</td>
 
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         <td>Type 1</td>
 
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         <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.
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         <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
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This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.
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This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
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<td>Length 1</td>
 
<td>Length 1</td>
 
       </tr>
 
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         <td>Type 1</td>
 
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         <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.
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         <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
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This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.
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This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
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<td>Length 1</td>
 
<td>Length 1</td>
 
       </tr>
 
       </tr>
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         <td>Part 1</td>
 
         <td>Type 1</td>
 
         <td>Type 1</td>
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         <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.
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         <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
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This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.
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This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
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<td>Length 1</td>
 
<td>Length 1</td>
 
       </tr>
 
       </tr>
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        <td>Part 1</td>
 
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         <td>Type 1</td>
 
         <td>Type 1</td>
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         <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.
+
         <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
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This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.
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<td>Length 1</td>
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This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
+
  </tr>
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  <tr>
 +
        <td>Part 1</td>
 +
        <td>Type 1</td>
 +
        <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
 +
<td>Length 1</td>
 +
  </tr>
 +
  <tr>
 +
        <td>Part 1</td>
 +
        <td>Type 1</td>
 +
        <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
 +
<td>Length 1</td>
 +
  </tr>
 +
  <tr>
 +
        <td>Part 1</td>
 +
        <td>Type 1</td>
 +
        <td>This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.</td>
 
<td>Length 1</td>
 
<td>Length 1</td>
 
  </tr>
 
  </tr>
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</div>
 
</div>
  
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</div>
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</div>
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</div>
+
  
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+
<!--PAGE 7 SAFETY-->
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<!--SAFETY-->
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   <div class="safety" id="safety">
 
   <div class="safety" id="safety">
 
             <div class="container">
 
             <div class="container">
−
               <h1 style="text-align:center;font-size:50px;color:343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:250px;"><strong>SAFETY</strong></h1>
+
               <h1 style="text-align:center;font-size:50px;color:color:#343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;"><strong>SAFETY</strong></h1>
 +
              <h2>Our Concerns</h2>
  
−
  </div>
+
              <p style="text-align:center">Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.</p>
 +
 
 +
              <h2>How We Addressed Them</h2>
 +
              <p style="text-align:center">Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.</p>
 +
</div>
 
</div>
 
</div>
 +
 +
 +
<!--DIVISION THREE-->
 +
<div class="division-three" id="division-three">
 +
  <div class="container">
 +
  <p style="color:white;"> </p>
 +
</div>
 +
</div>
 +
 +
<!--PAGE 8 HUMAN PRACTICES-->
 +
 +
  <div class="human-practices" id="human-practices">
 +
            <div class="container">
 +
              <h1 style="text-align:center;font-size:50px;color:black;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;"><strong>HUMAN PRACTICES & OUTREACH</strong></h1>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/f/f6/TeamEmory_THelper.png" alt="guide" style="width:600px;height:600px;">
 +
 +
 +
              <p style="font-size:18px;">Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.</p>
 +
 +
              <p style="padding-bottom:50px;font-size:18px;">Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.</p>
 +
 +
    </div>
 +
</div>
 +
 +
<!--PAGE 9 TEAM-->
 +
 +
  <div class="team" id="team">
 +
            <div class="container">
 +
              <h1 style="text-align:center;font-size:50px;color:white;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;"><strong>TEAM</strong></h1>
 +
              <div class="row">
 +
                <div class="clearfix">
 +
 +
</div>
 +
               
 +
 +
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 1" />
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>JASMINE CARROTHERS</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
                        <li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 2" />
 +
                                </div>
 +
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>CYRILLUS TAN</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Secondary PI</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
                        <li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>TALIA AKOH-ARREY</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
 +
                        <li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 4" />
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>MONE ANZAI</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
</li>
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 4" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>KATHY LI</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>SUJITH SWARNA</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>HYUN HWANG</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>ROHITA MOUDGAL</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>JOSH MIRAGLIA</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>KRISHNA MARISETTI</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>Munsa Manandahar</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
 +
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>MARUF M. HOQUE</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
<li class="col-lg-3 col-md-3 col-sm-6 ">
 +
                            <div class="text-center">
 +
                                <div class="member-thumb">
 +
                                    <img src="https://static.igem.org/mediawiki/2016/1/11/IGemEmoryXFiles.jpeg" class="img-responsive" alt="member 3" />
 +
 +
                                </div>
 +
                                <div class="team-inner">
 +
                                    <p class="team-inner-header"><span style="color:white;"><strong>DR. ICHIRO MATSUMURA</p></span></strong>
 +
                                    <p class="team-inner-subtext"><span style="color:white;">Student</p></span>
 +
                                </div>
 +
                            </div>
 +
                        </li>
 +
 +
 +
 +
 +
 +
 +
</ul>
 +
</div>
 +
</div>
 +
 +
 +
<!--PAGE 10-->
 +
  <div class="attributions" id="attributions">
 +
            <div class="container">
 +
              <h1 style="text-align:center;font-size:50px;color:343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif;padding-top:150px;"><strong>ATTRIBUTIONS</strong></h1>
 +
              <p style="font-size:18;">We've come a long way and it's time to give credit where it's due.</p>
 +
 +
 +
<h2 style="text-align:center;">Building and Establishing Our Team</h2>
 +
  <p style="font-size:13;">Emory Biotech was created by <span style="color:#22A161;">Rohita</span> and <span style="color:#22A161;">Josh</span> (and other people whose names I will need). We'll add a few more lines to give proper thanks to these individuals for taking the time to establish a base from which this group could flourish (funding from department, legitimizing the club..etc). Note: Most of the text in these sections are just to show what attributions will look like. I will definitely add more categories to make sure all aspects of this project ae covered and people get due credit</p>
 +
 +
 +
<h2 style="text-align:center;">Experimental Design and Conduct</h2>
 +
  <p style="font-size:13;">Some <strong><span style="color:#22A161;">student(s)</strong></style> was responsible for this and this and that and that. Someone came up with the experimental design and other things with the help of <strong><span style="color:#22A161;">Dr. Matsumura</strong></span>...etc.</p>
 +
 +
<h2 style="text-align:center;">General Lab Support/Challenging Technique</h2>
 +
  <p style="font-size:13;"><span style="color:#22A161;">Dr. Matsumura</span> generously provided his lab space and plenty of his time in teaching our researchers important lab techniques....etc.</p
 +
>
 +
<h2 style="text-align:center;">KickStarter Campaign Management</h2>
 +
  <p style="font-size:13;"><strong><span style="color:#22A161;">Maruf</span></strong> did many things etc etc etc.</p>
 +
 +
<h2 style="text-align:center;">Human Practices and Outreach Coordinator</h2>
 +
  <p style="font-size:13;"><strong><span style="color:#22A161;">Someone</span></strong> did many things to make this possible etc etc etc.</p>
 +
<h2 style="text-align:center;">Lead Wiki and Graphic Designer</h2>
 +
  <p style="font-size:13;">The wiki was designed, supervised, and maintained by <span style="color:#22A161;"><strong>Talia</strong></span>. Logos were done by...etc.</p>
 +
 +
<p>General Support, Project support and advice, Fundraising help and advice, Lab support, Difficult technique support, Project advisor support, Wiki support, Presentation coaching, Human Practices support, Thanks and acknowledgements for all other people involved in helping make a successful iGEM team. </p>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
<!--PAGE 12 JUDGING PAGES-->
 +
 +
  <div class="judge" id="judge">
 +
            <div class="container">
 +
              <h1 style="text-align:center;font-size:50px;color:#343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif; padding-top:150px;padding-bottom:50px;"><strong>JUDGING AND EVALUATIONS</strong></h1>
 +
    <p style="text-align:center;font-size:18px;color:#343738;padding-bottom:50px;">For the convenience of the judges, we've gathered all the links to the specific pages we'd like to be evaluated for and put them in one spot.</p>
 +
 +
<div class="guide-button" id ="guide-button">
 +
<a href="https://2016.igem.org/Team:Emory/Description"button type="button" class="btn btn-default btn-block">Project Description</button></a>
 +
<a href="https://2016.igem.org/Team:Emory/Design"button type="button" class="btn btn-default btn-block">Project Design</button></a>
 +
<a href="https://2016.igem.org/Team:Emory/Design"button type="button" class="btn btn-default btn-block">Proof of Concept</button></a>
 +
<a href="https://2016.igem.org/Team:Emory/Demonstrate"button type="button" class="btn btn-default btn-block">Demonstration And Results</button></a>
 +
<a href="https://2016.igem.org/Team:Example/Notebook"button type="button" class="btn btn-default btn-block">Notebook</button></a>
 +
<a href="https://2016.igem.org/Team:Emory/Parts"button type="button" class="btn btn-default btn-block">Parts</button></a>
 +
<a href="https://2016.igem.org/Team:Emory/Integrated_Practices"button type="button" class="btn btn-default btn-block">Integrated Human Practices</button></a>
 +
<a href="https://2016.igem.org/Team:Emory/Engagement" button type="button" class="btn btn-default btn-block">Education and Public Engagement</button></a>
 +
<a href="https://2016.igem.org/Team:Emory/Attributions"button type="button" class="btn btn-default btn-block">Attributions</button></a>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<!--PAGE 12 SPONSORS-->
 +
  <div class="sponsors" id="sponsors">
 +
            <div class="container">
 +
              <h1 style="text-align:center;font-size:50px;color:#343738;font-family:Open Sans, Arial, Helvetice Neue, sans-serif; padding-top:150px;padding-bottom:50px;"><strong>SPONSORS</strong></h1>
 +
 +
                  <img src="https://static.igem.org/mediawiki/2016/a/a3/TeamEmoryIDTLogo.png" class="sponsor_images" />
 +
                  <img src="https://static.igem.org/mediawiki/2016/a/aa/EmoryBiologyLogo.gif" class="sponsor_images" />
 +
                  <img src="https://static.igem.org/mediawiki/2016/5/58/TeamEmoryDepartmentofChemistry.png" class="sponsor_images" />
 +
 +
 +
</div>
 +
</div>
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</body>

Latest revision as of 20:27, 23 September 2016

Team:Emory iGEM 2016

EMORY BIOTECH

"to create, preserve, teach, and apply knowledge in service of humanity"

EXPLORE

Our Wiki page is organized in a comprehensive, linear manner so that every user can navigate it easily. Want to skip ahead a few sections? Have a go at the navigation bar and it'll push you in the right direction. If you ever get lost, you can always use it to return to our core pages, including the more detailed map below. I don't know what I just typed, I just needed to see what the text looks like. All of this will be edited further later.

WHAT ARE WE DOING HERE?

Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.

Coli ADP1

PROJECT

Description

flower1

Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttlevectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.

Design, Protocol, and Experiments

leaf

Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttlevectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges.

Results

flower2

Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttlevectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.

NOTEBOOK

We've recorded all of our research achievements and blunders along the way in an online notebook, so you can experience our excitement, thoughts, and the challenges we've encountered and overcome throughout this process.

things happened

things happened

things happened

PARTS

Name Type Description Length
Part 1 Type 1 This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table. Length 1
Part 1 Type 1 This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table. Length 1
Part 1 Type 1 This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table. Length 1
Part 1 Type 1 This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table. Length 1
Part 1 Type 1 This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table. Length 1
Part 1 Type 1 This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table. Length 1
Part 1 Type 1 This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table.This is a nice part. It has a nice description, I'm sure. Typing a lot of text to see how long the description can be without messing up the table. Length 1

SAFETY

Our Concerns

Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.

How We Addressed Them

Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.

HUMAN PRACTICES & OUTREACH

guide

Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.

Synthetic Biology is largely restricted to well-funded laboratories at major research universities in high income countries. One significant barrier to entry is the capital cost of instruments. The cloning and assembly of BioBricks, for example, includes the transformation of Escherichia coli, which requires the purchase of a refrigerated centrifuge and an ultra-cold freezer. Here we assemble BioBrick-compatible shuttle vectors for Acinetobacter baylyi ADP1, a naturally competent relative of E. coli that grows as rapidly under identical conditions. We will show that A. baylyi can be transformed with recombinant DNA simply by adding ligation reactions to mid-log cultures; transformants are selected as usual by spreading them onto LB agar plates supplemented with the appropriate antibiotics (kanamycin, spectinomycin, tetracycline, cefotaxime or amikacin). These experiments will show how BioBricks can be constructed and assembled in modestly funded laboratories in community colleges, high schools and even private homes. The resulting plasmid constructs retain their pSB1C3 backbones and will thus remain compatible with the BioBrick standard and capable of replication in the widely used E. coli chassis.

TEAM

  • member 1

    JASMINE CARROTHERS

    Student

  • member 2

    CYRILLUS TAN

    Secondary PI

  • member 3

    TALIA AKOH-ARREY

    Student

  • member 4

    MONE ANZAI

    Student

  • member 4

    KATHY LI

    Student

  • member 3

    SUJITH SWARNA

    Student

  • member 3

    HYUN HWANG

    Student

  • member 3

    ROHITA MOUDGAL

    Student

  • member 3

    JOSH MIRAGLIA

    Student

  • member 3

    KRISHNA MARISETTI

    Student

  • member 3

    Munsa Manandahar

    Student

  • member 3

    MARUF M. HOQUE

    Student

  • member 3

    DR. ICHIRO MATSUMURA

    Student

    • ATTRIBUTIONS

    We've come a long way and it's time to give credit where it's due.

    Building and Establishing Our Team

    Emory Biotech was created by Rohita and Josh (and other people whose names I will need). We'll add a few more lines to give proper thanks to these individuals for taking the time to establish a base from which this group could flourish (funding from department, legitimizing the club..etc). Note: Most of the text in these sections are just to show what attributions will look like. I will definitely add more categories to make sure all aspects of this project ae covered and people get due credit

    Experimental Design and Conduct

    Some student(s) was responsible for this and this and that and that. Someone came up with the experimental design and other things with the help of Dr. Matsumura...etc.

    General Lab Support/Challenging Technique

    Dr. Matsumura generously provided his lab space and plenty of his time in teaching our researchers important lab techniques....etc.

    KickStarter Campaign Management

    Maruf did many things etc etc etc.

    Human Practices and Outreach Coordinator

    Someone did many things to make this possible etc etc etc.

    Lead Wiki and Graphic Designer

    The wiki was designed, supervised, and maintained by Talia. Logos were done by...etc.

    General Support, Project support and advice, Fundraising help and advice, Lab support, Difficult technique support, Project advisor support, Wiki support, Presentation coaching, Human Practices support, Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.

    JUDGING AND EVALUATIONS

    For the convenience of the judges, we've gathered all the links to the specific pages we'd like to be evaluated for and put them in one spot.

    SPONSORS