Katzen liegen nicht faul rum. Sie verschönern den Raum.

15/12/15

We had our first team meeting and started with introducing us to each other. To get an overview over our background, every team member shortly described their bachelor topics. We also had a discussion on time requirements and decided that team members should only take one core module during the summer term. Furthermore, it was suggested to organize lab work in shifts. We also exchanged first ideas for possible projects and organized a meeting with Professor Stülke who wanted to present a possible project.

15/12/21

Professor Stülke and members of his lab shortly introduced a possible project from his department based on the idea of a minimal organism. After the meeting, the team members discussed the suggestion. We decided to ask other professors for different project ideas, before choosing a topic.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

16/01/08

Several team members introduced additional project ideas based on former projects and own ideas. Tobias S. agreed on asking Professor Daniel, who supervised the iGEM team 2015, for a different project.

16/01/12

Tobias S. introduced the project proposed by Professor Daniel. The project would aim at constructing a synthetic Vitamin B₁₂ exporter and testing it in different production organisms. We also discussed advantages of this project idea compared to others.

16/01/??

Decision on the project “Vitamin B₁₂ exporter”

16/01/18

We had the first team meeting with our supervisors discussing a rough timeline with important deadlines. Furthermore, we started planning the project funding by sponsors and internal university funding. We agreed on collecting title ideas and searching literature for Vitamin B₁₂ binding proteins, Vitamin B₁₂ dependent reactions and production organisms for the next meeting.

16/01/??

Deciding on title? (Facebook); organization team?

Katzen liegen nicht faul rum. Sie verschönern den Raum.

16/02/01

We decided to design and print a booklet on our project to send it to possible sponsors. Amongst others, information on the iGEM competition, the background to our project, the team and sponsoring suggestions should be included. The topics were divided between team members. The organization team agreed on writing the email text and the text for a letter to address sponsors.

16/02/15

-

16/02/29

-

Katzen liegen nicht faul rum. Sie verschönern den Raum.

16/03/03

Together with the supervisors, we decided on the three production organisms Salmonella typhimurium TA100, Klebsiella planticola and Escherichia blattae to test the constructs for Vitamin B₁₂ exporters. We did not yet choose different B₁₂ binding proteins, which can be used in the exporter. Further literature research is needed for that. However, we already discussed possible experiments to test the B₁₂ productivity in the production organisms in the end. Those include a photometric, a biological and an antibody assay.

The strains for our production organisms have to be ordered from DSMZ. For a biological assay we also have to search for a B₁₂ auxotrophic strain and a B₁₂-free medium.

16/03/11

?

16/03/18

We registered the team for the IDT website to use their offer for iGEM teams to synthesize our constructs.

16/03/29

Decision on constructs?

Katzen liegen nicht faul rum. Sie verschönern den Raum.

16/04/01

We planned the first steps, which needed to be taken in the lab. We have to grow the strains, make the cells electro competent, proof their identity via 16S PCR and check for possible antibiotic resistances.

More research is needed on the TAT secretion system and possible export signals, as well as possible vector systems. It was decided that they should be inducible by arabinose, suggesting the pBAD vector system.

16/04/11: team, supervisors

After discussing the first results from the lab concerning the antibiotic resistances, it was decided to repeat the experiments with chloramphenicol as additional antibiotic and using different concentrations.

For the following weaks, the team was devided into three groups, one testing antibiotic resistace again, one starting to prepare electro competent cells and the third team working on designing the constructs.

We also agreed on having a regular iGEM meeting with the supervisors every two weeks on Monday, 11 am. Furthermore, the organization team will start writing guide lines for lab work and Ines will organize a log book for signing in lab working hours.

Arrival of iGEM package?

Arrival of constructs?

16/04/25

The results for the antibiotic resistance testing were discussed and it was decided to test the competence of electro competent cells with kanamycin (kan) as antibiotic and a kan-vector provided by the lab.

Tobias S. is in contact with Thermo Fisher for the order of our pBAD vector system.

Ines and Larissa will send out letters with our booklet to potential sponsors. Furthermore, we decided that all team members will participate in Marburg at the meeting for iGEM teams from Germany. Tobias S. organizes the registration.

To respresent iGEM, Miriam will contact the “Göttinger Tageblatt”, Ines, Tobias K., Tobias S. and Alexander will participate as a team at the “Göttinger Altstadtlauf” and Tobias S. Tobias K. and Miriam will present iGEM and our project at a “Bier und Brezeln” presentation.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

16/05/09

We decided to join the meeting of the European iGEM teams in Paris and will book bus tickets and the hotel rooms in the following days.

After the meeting, we took pictures with the T-shirts provided by Zymo Research to post it on our facebook profile.

16/05/18

We discussed the final design for out T-Shirts and possible offers for printing them.

We also want to present our project and iGEM at the “GZMB Sommerfest”. In return, we will offer our support in organizing the event.

Concerning lab work, the transformation of our constructs can start as the vector from Thermo Fisher has arrived.

16/05/23

-

16/05/30: TS, TK, AB, NE, ME, LK, MS, supervisors

We discussed the problems in lab work and worked out a schedule for the following weeks. We will test the electro competent cells and if necessary repeat the preparation.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.

Katzen liegen nicht faul rum. Sie verschönern den Raum.