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+ | pNP-Butyrate Assay | ||
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+ | One method to assess the activity of our designed mutants is a using the pNP-Butyrate degradation assay. pNP-B is similar in structure to the PET monomer, therefore an increase in its degradation could suggest an increase in the degradation of PET. | ||
+ | Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls. | ||
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Revision as of 18:01, 8 October 2016
Results
pNP-Butyrate Assay
One method to assess the activity of our designed mutants is a using the pNP-Butyrate degradation assay. pNP-B is similar in structure to the PET monomer, therefore an increase in its degradation could suggest an increase in the degradation of PET. Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls.