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Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls. | Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls. | ||
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+ | <i>P. putida</i> Growth experiments | ||
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+ | In order to test the ability of <i>P. putida</i> to grow in an electrochemical fuel cell we had to assess its ability to product a biofilm on Graphite-felt sheets like those used in the fuel cell. <br> | ||
+ | We incubated the bacteria in a liquid M9 minimal medium with glucose and hanged a sheet of graphite-felt half inside the medium and half outside. | ||
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+ | <img src='https://static.igem.org/mediawiki/2016/4/45/--BGU_ISRAEL--PutidaElectro1.jpg' class="resultsImg"> | ||
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+ | <b>Fig. *:</b> <i>P. putida</i> grown in a M9 liquid medium with a graphite-felt sheet. As seen in the photo the bacteria have form a biofilm on the sheet. | ||
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Revision as of 18:14, 8 October 2016
Results
One method to assess the activity of our designed mutants is a using the pNP-Butyrate degradation assay. pNP-B is similar in structure to the PET monomer, therefore an increase in its degradation could suggest an increase in the degradation of PET. Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls.
In order to test the ability of P. putida to grow in an electrochemical fuel cell we had to assess its ability to product a biofilm on Graphite-felt sheets like those used in the fuel cell.
We incubated the bacteria in a liquid M9 minimal medium with glucose and hanged a sheet of graphite-felt half inside the medium and half outside.