Difference between revisions of "Team:BGU ISRAEL/Results"

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                             Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls.
 
                             Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls.
 
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                        <img class="resultsImg" src="https://static.igem.org/mediawiki/2016/4/45/--BGU_ISRAEL--PutidaElectro1.jpg">
 
 
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                            <i>P. putida</i> Growth experiments
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                            In order to test the ability of <i>P. putida</i> to grow in an electrochemical fuel cell we had to assess its ability to product a biofilm on Graphite-felt sheets like those used in the fuel cell. <br>
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                            We incubated the bacteria in a liquid M9 minimal medium with glucose and hanged a sheet of graphite-felt half inside the medium and half outside.
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                                <b>Fig. *:</b> <i>P. putida</i> grown in a M9 liquid medium with a graphite-felt sheet. As seen in the photo the bacteria have form a biofilm on the sheet.
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Revision as of 18:14, 8 October 2016

PlastiCure

Results

pNP-Butyrate Assay

One method to assess the activity of our designed mutants is a using the pNP-Butyrate degradation assay. pNP-B is similar in structure to the PET monomer, therefore an increase in its degradation could suggest an increase in the degradation of PET. Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls.

P. putida Growth experiments

In order to test the ability of P. putida to grow in an electrochemical fuel cell we had to assess its ability to product a biofilm on Graphite-felt sheets like those used in the fuel cell.
We incubated the bacteria in a liquid M9 minimal medium with glucose and hanged a sheet of graphite-felt half inside the medium and half outside.

Fig. *: P. putida grown in a M9 liquid medium with a graphite-felt sheet. As seen in the photo the bacteria have form a biofilm on the sheet.

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Ben-Gurion University of the Negev
Ben Gurion 1, Beer Sheva 8410501, Israel

Mail: igembgu2016@gmail.com

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