Difference between revisions of "Team:UPO-Sevilla/Notebook"

Revision as of 08:58, 11 October 2016

JANUARY

Monday	Tuesday	Wednesday	Thursday	Friday
				1
4	5	6	7	8
11	12	13	14	15
18	19	20	21	22
25	26	27	28	29

FEBRUARY

Monday	Tuesday	Wednesday	Thursday	Friday
1	2	3	4	5
8	9	10	11	12
15	16	17	18	19
22	23	24	25	26
29

MARCH

Monday	Tuesday	Wednesday	Thursday	Friday
	1	2	3	4
7	8	9	10	11
14	15	16	17	18
21	22	23	24	25
28	29	30	31

APRIL

Monday	Tuesday	Wednesday	Thursday	Friday
				1
4	5	6	7	8
11	12	13	14	15
18	19	20	21	22
25	26	27	28	29

MAY

Monday	Tuesday	Wednesday	Thursday	Friday
2	3	4	5	6
9	10	11	12	13
16	17	18	19	20
23	24	25	26	27
30	31

JUNE

Monday	Tuesday	Wednesday	Thursday	Friday
		1	2	3
6	7	8	9	10
13	14	15	16	17
20	21	22	23	24
27	28	29	30

JULY

Monday	Tuesday	Wednesday	Thursday	Friday
				1
4	5	6	7	8
11	12	13	14	15
18	19	20	21	22
25	26	27	28	29

AUGUST

Monday	Tuesday	Wednesday	Thursday	Friday
1	2	3	4	5
8	9	10	11	12
15	16	17	18	19
22	23	24	25	26
29	30	31

SEPTEMBER

Monday	Tuesday	Wednesday	Thursday	Friday
			1	2
5	6	7	8	9
12	13	14	15	16
19	20	21	22	23
26	27	28	29	30

JANUARY

6^th January

Prepare plates with LB+Km and plate the strain with pSB1K3 (E. coli DH5α)

Back to the calendar

7^th January

Inocula of pSB1K3 in 15 mL of LB+Km (kanamycin)

Back to the calendar

8^th January

Minipreps of pSB1K3

Plate the strains of E. coli DH5α with pMPO364 (nasF and nahR-Psal), JB3Tc19::PleD (pleD*), pMPO52 (Pm) and pMRB11(yhjH), and P. putida KT2442 (lapG)

Back to the calendar

11^th January

Put one colony of each strain in 3 mL of LB+specific antibiotic and incubate overnight

Back to the calendar

12^th January

Minipreps and measure the concentration of the cultures

Back to the calendar

13^th January

14 first primers have arrived. Resuspend them, dilute until 10 μM and storage at -20ºC

PCR of pMPO364 and pMRB11 [45ºC]

PCR of pJBeTc19::pleD and Pseudomonas putida KT2442 chromosome [60ºC]

Electrophoresis of both PCR

Back to the calendar

14^th January

Repeat the PCR at 45ºC and at 60ºC

Electrophoresis of both PCR

Purify the 5 PCR products (lapG, nahR-Psal, nasF, pleD*, yhjH) and store them at -20ºC

Back to the calendar

15^th January

Digest the purified PCR products and pSB1K3 where we will clone the PCR products

Electrophoresis of the digested PCR products and vector and cut a slice of the gel that contain the fragment of interest and purify

Plate the strain with pMPO1035 (xylS2)

Back to the calendar

18^th January

Ligation the vector with each PCR product

Put one colony of pMPO1035 in 3 mL of LB+Cm (Chloramphenicol)

Back to the calendar

19^th January

Transformation of competent cells, E. coli DH5α, with the ligations

Miniprep and measure the concentration of pMPO1035

Back to the calendar

20^th January

Inocula of each transformation event in 3 mL of LB+Km

PCR of pMPO1035 Back to the calendar

21^th January

Miniprep the plasmid DNA of the transformations

Diagnostic digest the plasmids with PstI and XbaI

Electrophoresis of the digestions and the PCR. All the fragments had the correct length

Purify the PCR of xylS2 and digest with PstI and XbaI

Purify the digestion of xylS2 and ligation with pSB1K3

Back to the calendar

22^th January

Transformation of competent cells, E. coli DH5α, with the ligation

Back to the calendar

25^th January

Repeat the ligation of xylS2 with the vector, because the plates were contaminated

Back to the calendar

26^th January

Send to sequence 4 constructions (lapG, nahR-Psal, nasF, yhjH)

Transformation of competent cells, E. coli DH5α, with the ligation

Back to the calendar

27^th January

The transformation was unsuccessful, so we did a electrophoresis of the vector and the insert (xylS2) to test that we have DNA in the sample

Repeat the ligation of xylS2 with the vector

Dilute until 10 μM the primers that we use to do a overlap extension PCR

Back to the calendar

28^th January

Test the received sequences of lapG, nahR-Psal, nasF and yhjH. All of them were correct

Transformation of competent cells, E. coli DH5α, with the ligation and the tested constructions. Now we name these constructions as pMRB120 (nahR-Psal), pMRB121 (lapG), pMRB122 (yhjH) and pMRB123 (nasF)

Digest pMRB121, pMRB122 and pMRB123 as vector and pMRB120 as insert

Back to the calendar

29^th January

Electrophoresis of the digestions and cut a slice that contains the fragment of interest and purify

Back to the calendar

FEBRUARY

1^st February

Inocula of each transformation and xylS2 in 3 mL of LB+Km

Ligation of yhjH, lapG and nasF with nahR-Psal

Back to the calendar

2^nd February

Miniprep and diagnostic digestion of xylS2

Storage the pMRB120, pMRB121, pMRB122 and pMRB123 strains at -80ºC

Transformation of competent cells, E. coli DH5α, with the ligations

Send to sequence the constructions of xylS2 and pleD*

Back to the calendar

3^th February

Take biomass of the transformations and plate onto other plate, because there were too many cells on the plates

First part of overlap extension PCR of Pm

Back to the calendar

4^th February

Inocula of the transformation in 3 mL of LB+Km

Electrophoresis of PCR of Pm, but it did not go well

Repeat the PCR of Pm

Back to the calendar

5^th February

Miniprep of nahR-Psal-lapG, nahR-Psal-yhjH and nahR-Psal-nasF

Diagnostic digest of these plasmids

Test the received sequences of xylS2 and pleD*. Only xylS2 is correct, so do a transformation to introduce the plasmid, now we name this construction as pMRB124

Back to the calendar

8^th February

Inocula of xylS2 strain in LB+Km

Transformation with nahR-Psal-yhjH V, nahR-Psal-lapG IV and nahR-Psal-nasF II. Now, we name the constructions as pMRB125, pMRB126 and pMRB127, respectively

Electrophoresis of PCR product, but only Pm RM 1 (the fragment of Pm amplified with the primer reverse and mutagenic forward 1) did not go well

Back to the calendar

9^th February

Inocula of each transformation in LB+Km

Storage the pMRB124 strain at -80ºC

Repeat the PCR of Pm RM 1

Electrophoresis of the PCR

Back to the calendar

10^th February

Storage the pMRB125, pRMB126 and pMRB127 strains at -80ºC

Purify all the PCR products of Pm

Digest pMRB127 with EcoRI and SpeI as insert

Ligation of the vector with pleD*, and lapG and yhjH with nahR-Psal-nasF

Back to the calendar

11^th February

Electrophoresis of the digestion of pMRB127, cut the slice that contains the fragment of interest and purify

Transformation with the ligations

Overlap extension PCR of Pm1, Pm2 and Pm3, and PCR of Pm RM1 and Pm (In)

Electrophoresis of the overlap extension PCR

Back to the calendar

12^th February

Electrophoresis of Pm RM1 and Pm FM1 to compare the concentrations. Dilute 10 times and repeat the overlap extension PCR

Electrophoresis of the overlap extension PCR of Pm1. It does not go well

Back to the calendar

15^th February

Inocula of nahR-Psal-nasF-lapG and nahR-Psal-nasF-yhjH (complex devices), pUC18Sfi;miniTn7BBGm and pleD* in LB

Repeat overlap extension PCR of Pm1

Back to the calendar

16^th February

Miniprepion of the plasmid DNA of the cultures

Diagnostic digestion of pleD* and complex devices

Electrophoresis of the diagnostic digestions and the PCR of Pm1

Back to the calendar

17^th February

Transformation with pleD* and complex devices. Now, we name the constructions as pMRB145, pMRB129 and pMRB130, respectively

Digest Tn7 and the vector as vector and Pm, Pm1, Pm2, Pm3, nahR-Psal-nasF-lapG and nahR-Psal-nasF-yhjH

Ligation of the vector with Pm, Pm1, Pm2 and Pm3 (Pm vectors), and Tn7 with nahR-Psal-nasF-lapG and nahR-Psal-nasF-yhjH (Tn7 devices)

Back to the calendar

18^th February

Inocula of each transformation in LB+Km

Transformation with the ligations

Back to the calendar

19^th February

Miniprep of pleD* and complex devices

Storage pMRB145, pMRB129 and pMRB130 at -80ºC

Back to the calendar

22^th February

Incocula of each transformation in LB

Plate the strains with pMRB124 (xylS2) and pTNS2 (helper of Tn7)

Digest pMRB129 and pMRB130 as insert

Back to the calendar

23^th February

Miniprep of the cultures

Electrophoresis of the digestions, cut a slice that contains the fragment of interest and purify

Inocula of 124 and pTNS2 in 3 mL of LB

Back to the calendar

24^th February

Diagnostic digestion of Pm vectors and Tn7 devices

Electrophoresis of the diagnostic digestions of Pm vectors and Tn7 devices

Miniprep of the cultures of 124 and pTNS2

Send to sequence the constructions of Pm

Back to the calendar

25^th February

Repeat the transformation with Tn7 devices. Now, we name the constructions as pMRB133 (Tn7-nahR-Psal-nasF-lapG) and pMRB134 (Tn7-nahR-Psal-nasF-yhjH)

Digest 124, 120 and pSB1K3 as vector

Ligation of 124 (v) with 120 (i) and 127 (i)

Inocula of pMRB125 and pMRB126 in 3 mL of LB

Back to the calendar

26^th February

Segregate the plates of 133 and 134

Miniprep of the cultures of 125 and 126

Heat-shock transformation with the ligations

Digest 125 and 126 as insert

Back to the calendar

29^th February

Inocula of 133 and 134 in 5 mL of LB and 124+120 in 3 mL of LB

Segregate the plate of 124+127

Heat-shock transformation with the constructions of Pm. Now, we name the constructions as pMRB137 (Pm), pMRB138 (Pm1), pMRB139 (Pm2) and pMRB140 (Pm3)

Electrophoresis of the digestions of 125 and 126, cut a slice of gel that contains the fragment of interest and purify

Back to the calendar

MARCH

1^st March

Miniprep of the cultures

Storage pMRB133 and pMRB134 at -80ºC

Inocula of 124+127 in 3 mL of LB, and each construction of Pm in 5 mL of LB

Back to the calendar

2^nd March

Miniprep of the culture

Segregate the plates of the transformations

Digest and electrophoresis of 124+127

Ligation Tn7 with 125 (i) and 126 (i)

Back to the calendar

3^th March

Storage pMRB137, pMRB138, pMRB139 and pMRB140 at -80ºC

Transformation of the ligations and pleD*. Now, we name the construction of pleD* as pMRB145

Digest 124 as vector

Electrophoresis of the digestion, cut a slice of gel that contains the fragment of interest and purify

Ligation 124 (v) with 120 (i) and 127 (i), and Tn7 with 125 (i) and 126 (i)

Back to the calendar

4^th March

Segregate the plate of the transformation

Heat-shock transformation with the ligations

Plate the strain with pMRB1 (vector that contains the protein fusion gen lapZ-gfpmut3)

Back to the calendar

7^th March

Inocula of 145 in 5 mL of LB

Inocula of pMRB1,124+120 and 124+127 in 3 mL of LB Back to the calendar

8^th March

Storage pMRB145 at -80ºC

Miniprep of the cultures

Digest 145 as vector and 137, 138, 139 and 140 as insert

Electrophoresis of the digestions, cut a slice of gel that contains the fragment of interest and purify

Diagnostic digestion of the pMRB124 constructions

Back to the calendar

9^th March

Electrophoresis of the diagnostic digestions

Digest pMRB1 as vector

Repeat the ligation of Tn7 with 125 (i) and 126 (i), and 145 (v) with 120 (i) and 127 (i)

Segregate the plate of 124+127. Now, we name this construction as pMRB146

Back to the calendar

10^th March

Transformation with the ligations

Electrophoresis of the digestion of pMRB1 (v), cut a slice of gel that contains the fragment of interest and purify

Ligation of pMRB1 (v) with the four Pm (i)

Back to the calendar

11^th March

Transformation with the ligations

Plate the strain with 128

Digest of pMRB125 and pMRB126 as insert

Back to the calendar

14^th March

Inocula of 145+120, 145+127, Pm contructions, 128 and 146

Diagnostic digestion of 145+120 and 145+127

Ligation of Tn7 with 120 (i) and 127 (i), and 124 (v) with 120 (i)

Back to the calendar

15^th March

Miniprep of the cultures and 128 digest as vector

Diagnostic digestion of the Pm constructions

Storage pMRB146 at -80ºC

Transformation with the ligations

Back to the calendar

16^th March

Electrophoresis of the diagnostic digestions

Electrophoresis of the digestions of 125 (i) and 126 (i), cut a slice of gel that contains the fragment of interest and purify

Ligation of Tn7 with 120, 125 and 126, and 145 (v) with 120 (i)

Inocula of Tn7+127 and 124+120

Back to the calendar

17^th March

Miniprep of the cultures

Diagnostic digestion of 124+120 and Tn7+127

Transformation with the ligations

Back to the calendar

18^th March

The transformations with Tn7 devices didn't work, so plate Tn7 strain again

Transformation with 145+127. Now, we name this construction as pMRB147

Back to the calendar

21^th March

Inocula of Tn7, 147, 145+120 and 145+127

Repeat the ligation of pMRB1 (v) with 137 (i)

Back to the calendar

22^th March

Miniprep of the cultures

Diagnostic digestion of 145+127 and 145+120

Electrophoresis of pMRB128 (v), cut a slice of gel that contains the fragment of interest and purify

Transformation with pMRB1+137 and Tn7+127

Ligation of Tn7 (v) with 125 (i)

Back to the calendar

23^th March

Inocula of pMRB1+137

Transformation with Tn7+pMRB125

Electrophoresis of the diagnostic digestion of 145+120

Back to the calendar

24^th March

Minipreps and diagnostic digestion of 1+137

Segregate the plate of Tn7+127

Digestion of 145 and 147 as an insert

Ligation of Tn7 (v) with 125 (i) and 126 (i), and 145 (v) with 120 (i)

Back to the calendar

25^th March

Transformation of the ligations

Electrophoresis of the digestions, cut a slice of gel that contains the fragment of interest and purify

Diagnostic digestion of pMRB1+137/138/139/140

Back to the calendar

28^th March

Inocula of Tn7+127. Now, we name this construction as pMRB151

The transformations did not go well

Back to the calendar

29^th March

Ligation of 120 (v) with 124 (i), Tn7 (v) with 125 (i), 147 (i) and 126 (i), pMRB1 (v) with 137-140 (i) and 145 (v) with 120 (i)

Store at -80ºC and minipreps of pMRB151

Back to the calendar

30^th March

Transformation of the ligations

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31^th March

Inocula of 1+137/138/139/140, 120+145,120+124 and Tn7+147

Segregate the transformation of Tn7+126

Back to the calendar

APRIL

1^st April

Minipreps and diagnostic digestion of the 1+137/138/139/140, 120+124, 145+120 and Tn7+147

Electrophoresis of the diagnostic digestion

Back to the calendar

4^nd April

2nd diagnostic digestions of 1+137/138/139/140

Inocula of 124,135 and 136

Transformation of 145+120 and Tn7+147. Now, we name these constructions as pMRB135 and pMRB136, respectively

Back to the calendar

5^th April

Storage pMRB135 and pMRB136 at -80ºC

Inocula of KT2442

Digest 135,145 and 130 as insert and Tn7 as vector

Measure the concentration of pTNS2, pMRB136 and pMRB134>

Back to the calendar

6^th April

Electrophoresis of digestion of 135, 145, 130 and Tn7, cut a slice of gel that contains the fragment of interest and purify

Plate the strains with pMRB136 and pTNS2

Electroporation of KT2442 with 136 and 134

Back to the calendar

7^th April

Inocula of pTNS2 and 136

Ligation of Tn7 with 126 and 135

Colony PCR of the electroporations

Back to the calendar

8^th April

Minipreps of pTNS2 and 136

Transformation of the ligations

Electrophoresis of the colony PCR

Back to the calendar

11^th April

Inocula of Tn7+126 and Tn7+135

Ligation of 1 (v) with 138 (i), 124 (v) with 120 (i) and Tn7 (v) with 120 (i) and 125 (i)

Back to the calendar

12^th April

Minipreps and diagnostic digestion of the cultures

Transformation of the ligations

Back to the calendar

13^th April

Inocula of 1+138, 124+120, and Tn7+125. Now ,we name 124+120 as pMRB152

Ligation of Tn7 (v) with 130 (i), 145 (i), 146 (i) and 120 (i)

Back to the calendar

14^th April

Minipreps of the cultures

Storage pMRB152 at -80ºC

Diagnostic digestion and electrophoresis of 1+137/138/139/140, Tn7+125, Tn7+135 and Tn7+126

Transformation with Tn7+120, Tn7+145, Tn7+130, Tn7+146 and Tn7+125

Back to the calendar

15^th April

Measure the concentration of 136 and 134

Diagnostic digestions of 1+138, Tn7+126 and Tn7+135

Plate the strain with pTNS2

Back to the calendar

18^th April

Inocula of KT2442, pTNS2, Tn7+145, Tn7+130, Tn7+146, Tn7+120 and Tn7+125. Now, we name Tn7+125 as pMRB128, Tn7+130 as pMRB164 and Tn7+145 as pMRB165

Electrophoresis of the diagnostic digestions

Back to the calendar

19^th April

Electroporation of KT2442 with 164, 165, 134 and 136

Minipreps of pTNS2, Tn7+146 and Tn7+120

Storage pMRB128, pMRB164 and pMRB165 at -80ºC

Transformation of 1+Pms. Now, we name 1+Pm, 1+Pm1, 1+Pm2 and 1+Pm3 as pMRB166, pMRB167, pMRB168 and pMRB169

Back to the calendar

20^th April

Colony PCR of the electroporations and electrophoresis

Diagnostic digestion of Tn7+146 and Tn7+120

Segregate the positive candidates of electroporations

Plate KT2442-Tn7

Inocula of 166, 167, 168 and 169

Back to the calendar

21^th April

Inocula of KT2442-Tn7, KT2442-T134, KT2442-T136, KT2442-T164 and KT2442-T165

Electrophoresis of Tn7+146 and Tn7+120

Diagnostic digestion of 120 and 146

Storage at -80ºC and minipreps of pMRB166-169

Back to the calendar

22^th April

Digest 152 as insert

Electroporation of KT2442-T134, KT2442-T136, KT2442-T164 and KT2442-T165 with pCdrA

Back to the calendar

25^th April

Plate the strains KT2442-Tn7, KT2442-T134, KT2442-T136, KT2442-T164 and KT2442-T165

Inocula for fluorimetry

Electrophoresis of 152 (i), cut a slice of gel that contains the fragment of interest and purify

Back to the calendar

26^th April

Digest pSB1K3 as vector

Inocula of KT2442-Tn7, KT2442-T134, KT2442-T136, KT2442-T164 and KT2442-T165

Fluorimeter

Back to the calendar

27^th April

INSTANT curves of growth and biofilm of KT2442-Tn7/T134/T136/T164/T165

Failed fluorimeter

Back to the calendar

28^th April

Dye and measure the plates of INSTANT curves

Digest 120 as insert

Ligation Tn7+146 and Tn7+152

Back to the calendar

29^th April

Transformation of the ligations

Plate 128, 133, KT2442-Tn7/T136/T165 and KT2442, KT2442 lapG^- and KT2442 ΔbifA

Back to the calendar

MAY

2^st May

Inocula of Tn7+146, Tn7+152, KT2442 lapG^-, 128 and 133

Plate all the KT2442 with pCdrA, KT2442 ΔfleQ and all the variants of KT2442 of pleD*

Electrophoresis of 120 (i), cut a slice of gel that contains the fragment of interest and purify

Back to the calendar

3^nd May

Minipreps and measure the concentration of 128 and 133

Minipreps and diagnostic digestion of Tn7+146 and Tn7+152

Electroporation of KT2442 lapG^- with 128 and 133

Back to the calendar

4^th May

Inocula for curves and fluorimeter

Electrophoresis of the diagnostic digestions

Dilutions of fluorimeter and curves of KTs

Back to the calendar

5^th May

Dye and measure the curves of pleD*

Take the data of the fluorimeter

Back to the calendar

6^th May

Plate the variants of KT2442 with yhjH

Transformation of Tn7+120

Back to the calendar

9^th May

Inocula of KT2442-Tn7/T134/T164 and Tn7+120

Transformation with Tn7+146 and Tn7+152. Now, we name these strains as pMRB159 and pMRB170, respectively

Back to the calendar

10^th May

Minipreps and diagnostic digestion of Tn7+120

Dilutions of yhjH and put the curves for 20 h

Segregate KT2442 lapG^--T128/T133

Back to the calendar

11^th May

Dye and measure the plates of yhjH

Inocula of 159 and 170

Electrophoresis of the diagnostic digestion

Colony PCR of KT2442 lapG^--T128/T133

Transformation with Tn7+120

Back to the calendar

12^th May

Minipreps of the cultures

Storage pMRB159 and pMRB170 at -80ºC

Inocula of Tn7+120

Back to the calendar

13^th May

PCR of pMRB1 to get the gene gfp

Electrophoresis of the colony PCRs

Segregate Tn7+120

Back to the calendar

16^th May

Inocula of pleD* constructions in Tn7 and KT2442 lapG^--T128

Inocula of Tn7+120

Colony PCR and electrophoresis of KT2442 lapG^--T133

Back to the calendar

17^th May

Storage KT2442 lapG^--T128 at -80ºC

Dilutions of pleD* curves

Plate KT2442 lapG^- and KT2442 ΔbifA

Inocula of KT2442

Inocula of KT2442 and KT2442-Tn7/T134/T136/T164/T165 for Congo Red and curves

Back to the calendar

18^th May

Dye and measure the pleD* plates

Plate the strains with 134, 136, 164, 170, 159 and Tn7

Inocula of KT2442-Tn7/T165 for curves

Inocula of KT2442 lapG^- and KT2442 ΔbifA

Plates of Congo Red

Curves of 134, 164 and KT2442

Back to the calendar

19^th May

Electrophoresis of the PCR of gfp

Curves of 165

Electroporation of KT2442 lapG^- and ΔbifA with Tn7, 128 and 133

Dye and measure the plates of 134, 164 and KT2442

Let more time the plates with Congo Red

Back to the calendar

20^th May

Dye and measure the plates of 165 at 17 h and 20 h

Take a photo of the plates with Congo Red

Let the plates with Congo Red at RT overnight

Back to the calendar

23^th May

Inocula of 170, 159 and Tn7

Electroporation of KT2442 with 159, 170, 128 and 133

Minipreps and measure the concentration of 170, 159 and Tn7

Digestion of 164 and 148

PCR of gfp

Electrophoresis of the digestions and PCR

Back to the calendar

24^th May

Colony PCR adn electrophoresis of the electroporations

Minipreps and measure the concentration of 170, 159 and Tn7

Plate all the strains with pCdrA and the same strains withour pCdrA (fluorimeter and Congo Red)

Inocula of KT2442-T170/T159/T128, lapG^--Tn7 and ΔbifA-T128

Back to the calendar

25^th May

Inocula of the plates of yesterday

Colony PCR and electrophoresis of KT2442 lapG^--Tn7/T133, ΔbifA-T128/T133 and KT2442-T133

Storage KT2442-T170/T159/T128, lapG^--Tn7 and ΔbifA-T128 at -80ºC

Back to the calendar

26^th May

Assay of the fluorimeter with all the constructions and ΔfleQ

Plate of Congo Red with KT2442, Tn7/T134/T136/T164/T165

Colony PCR and electrophoresis of KT2442-T133, KT2442 lapG^--T133 and KT2442 ΔbifA-T133

Back to the calendar

27^th May

Get the results of the fluorimeter

Let the plates with Congo Red 24 h more

Electrophoresis of the digestion of 164, cut a slice of gel that contains the fragment of interest and purify

Back to the calendar

30^th May

Plate KT2442, KT2442-T134/T164, KT2442-Tn7/T128 and lapG^--Tn7/T128

A labmate got the plates with Congo Red and took a photo of >

Back to the calendar

31^th May

Inocula of KT2442-T134/T164 and KT2442-Tn7/T128 and lapG^--Tn7/T128

Plate pMRB133

Back to the calendar

JUNE

1^st June

Dilutions and prepare curves of KT2442-T134/T164 and KT2442-Tn7/T128 and lapG^--Tn7/T128

Inocula of KT2442-Tn7/T128 and lapG^--Tn7/T128

Back to the calendar

2^nd June

Dye and measure the plates of KT2442-T134/T164 and KT2442-Tn7/T128 and lapG^--Tn7/T128

Dilutions and prepare curves of KT2442-Tn7/T128 and lapG^--Tn7/T128

Inocula of 133

Back to the calendar

3^th June

Dye and measure the plates of KT2442-Tn7/T128 and lapG^--Tn7/T128

Miniprep and measure the concentration of 133

Back to the calendar

6^th June

Plate KT2442-Tn7/T134/T164 and KT2442-Tn7/T128 and lapG^--Tn7/T128

Diagnostic digestion and electrophoresis of 133

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7^th June

Inocula of KT2442-Tn7/T134/T164 and KT2442-Tn7/T128 and lapG^--Tn7/T128

Electroporation of KT2442, KT2442 lapG^- and ΔbifA with pMRB133

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8^th June

Dilutions and prepare curves of KT2442-T134/T164 and KT2442-Tn7/T128 and lapG^--Tn7/T128

Colony PCR and electrophoresis of 133 electroporations

Inocula of KT2442/lapG^-/ΔbifA-T133

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9^th June

Dye and measure the plates of KT2442-T134/T164 and KT2442-Tn7/T128 and lapG^--Tn7/T128

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10^th June

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13^th June

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14^th June

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15^th June

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16^th June

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17^th June

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20^th June

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21^th June

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22^th June

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24^th June

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27^th June

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28^th June

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29^th June

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30^th June

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JULY

1^st July

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4^nd July

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5^th July

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6^th July

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7^th July

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8^th July

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11^th July

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12^th July

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13^th July

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14^th July

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15^th July

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18^th July

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19^th July

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20^th July

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21^th July

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22^th July

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25^th July

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26^th July

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27^th July

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28^th July

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29^th July

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AUGUST

1^st August

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2^nd August

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3^th August

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4^th August

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5^th August

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8^th August

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9^th August

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10^th August

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11^th August

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12^th August

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15^th August

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16^th August

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17^th August

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18^th August

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19^th August

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22^th August

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23^th August

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24^th August

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25^th August

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26^th August

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29^th August

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29^th August

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29^th August

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SEPTEMBER

1^st September

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2^nd September

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5^th September

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6^th September

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7^th September

Back to the calendar

8^th September

Back to the calendar

9^th September

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12^th September

Back to the calendar

13^th September

Back to the calendar

14^th September

Back to the calendar

15^th September

Back to the calendar

16^th September

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19^th September

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20^th September

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21^th September

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22^th September

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23^th September

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26^th September

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27^th September

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28^th September

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29^th September

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30^th September

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@@ Line 1: / Line 1: @@
-{{UPO-Sevilla}}
+{{:Team:UPO-Sevilla/Template:CSS}}
-<html>
+{{:Team:UPO-Sevilla/Template:JAVA}}
+<html>
+<style type="text/css">
+			* {
+				margin:0px;
+				padding:0px;
+			}
+			#header {
+				margin:auto;
+				width:500px;
+				font-family:Arial, Helvetica, sans-serif;
+			}
+			ul, ol {
+				list-style:none;
+			}
+			.nav > li {
+				float:left;
+			}
+			.nav li a {
+				background-color:#CEECF5;
+				color:#000;
+				text-decoration:none;
+				padding:10px 12px;
+				display:block;
+			}
+			.nav li a:hover {
+				background-color:#434343;
+			}
+			.nav li ul {
+				display:none;
+				position:absolute;
+				min-width:140px;
+			}
+			.nav li:hover > ul {
+				display:block;
+			}
+			.nav li ul li {
+				position:relative;
+			}
+			.nav li ul li ul {
+				right:-140px;
+				top:0px;
+			}
+		</style>
+  <!--HEADER ROW-->
+  <div id="header-row">
+    <div class="container" style="height:75px">
+      <div class="row" style="height:75px">
+              <!--LOGO-->
+              <div class="span3"><a class="brand" href="#"><img src="https://static.igem.org/mediawiki/2016/9/94/T--UPO-Sevilla--upoescudo.jpg" width="200" height="400"></a></div>
+              <!-- /LOGO -->
-<div class="column full_size">
+            <!-- MAIN NAVIGATION -->
+              <div class="span9">
+                <div class="navbar  pull-right" style="font-size:80%">
+                  <div class="navbar-inner">
+                    <a data-target=".navbar-responsive-collapse" data-toggle="collapse" class="btn btn-navbar"><span class="icon-bar"></span><span class="icon-bar"></span><span class="icon-bar"></span></a>
+                    <div class="nav-collapse collapse navbar-responsive-collapse">
+                    <ul class="nav">
+                        <li><a href="https://2016.igem.org/Team:UPO-Sevilla">Home</a></li>
+						<li class="dropdown">
+                          <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
+                            <ul class="dropdown-menu">
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Team">Members</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Attributions">Attributions</a></li>
+                            </ul>
+                        </li>
+						<li class="dropdown">
+                          <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
+                            <ul class="dropdown-menu">
+                                  <li><a href="about.html">Overview</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Description">Description</a></li>
+                            </ul>
+                        </li>
+                        <li class="dropdown">
+                          <a href="about.html" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a>
+                            <ul class="dropdown-menu">
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Basic_Part">Basic Parts</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Composite_Part">Composite Parts</a></li>
+								  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Part_Collection">Part Collection</a></li>
+                            </ul>
+                        </li>
-<p> Document the dates you worked on your project.</p>
+						<li class="dropdown">
+                          <a href="about.html" class="dropdown-toggle" data-toggle="dropdown">Wet Lab<b class="caret"></b></a>
+                            <ul class="dropdown-menu">
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Protocols">Protocols</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Notebook">Notebook</a></li>
+								  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Safety">Safety</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Results">Results</a></li>
+                            </ul>
+                        </li>
+                        <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Collaborations">Collaborations</a></li>
+                        <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Human_Practices">Human Practices</a></li>
+						<li><a href="https://2016.igem.org/Team:UPO-Sevilla/Model">Modeling</a></li>
+                    </ul>
+                  </div>
-</div>
+                  </div>
+                </div>
+              </div>
+            <!-- MAIN NAVIGATION -->
+      </div>
+    </div>
+  </div>
+<!-- /HEADER ROW -->
-<div class="column half_size">
+<body>
-<h5>What should this page have?</h5>
-<ul>
-<li>Chronological notes of what your team is doing.</li>
-<li> Brief descriptions of daily important events.</li>
-<li>Pictures of your progress. </li>
-<li>Mention who participated in what task.</li>
-</ul>
-</div>
-<div class="column half_size">
+  <!--HEADER ROW-->
-<h5>Inspiration</h5>
+  <div id="header-row">
-<p>You can see what others teams have done to organize their notes:</p>
+    <div class="container">
+      <div class="row">
+              <!--LOGO-->
+              <div class="span3"><a class="brand" href="#"><img src="img/upoescudo.jpg" width="200" height="400"></a></div>
+              <!-- /LOGO -->
-<ul>
+            <!-- MAIN NAVIGATION -->
-<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
+              <div class="span9">
-<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
+                <div class="navbar  pull-right">
-<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
+                  <div class="navbar-inner">
-<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
+                    <a data-target=".navbar-responsive-collapse" data-toggle="collapse" class="btn btn-navbar"><span class="icon-bar"></span><span class="icon-bar"></span><span class="icon-bar"></span></a>
-</ul>
+                    <div class="nav-collapse collapse navbar-responsive-collapse">
+                    <ul class="nav">
+                        <li><a href="https://2016.igem.org/Team:UPO-Sevilla">Home</a></li>
+						<li class="dropdown">
+                          <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
+                            <ul class="dropdown-menu">
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Team">Members</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Attributions">Attributions</a></li>
+                            </ul>
+                        </li>
+						<li class="dropdown">
+                          <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
+                            <ul class="dropdown-menu">
+                                  <li><a href="about.html">Overview</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Description">Description</a></li>
+                            </ul>
+                        </li>
+                        <li class="dropdown">
+                          <a href="about.html" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a>
+                            <ul class="dropdown-menu">
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Basic_Part">Basic Parts</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Composite_Part">Composite Parts</a></li>
+								  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Part_Collection">Part Collection</a></li>
+                            </ul>
+                        </li>
-</div>
+						<li class="dropdown">
+                          <a href="about.html" class="dropdown-toggle" data-toggle="dropdown">Wet Lab<b class="caret"></b></a>
+                            <ul class="dropdown-menu">
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Experiments">Protocols</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Notebook">Notebook</a></li>
+								  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Safety">Safety</a></li>
+                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Results">Results</a></li>
+                            </ul>
+                        </li>
+                        <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Collaborations">Collaborations</a></li>
+                        <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Human_Practices">Human Practices</a></li>
+						<li><a href="https://2016.igem.org/Team:UPO-Sevilla/Model">Modeling</a></li>
+                    </ul>
+                  </div>
+                  </div>
+                </div>
+              </div>
+            <!-- MAIN NAVIGATION -->
+      </div>
+    </div>
+  </div>
+  <!-- /HEADER ROW -->
+ <!--PAGE TITLE-->
+	<div class="row">
+		<div class="page-header" style="text-align:center">
+				<h1><a name="calendar">
+				NOTEBOOK
+				</a></h1>
+		</div>
+		</div>
+  <!-- /. PAGE TITLE-->
+ <body>
+    <div id="cuerpo" style="height:200px">
+		<div id="izquierda" style="text-align:center;margin-left:55px;margin-right:53px">
+		<p><b>JANUARY</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td></td>
+                        <td></td>
+						<td></td>
+						<td></td>
+						<td><a href="#11">1</a></td>
+                     </tr>
+                     <tr>
+                        <td><a href="#14">4</a></td>
+                        <td><a href="#15">5</a></td>
+						<td><a href="#16">6</a></td>
+						<td><a href="#17">7</a></td>
+						<td><a href="#18">8</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#111">11</a></td>
+                        <td><a href="#112">12</a></td>
+						<td><a href="#113">13</a></td>
+						<td><a href="#114">14</a></td>
+						<td><a href="#115">15</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#118">18</a></td>
+                        <td><a href="#119">19</a></td>
+						<td><a href="#120">20</a></td>
+						<td><a href="#121">21</a></td>
+						<td><a href="#122">22</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#125">25</a></td>
+                        <td><a href="#126">26</a></td>
+						<td><a href="#127">27</a></td>
+						<td><a href="#128">28</a></td>
+						<td><a href="#129">29</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+		<div id="centro" style="text-align:center;margin-left:82px">
+		<p><b>FEBRUARY</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td><a href="#21">1</a></td>
+                        <td><a href="#22">2</a></td>
+						<td bgcolor="#FE2E2E"><a href="#23">3</a></td>
+						<td><a href="#24">4</a></td>
+						<td><a href="#25">5</a></td>
+						<!--<td><a href=""></a></td>-->
+                     </tr>
+                     <tr>
+                        <td><a href="#28">8</a></td>
+                        <td><a href="#29">9</a></td>
+						<td><a href="#210">10</a></td>
+						<td><a href="#211">11</a></td>
+						<td><a href="#212">12</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#215">15</a></td>
+                        <td><a href="#216">16</a></td>
+						<td><a href="#217">17</a></td>
+						<td><a href="#218">18</a></td>
+						<td><a href="#219">19</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#222">22</a></td>
+                        <td><a href="#223">23</a></td>
+						<td><a href="#224">24</a></td>
+						<td><a href="#225">25</a></td>
+						<td><a href="#226">26</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#229">29</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+		<div id="derecha" style="text-align:center;margin-left:135px;margin-right:55px">
+		<p><b>MARCH</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td></td>
+                        <td><a href="#31">1</a></td>
+						<td><a href="#32">2</a></td>
+						<td><a href="#33">3</a></td>
+						<td><a href="#34">4</a></td>
+						<!--<td><a href=""></a></td>-->
+                     </tr>
+                     <tr>
+                        <td><a href="#37">7</a></td>
+                        <td><a href="#38">8</a></td>
+						<td><a href="#39">9</a></td>
+						<td><a href="#310">10</a></td>
+						<td><a href="#311">11</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#314">14</a></td>
+                        <td><a href="#315">15</a></td>
+						<td><a href="#316">16</a></td>
+						<td><a href="#317">17</a></td>
+						<td><a href="#318">18</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#321">21</a></td>
+                        <td><a href="#322">22</a></td>
+						<td><a href="#323">23</a></td>
+						<td><a href="#324">24</a></td>
+						<td><a href="#325">25</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#328">28</a></td>
+                        <td><a href="#329">29</a></td>
+						<td><a href="#330">30</a></td>
+						<td><a href="#331">31</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+	</div>
+	<div id="cuerpo" style="height:200px">
+		<div id="izquierda" style="text-align:center;margin-left:55px;margin-right:53px">
+		<p><b>APRIL</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td></td>
+                        <td></td>
+						<td></td>
+						<td></td>
+						<td><a href="#41">1</a></td>
+						<!--<td><a href=""></a></td>-->
+                     </tr>
+                     <tr>
+                        <td><a href="#44">4</a></td>
+                        <td><a href="#45">5</a></td>
+						<td><a href="#46">6</a></td>
+						<td><a href="#47">7</a></td>
+						<td><a href="#48">8</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#411">11</a></td>
+                        <td><a href="#412">12</a></td>
+						<td><a href="#413">13</a></td>
+						<td><a href="#414">14</a></td>
+						<td><a href="#415">15</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#418">18</a></td>
+                        <td><a href="#419">19</a></td>
+						<td><a href="#420">20</a></td>
+						<td><a href="#421">21</a></td>
+						<td><a href="#422">22</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#425">25</a></td>
+                        <td><a href="#426">26</a></td>
+						<td><a href="#427">27</a></td>
+						<td><a href="#428">28</a></td>
+						<td><a href="#429">29</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+		<div id="centro" style="text-align:center;margin-left:82px">
+		<p><b>MAY</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td><a href="#52">2</a></td>
+                        <td><a href="#53">3</a></td>
+						<td><a href="#54">4</a></td>
+						<td><a href="#55">5</a></td>
+						<td><a href="#56">6</a></td>
+						<!--<td><a href=""></a></td>-->
+                     </tr>
+                     <tr>
+                        <td><a href="#59">9</a></td>
+                        <td><a href="#510">10</a></td>
+						<td><a href="#511">11</a></td>
+						<td><a href="#512">12</a></td>
+						<td><a href="#513">13</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#516">16</a></td>
+                        <td><a href="#517">17</a></td>
+						<td><a href="#518">18</a></td>
+						<td><a href="#519">19</a></td>
+						<td><a href="#520">20</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#523">23</a></td>
+                        <td><a href="#524">24</a></td>
+						<td><a href="#525">25</a></td>
+						<td><a href="#526">26</a></td>
+						<td><a href="#527">27</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#530">30</a></td>
+                        <td><a href="#5">31</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+		<div id="derecha" style="text-align:center;margin-left:135px;margin-right:55px">
+		<p><b>JUNE</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td></td>
+                        <td></td>
+						<td><a href="#61">1</a></td>
+						<td><a href="#62">2</a></td>
+						<td><a href="#63">3</a></td>
+						<!--<td><a href=""></a></td>-->
+                     </tr>
+                     <tr>
+                        <td><a href="#66">6</a></td>
+                        <td><a href="#67">7</a></td>
+						<td><a href="#68">8</a></td>
+						<td><a href="#69">9</a></td>
+						<td><a href="#610">10</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#613">13</a></td>
+                        <td><a href="#614">14</a></td>
+						<td><a href="#615">15</a></td>
+						<td><a href="#616">16</a></td>
+						<td><a href="#617">17</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#620">20</a></td>
+                        <td><a href="#621">21</a></td>
+						<td><a href="#622">22</a></td>
+						<td><a href="#623">23</a></td>
+						<td><a href="#624">24</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#627">27</a></td>
+                        <td><a href="#628">28</a></td>
+						<td><a href="#629">29</a></td>
+						<td><a href="#630">30</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+	</div>
+	<div id="cuerpo" style="height:200px">
+		<div id="izquierda" style="text-align:center;margin-left:55px;margin-right:53px">
+		<p><b>JULY</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td></td>
+                        <td></td>
+						<td></td>
+						<td></td>
+						<td><a href="#71">1</a></td>
+						<!--<td><a href=""></a></td>-->
+                     </tr>
+                     <tr>
+                        <td><a href="#74">4</a></td>
+                        <td><a href="#75">5</a></td>
+						<td><a href="#76">6</a></td>
+						<td><a href="#77">7</a></td>
+						<td><a href="#78">8</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#712">11</a></td>
+                        <td><a href="#712">12</a></td>
+						<td><a href="#713">13</a></td>
+						<td><a href="#714">14</a></td>
+						<td><a href="#715">15</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#718">18</a></td>
+                        <td><a href="#719">19</a></td>
+						<td><a href="#720">20</a></td>
+						<td><a href="#721">21</a></td>
+						<td><a href="#722">22</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#725">25</a></td>
+                        <td><a href="#726">26</a></td>
+						<td><a href="#727">27</a></td>
+						<td><a href="#728">28</a></td>
+						<td><a href="#729">29</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+		<div id="centro" style="text-align:center;margin-left:82px">
+		<p><b>AUGUST</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td><a href="#81">1</a></td>
+                        <td><a href="#82">2</a></td>
+						<td><a href="#83">3</a></td>
+						<td><a href="#84">4</a></td>
+						<td><a href="#85">5</a></td>
+						<!--<td><a href=""></a></td>-->
+                     </tr>
+                     <tr>
+                        <td><a href="#88">8</a></td>
+                        <td><a href="#89">9</a></td>
+						<td><a href="#810">10</a></td>
+						<td><a href="#811">11</a></td>
+						<td><a href="#812">12</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#815">15</a></td>
+                        <td><a href="#816">16</a></td>
+						<td><a href="#817">17</a></td>
+						<td><a href="#818">18</a></td>
+						<td><a href="#819">19</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#822">22</a></td>
+                        <td><a href="#823">23</a></td>
+						<td><a href="#824">24</a></td>
+						<td><a href="#825">25</a></td>
+						<td><a href="#826">26</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#829">29</a></td>
+                        <td><a href="#830">30</a></td>
+						<td><a href="#831">31</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+		<div id="derecha" style="text-align:center;margin-left:135px;margin-right:55px">
+		<p><b>SEPTEMBER</b></p>
+            <table border class="striped" border="1" style="margin:0 auto" >
+                 <thead bgcolor="#A9F5F2">
+					<tr>
+                        <th>Monday</th>
+                        <th>Tuesday</th>
+                        <th>Wednesday</th>
+						<th>Thursday</th>
+						<th>Friday</th>
+					</tr>
+                 </thead>
+                 <tbody>
+                     <tr>
+						<td></td>
+                        <td></td>
+						<td></td>
+						<td><a href="#91">1</a></td>
+						<td><a href="#92">2</a></td>
+						<!--<td><a href=""></a></td>-->
+                     </tr>
+                     <tr>
+                        <td><a href="#95">5</a></td>
+                        <td><a href="#96">6</a></td>
+						<td><a href="#97">7</a></td>
+						<td><a href="#98">8</a></td>
+						<td><a href="#99">9</a></td>
+                     </tr>
+					<tr>
+                        <td><a href="#912">12</a></td>
+                        <td><a href="#913">13</a></td>
+						<td><a href="#914">14</a></td>
+						<td><a href="#915">15</a></td>
+						<td><a href="#916">16</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#919">19</a></td>
+                        <td><a href="#920">20</a></td>
+						<td><a href="#921">21</a></td>
+						<td><a href="#922">22</a></td>
+						<td><a href="#923">23</a></td>
+                     </tr>
+					 <tr>
+                        <td><a href="#926">26</a></td>
+                        <td><a href="#927">27</a></td>
+						<td><a href="#928">28</a></td>
+						<td><a href="#929">29</a></td>
+						<td><a href="#930">30</a></td>
+                     </tr>
+                 </tbody>
+             </table>
+		</div>
+	</div>
+	  <div class="hr-divider"></div><a name="11"></a>
+	  <h2 style="text-align:center"><b>JANUARY</b></h2>
+<div id="january">
+ <div class="container">
+	<h3 style="color:#04B404"><b>1<sup>st</sup> January</b></h3>
+	<p style="font-size:18px"></p><a name="14"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>4<sup>nd</sup> January</b></h3>
+	<p style="font-size:18px"></p><a name="15"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>5<sup>th</sup> January</b></h3>
+	<p style="font-size:18px"></p><a name="16"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>6<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Prepare plates with LB+Km and plate the strain with pSB1K3 (E. coli DH5α)</p><a name="17"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>7<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Inocula of pSB1K3 in 15 mL of LB+Km (kanamycin)</p>
+	<a name="18"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>8<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Minipreps of pSB1K3</p>
+	<p style="font-size:18px">Plate the strains of <i>E. coli</i> DH5α with pMPO364 (<i>nasF</i> and <i>nahR-</i>P<i>sal</i>), JB3Tc19::PleD (<i>pleD*</i>), pMPO52 (P<i>m</i>) and pMRB11(<i>yhjH</i>), and P. putida KT2442 (<i>lapG</i>)</p>
+	<a name="111"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>11<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Put one colony of each strain in 3 mL of LB+specific antibiotic and incubate overnight</p><a name="112"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>12<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Minipreps and measure the concentration of the cultures</p><a name="113"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>13<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">14 first primers have arrived. Resuspend them, dilute until 10 μM and storage at -20ºC</p>
+	<p style="font-size:18px">PCR of pMPO364 and pMRB11 [45ºC]</p>
+	<p style="font-size:18px">PCR of pJBeTc19::pleD and <i>Pseudomonas putida</i> KT2442 chromosome [60ºC]</p>
+	<p style="font-size:18px">Electrophoresis of both PCR</p>
+	<a name="114"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>14<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Repeat the PCR at 45ºC and at 60ºC</p>
+	<p style="font-size:18px">Electrophoresis of both PCR</p>
+	<p style="font-size:18px">Purify the 5 PCR products (<i>lapG, nahR-</i>P<i>sal, nasF, pleD*, yhjH</i>) and store them at -20ºC</p>
+	<a name="115"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>15<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Digest the purified PCR products and pSB1K3 where we will clone the PCR products</p>
+	<p style="font-size:18px">Electrophoresis of the digested PCR products and vector and cut a slice of the gel that contain the fragment of interest and purify</p>
+	<p style="font-size:18px">Plate the strain with pMPO1035 (<i>xylS2</i>)</p>
+	<a name="118"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>18<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Ligation the vector with each PCR product</p>
+	<p style="font-size:18px">Put one colony of pMPO1035 in 3 mL of LB+Cm (Chloramphenicol)</p>
+	<a name="119"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>19<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Transformation of competent cells, E. coli DH5α, with the ligations</p>
+	<p style="font-size:18px">Miniprep and measure the concentration of pMPO1035</p>
+	<a name="120"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>20<sup>th</sup> January</b></h3>
+	<p style="font-size:18px"> </p>
+	<p style="font-size:18px">Inocula of each transformation event in 3 mL of LB+Km</p>
+	<a name="121">PCR of pMPO1035</a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>21<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Miniprep the plasmid DNA of the transformations</p>
+	<p style="font-size:18px">Diagnostic digest the plasmids with PstI and XbaI</p>
+	<p style="font-size:18px">Electrophoresis of the digestions and the PCR. All the fragments had the correct length</p>
+	<p style="font-size:18px">Purify the PCR of <i>xylS2</i> and digest with PstI and XbaI</p>
+	<p style="font-size:18px">Purify the digestion of <i>xylS2</i> and ligation with pSB1K3</p>
+	<a name="122"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>22<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Transformation of competent cells, E. coli DH5α, with the ligation</p>
+	<p style="font-size:18px"></p>
+	<a name="125"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>25<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Repeat the ligation of xylS2 with the vector, because the plates were contaminated</p>
+	<a name="126"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>26<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Send to sequence 4 constructions (<i>lapG, nahR-</i>P<i>sal, nasF, yhjH</i>)</p>
+	<p style="font-size:18px">Transformation of competent cells, <i>E. coli</i> DH5α, with the ligation</p>
+	<a name="127"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>27<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">The transformation was unsuccessful, so we did a electrophoresis of the vector and the insert (xylS2) to test that we have DNA in the sample</p>
+	<p style="font-size:18px">Repeat the ligation of xylS2 with the vector</p>
+	<p style="font-size:18px">Dilute until 10 μM the primers that we use to do a overlap extension PCR</p>
+	<a name="128"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>28<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Test the received sequences of <i>lapG, nahR-</i>P<i>sal, nasF</i> and <i>yhjH</i>. All of them were correct</p>
+	<p style="font-size:18px">Transformation of competent cells, E. coli DH5α, with the ligation and the tested constructions. Now we name these constructions as pMRB120 (<i>nahR-</i>P<i>sal</i>), pMRB121 (<i>lapG</i>), pMRB122 (<i>yhjH</i>) and pMRB123 (<i>nasF</i>)</p>
+	<p style="font-size:18px">Digest pMRB121, pMRB122 and pMRB123 as vector and pMRB120 as insert</p>
+	<a name="129"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> January</b></h3>
+	<p style="font-size:18px">Electrophoresis of the digestions and cut a slice that contains the fragment of interest and purify</p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+  </div>
+ 	  <div class="hr-divider"></div><a name="21"></a>
+	  <h2 style="text-align:center"><b>FEBRUARY</b></h2>
+ <div id="february">
+ <div class="container">
+	<h3 style="color:#04B404"><b>1<sup>st</sup> February</b></h3>
+	<p style="font-size:18px">Inocula of each transformation and xylS2 in 3 mL of LB+Km</p>
+	<p style="font-size:18px">Ligation of <i>yhjH, lapG</i> and <i>nasF</i> with <i>nahR-</i>P<i>sal</i></p>
+	<a name="22"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>2<sup>nd</sup> February</b></h3>
+	<p style="font-size:18px">Miniprep and diagnostic digestion of xylS2</p>
+	<p style="font-size:18px">Storage the pMRB120, pMRB121, pMRB122 and pMRB123 strains at -80ºC</p>
+	<p style="font-size:18px">Transformation of competent cells, E. coli DH5α, with the ligations</p>
+	<p style="font-size:18px">Send to sequence the constructions of xylS2 and pleD*</p>
+	<a name="23"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>3<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Take biomass of the transformations and plate onto other plate, because there were too many cells on the plates</p>
+	<p style="font-size:18px">First part of overlap extension PCR of P<i>m</i></p>
+	<a name="24"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>4<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Inocula of the transformation in 3 mL of LB+Km</p>
+	<p style="font-size:18px">Electrophoresis of PCR of <i>P</i>m, but it did not go well</p>
+	<p style="font-size:18px">Repeat the PCR of <i>P</i>m</p>
+	<a name="25"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>5<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Miniprep of <i>nahR-</i>P<i>sal-lapG, nahR-</i>P<i>sal-yhjH</i> and <i>nahR-</i>P<i>sal-nasF</i></p>
+	<p style="font-size:18px">Diagnostic digest of these plasmids</p>
+	<p style="font-size:18px">Test the received sequences of <i>xylS2</i> and <i>pleD*</i>. Only <i>xylS2</i> is correct, so do a transformation to introduce the plasmid, now we name this construction as pMRB124</p>
+	<a name="28"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>8<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Inocula of <i>xylS2</i> strain in LB+Km</p>
+	<p style="font-size:18px">Transformation with nahR-Psal-yhjH V, nahR-Psal-lapG IV and nahR-Psal-nasF II. Now, we name the constructions as pMRB125, pMRB126 and pMRB127, respectively</p>
+	<p style="font-size:18px">Electrophoresis of PCR product, but only P<i>m</i> RM 1 (the fragment of P<i>m</i> amplified with the primer reverse and mutagenic forward 1) did not go well</p>
+	<a name="29"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>9<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Inocula of each transformation in LB+Km</p>
+	<p style="font-size:18px">Storage the pMRB124 strain at -80ºC</p>
+	<p style="font-size:18px">Repeat the PCR of P<i>m</i> RM 1</p>
+	<p style="font-size:18px">Electrophoresis of the PCR</p>
+	<a name="210"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>10<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Storage the pMRB125, pRMB126 and pMRB127 strains at -80ºC</p>
+	<p style="font-size:18px">Purify all the PCR products of P<i>m</i></p>
+	<p style="font-size:18px">Digest pMRB127 with EcoRI and SpeI as insert</p>
+	<p style="font-size:18px">Ligation of the vector with <i>pleD*</i>, and <i>lapG</i> and <i>yhjH</i> with <i>nahR-</i>P<i>sal-nasF</i></p>
+	<a name="211"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>11<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Electrophoresis of the digestion of pMRB127, cut the slice that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Transformation with the ligations</p>
+	<p style="font-size:18px">Overlap extension PCR of P<i>m</i>1, P<i>m</i>2 and P<i>m</i>3, and PCR of P<i>m</i> RM1 and P<i>m</i> (In)</p>
+	<p style="font-size:18px">Electrophoresis of the overlap extension PCR</p>
+	<a name="212"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>12<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Electrophoresis of P<i>m</i> RM1 and P<i>m</i> FM1 to compare the concentrations. Dilute 10 times and repeat the overlap extension PCR</p>
+	<p style="font-size:18px">Electrophoresis of the overlap extension PCR of P<i>m</i>1. It does not go well</p>
+	<a name="215"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>15<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Inocula of <i>nahR-</i>P<i>sal-nasF-lapG</i> and  <i>nahR-</i>P<i>sal-nasF-yhjH</i> (complex devices), pUC18Sfi;miniTn7BBGm and <i>pleD*</i> in LB</p>
+	<p style="font-size:18px"></p>
+	<p style="font-size:18px">Repeat overlap extension PCR of P<i>m</i>1</p>
+	<a name="216"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>16<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Miniprepion of the plasmid DNA of the cultures</p>
+	<p style="font-size:18px">Diagnostic digestion of <i>pleD*</i> and complex devices</p>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestions and the PCR of P<i>m</i>1</p>
+	<a name="217"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>17<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Transformation with pleD* and complex devices. Now, we name the constructions as pMRB145, pMRB129 and pMRB130, respectively</p>
+	<p style="font-size:18px">Digest Tn7 and the vector as vector and P<i>m</i>, P<i>m</i>1, P<i>m</i>2, P<i>m</i>3, <i>nahR-</i>P<i>sal-nasF-lapG</i> and <i>nahR-</i>P<i>sal-nasF-yhjH</i></p>
+	<p style="font-size:18px">Ligation of the vector with P<i>m</i>, P<i>m</i>1, P<i>m</i>2 and P<i>m</i>3 (P<i>m</i> vectors), and Tn7 with  <i>nahR-</i>P<i>sal-nasF-lapG</i> and <i>nahR-</i>P<i>sal-nasF-yhjH</i> (Tn7 devices)</p>
+	<a name="218"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>18<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Inocula of each transformation in LB+Km</p>
+	<p style="font-size:18px">Transformation with the ligations</p>
+	<a name="219"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>19<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Miniprep of pleD* and complex devices</p>
+	<p style="font-size:18px">Storage pMRB145, pMRB129 and pMRB130 at -80ºC</p>
+	<a name="222"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>22<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Incocula of each transformation in LB</p>
+	<p style="font-size:18px">Plate the strains with pMRB124 (<i>xylS2</i>) and pTNS2 (helper of Tn7)</p>
+	<p style="font-size:18px">Digest pMRB129 and pMRB130 as insert</p>
+	<a name="223"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>23<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Miniprep of the cultures</p>
+	<p style="font-size:18px">Electrophoresis of the digestions, cut a slice that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Inocula of 124 and pTNS2 in 3 mL of LB</p>
+	<a name="224"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>24<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Diagnostic digestion of P<i>m</i> vectors and Tn7 devices</p>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestions of P<i>m</i>  vectors and Tn7 devices</p>
+	<p style="font-size:18px">Miniprep of the cultures of 124 and pTNS2</p>
+	<p style="font-size:18px">Send to sequence the constructions of P<i>m</i></p>
+	<a name="225"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>25<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Repeat the transformation with Tn7 devices. Now, we name the constructions as pMRB133 (Tn7-<i>nahR-</i>P<i>sal-nasF-lapG</i>) and pMRB134 (Tn7-<i>nahR-</i>P<i>sal-nasF-yhjH</i>)</p>
+	<p style="font-size:18px">Digest 124, 120 and pSB1K3 as vector</p>
+	<p style="font-size:18px">Ligation of 124 (v) with 120 (i) and 127 (i)</p>
+	<p style="font-size:18px">Inocula of pMRB125 and pMRB126 in 3 mL of LB</p>
+	<a name="226"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>26<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Segregate the plates of 133 and 134</p>
+	<p style="font-size:18px">Miniprep of the cultures of 125 and 126</p>
+	<p style="font-size:18px">Heat-shock transformation with the ligations</p>
+	<p style="font-size:18px">Digest 125 and 126 as insert</p>
+	<a name="229"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> February</b></h3>
+	<p style="font-size:18px">Inocula of 133 and 134 in 5 mL of LB and 124+120 in 3 mL of LB</p>
+	<p style="font-size:18px">Segregate the plate of 124+127</p>
+	<p style="font-size:18px">Heat-shock transformation with the constructions of P<i>m</i>. Now, we name the constructions as pMRB137 (P<i>m</i>), pMRB138 (P<i>m</i>1), pMRB139 (P<i>m</i>2) and pMRB140 (P<i>m</i>3)</p>
+	<p style="font-size:18px">Electrophoresis of the digestions of 125 and 126, cut a slice of gel that contains the fragment of interest and purify</p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+  </div>
+ <div class="hr-divider"></div><a name="31"></a>
+	  <h2 style="text-align:center"><b>MARCH</b></h2>
+ <div id="march">
+ <div class="container">
+	<h3 style="color:#04B404"><b>1<sup>st</sup> March</b></h3>
+	<p style="font-size:18px">Miniprep of the cultures</p>
+	<p style="font-size:18px">Storage pMRB133 and pMRB134 at -80ºC</p>
+	<p style="font-size:18px">Inocula of 124+127 in 3 mL of LB, and each construction of Pm in 5 mL of LB</p>
+	<a name="32"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>2<sup>nd</sup> March</b></h3>
+	<p style="font-size:18px">Miniprep of the culture</p>
+	<p style="font-size:18px">Segregate the plates of the transformations</p>
+	<p style="font-size:18px">Digest and electrophoresis of 124+127</p>
+	<p style="font-size:18px">Ligation Tn7 with 125 (i) and 126 (i)</p>
+	<a name="33"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>3<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Storage pMRB137, pMRB138, pMRB139 and pMRB140 at -80ºC</p>
+	<p style="font-size:18px">Transformation of the ligations and pleD*. Now, we name the construction of pleD* as pMRB145</p>
+	<p style="font-size:18px">Digest 124 as vector</p>
+	<p style="font-size:18px">Electrophoresis of the digestion, cut a slice of gel that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Ligation 124 (v) with 120 (i) and 127 (i), and Tn7 with 125 (i) and 126 (i)</p>
+	<a name="34"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>4<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Segregate the plate of the transformation</p>
+	<p style="font-size:18px">Heat-shock transformation with the ligations</p>
+	<p style="font-size:18px">Plate the strain with pMRB1 (vector that contains the protein fusion gen <i>lapZ-gfp</i>mut3)</p>
+	<a name="37"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>7<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Inocula of 145 in 5 mL of LB</p>
+	<p style="font-size:18px">Inocula of pMRB1,124+120 and 124+127 in 3 mL of LB
+	<a name="38"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>8<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Storage pMRB145 at -80ºC</p>
+	<p style="font-size:18px">Miniprep of the cultures</p>
+	<p style="font-size:18px">Digest 145 as vector and 137, 138, 139 and 140 as insert</p>
+	<p style="font-size:18px">Electrophoresis of the digestions, cut a slice of gel that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Diagnostic digestion of the pMRB124 constructions</p>
+	<a name="39"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>9<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestions</p>
+	<p style="font-size:18px">Digest pMRB1 as vector</p>
+	<p style="font-size:18px">Repeat the ligation of Tn7 with 125 (i) and 126 (i), and 145 (v) with 120 (i) and 127 (i)</p>
+	<p style="font-size:18px">Segregate the plate of 124+127. Now, we name this construction as pMRB146</p>
+	<a name="310"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>10<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Transformation with the ligations</p>
+	<p style="font-size:18px">Electrophoresis of the digestion of pMRB1 (v), cut a slice of gel that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Ligation of pMRB1 (v) with the four P<i>m</i> (i)</p>
+	<a name="311"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>11<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Transformation with the ligations</p>
+	<p style="font-size:18px">Plate the strain with 128</p>
+	<p style="font-size:18px">Digest of pMRB125 and pMRB126 as insert</p>
+	<a name="314"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>14<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Inocula of 145+120, 145+127, P<i>m</i> contructions, 128 and 146</p>
+	<p style="font-size:18px">Diagnostic digestion of 145+120 and 145+127</p>
+	<p style="font-size:18px">Ligation of Tn7 with 120 (i) and 127 (i), and 124 (v) with 120 (i)</p>
+	<a name="315"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>15<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Miniprep of the cultures and 128 digest as vector</p>
+	<p style="font-size:18px">Diagnostic digestion of the P<i>m</i> constructions</p>
+	<p style="font-size:18px">Storage pMRB146 at -80ºC</p>
+	<p style="font-size:18px">Transformation with the ligations</p>
+	<a name="316"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>16<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestions</p>
+	<p style="font-size:18px">Electrophoresis of the digestions of 125 (i) and 126 (i), cut a slice of gel that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Ligation of Tn7 with 120, 125 and 126, and 145 (v) with 120 (i)</p>
+	<p style="font-size:18px">Inocula of Tn7+127 and 124+120</p>
+	<a name="317"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>17<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Miniprep of the cultures</p>
+	<p style="font-size:18px">Diagnostic digestion of 124+120 and Tn7+127</p>
+	<p style="font-size:18px">Transformation with the ligations</p>
+	<a name="318"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>18<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">The transformations with Tn7 devices didn't work, so plate Tn7 strain again</p>
+	<p style="font-size:18px">Transformation with 145+127. Now, we name this construction as pMRB147</p>
+	<a name="321"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>21<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Inocula of Tn7, 147, 145+120 and 145+127</p>
+	<p style="font-size:18px">Repeat the ligation of pMRB1 (v) with 137 (i)</p>
+	<a name="322"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>22<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Miniprep of the cultures</p>
+	<p style="font-size:18px">Diagnostic digestion of 145+127 and 145+120</p>
+	<p style="font-size:18px">Electrophoresis of pMRB128 (v), cut a slice of gel that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Transformation with pMRB1+137 and Tn7+127</p>
+	<p style="font-size:18px">Ligation of Tn7 (v) with 125 (i)</p>
+	<a name="323"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>23<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Inocula of pMRB1+137</p>
+	<p style="font-size:18px">Transformation with Tn7+pMRB125</p>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestion of 145+120</p>
+	<a name="324"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>24<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Minipreps and diagnostic digestion of 1+137</p>
+	<p style="font-size:18px">Segregate the plate of Tn7+127</p>
+	<p style="font-size:18px">Digestion of 145 and 147 as an insert</p>
+	<p style="font-size:18px">Ligation of Tn7 (v) with 125 (i) and 126 (i), and 145 (v) with 120 (i)</p>
+	<a name="325"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>25<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Transformation of the ligations</p>
+	<p style="font-size:18px">Electrophoresis of the digestions, cut a slice of gel that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Diagnostic digestion of pMRB1+137/138/139/140</p>
+	<a name="328"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>28<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Inocula of Tn7+127. Now, we name this construction as pMRB151</p>
+	<p style="font-size:18px">The transformations did not go well</p>
+	<a name="329"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Ligation of 120 (v) with 124 (i), Tn7 (v) with 125 (i), 147 (i) and 126 (i), pMRB1 (v) with 137-140 (i) and 145 (v) with 120 (i)</p>
+	<p style="font-size:18px">Store at -80ºC and minipreps of pMRB151</p>
+	<a name="330"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>30<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Transformation of the ligations</p>
+	<a name="331"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>31<sup>th</sup> March</b></h3>
+	<p style="font-size:18px">Inocula of 1+137/138/139/140, 120+145,120+124 and Tn7+147</p>
+	<p style="font-size:18px">Segregate the transformation of Tn7+126</p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+  </div>
+  <div class="hr-divider"></div><a name="41"></a>
+  <h2 style="text-align:center"><b>APRIL</b></h2>
+ <div id="april">
+ <div class="container">
+	<h3 style="color:#04B404"><b>1<sup>st</sup> April</b></h3>
+	<p style="font-size:18px">Minipreps and diagnostic digestion of the 1+137/138/139/140, 120+124, 145+120 and Tn7+147</p>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestion</p>
+	<a name="44"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>4<sup>nd</sup> April</b></h3>
+	<p style="font-size:18px">2nd diagnostic digestions of 1+137/138/139/140</p>
+	<p style="font-size:18px">Inocula of 124,135 and 136</p>
+	<p style="font-size:18px">Transformation of 145+120 and Tn7+147. Now, we name these constructions as pMRB135 and pMRB136, respectively</p>
+	<a name="45"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>5<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Storage pMRB135 and pMRB136 at -80ºC</p>
+	<p style="font-size:18px">Inocula of KT2442</p>
+	<p style="font-size:18px">Digest 135,145 and 130 as insert and Tn7 as vector</p>
+	<p style="font-size:18px">Measure the concentration of pTNS2, pMRB136 and pMRB134></p>
+	<a name="46"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>6<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Electrophoresis of digestion of 135, 145, 130 and Tn7, cut a slice of gel that contains the fragment of interest and purify</p>
+	<p style="font-size:18px">Plate the strains with pMRB136 and pTNS2</p>
+	<p style="font-size:18px">Electroporation of KT2442 with 136 and 134</p>
+	<a name="47"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>7<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Inocula of pTNS2 and 136</p>
+	<p style="font-size:18px">Ligation of Tn7 with 126 and 135</p>
+	<p style="font-size:18px">Colony PCR of the electroporations</p>
+	<a name="48"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>8<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Minipreps of pTNS2 and 136</p>
+	<p style="font-size:18px">Transformation of the ligations</p>
+	<p style="font-size:18px">Electrophoresis of the colony PCR</p>
+	<a name="411"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>11<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Inocula of Tn7+126 and Tn7+135</p>
+	<p style="font-size:18px">Ligation of 1 (v) with 138 (i), 124 (v) with 120 (i) and Tn7 (v) with 120 (i) and 125 (i)</p>
+	<a name="412"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>12<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Minipreps and diagnostic digestion of the cultures</p>
+	<p style="font-size:18px">Transformation of the ligations</p>
+	<a name="413"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>13<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Inocula of 1+138, 124+120, and Tn7+125. Now ,we name 124+120 as pMRB152</p>
+	<p style="font-size:18px">Ligation of Tn7 (v) with 130 (i), 145 (i), 146 (i) and 120 (i)</p>
+	<a name="414"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>14<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Minipreps of the cultures</p>
+	<p style="font-size:18px">Storage pMRB152 at -80ºC</p>
+	<p style="font-size:18px">Diagnostic digestion and electrophoresis of 1+137/138/139/140, Tn7+125, Tn7+135 and Tn7+126</p>
+	<p style="font-size:18px">Transformation with Tn7+120, Tn7+145, Tn7+130, Tn7+146 and Tn7+125</p>
+	<a name="415"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>15<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Measure the concentration of 136 and 134</p>
+	<p style="font-size:18px">Diagnostic digestions of 1+138, Tn7+126 and Tn7+135</p>
+	<p style="font-size:18px">Plate the strain with pTNS2</p>
+	<a name="418"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>18<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Inocula of KT2442, pTNS2, Tn7+145, Tn7+130, Tn7+146, Tn7+120 and Tn7+125. Now, we name Tn7+125 as pMRB128, Tn7+130 as pMRB164 and Tn7+145 as pMRB165</p>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestions</p>
+	<a name="419"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>19<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Electroporation of KT2442 with 164, 165, 134 and 136</p>
+	<p style="font-size:18px">Minipreps of pTNS2, Tn7+146 and Tn7+120</p>
+	<p style="font-size:18px">Storage pMRB128, pMRB164 and pMRB165 at -80ºC</p>
+	<p style="font-size:18px">Transformation of 1+Pms. Now, we name 1+Pm, 1+Pm1, 1+Pm2 and 1+Pm3 as pMRB166, pMRB167, pMRB168 and pMRB169</p>
+	<a name="420"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>20<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Colony PCR of the electroporations and electrophoresis</p>
+	<p style="font-size:18px">Diagnostic digestion of Tn7+146 and Tn7+120</p>
+	<p style="font-size:18px">Segregate the positive candidates of electroporations</p>
+	<p style="font-size:18px">Plate KT2442-Tn7</p>
+	<p style="font-size:18px">Inocula of 166, 167, 168 and 169</p>
+	<a name="421"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>21<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Inocula of KT2442-Tn7, KT2442-T134, KT2442-T136, KT2442-T164 and KT2442-T165</p>
+	<p style="font-size:18px">Electrophoresis of Tn7+146 and Tn7+120</p>
+	<p style="font-size:18px">Diagnostic digestion of 120 and 146</p>
+	<p style="font-size:18px">Storage at -80ºC and minipreps of pMRB166-169</p>
+	<a name="422"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>22<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Digest 152 as insert</p>
+	<p style="font-size:18px">Electroporation of KT2442-T134, KT2442-T136, KT2442-T164 and KT2442-T165 with pCdrA</p>
+	<a name="425"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>25<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Plate the strains KT2442-Tn7, KT2442-T134, KT2442-T136, KT2442-T164 and KT2442-T165</p>
+	<p style="font-size:18px">Inocula for fluorimetry</p>
+	<p style="font-size:18px">Electrophoresis of 152 (i), cut a slice of gel that contains the fragment of interest and purify</p>
+	<a name="426"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>26<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Digest pSB1K3 as vector</p>
+	<p style="font-size:18px">Inocula of KT2442-Tn7, KT2442-T134, KT2442-T136, KT2442-T164 and KT2442-T165</p>
+	<p style="font-size:18px">Fluorimeter</p>
+	<a name="427"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>27<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">INSTANT curves of growth and biofilm of KT2442-Tn7/T134/T136/T164/T165</p>
+	<p style="font-size:18px">Failed fluorimeter</p>
+	<a name="428"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>28<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Dye and measure the plates of INSTANT curves</p>
+	<p style="font-size:18px">Digest 120 as insert</p>
+	<p style="font-size:18px">Ligation Tn7+146 and Tn7+152</p>
+	<a name="429"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> April</b></h3>
+	<p style="font-size:18px">Transformation of the ligations</p>
+	<p style="font-size:18px">Plate 128, 133, KT2442-Tn7/T136/T165 and KT2442, KT2442 <i>lapG</i><sup>-</sup> and KT2442 ΔbifA</p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+  </div>
+ <div class="hr-divider"></div><a name="52"></a>
+	  <h2 style="text-align:center"><b>MAY</b></h2>
+ <div id="may">
+ <div class="container">
+	<h3 style="color:#04B404"><b>2<sup>st</sup> May</b></h3>
+	<p style="font-size:18px">Inocula of Tn7+146, Tn7+152, KT2442 <i>lapG</i><sup>-</sup>, 128 and 133</p>
+	<p style="font-size:18px">Plate all the KT2442 with pCdrA, KT2442 ΔfleQ and all the variants of KT2442 of pleD*</p>
+	<p style="font-size:18px">Electrophoresis of 120 (i), cut a slice of gel that contains the fragment of interest and purify</p>
+	<a name="53"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>3<sup>nd</sup> May</b></h3>
+	<p style="font-size:18px">Minipreps and measure the concentration of 128 and 133</p>
+	<p style="font-size:18px">Minipreps and diagnostic digestion of Tn7+146 and Tn7+152</p>
+	<p style="font-size:18px">Electroporation of KT2442 <i>lapG</i><sup>-</sup> with 128 and 133</p>
+	<a name="54"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>4<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Inocula for curves and fluorimeter</p>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestions</p>
+	<p style="font-size:18px">Dilutions of fluorimeter and curves of KTs</p>
+	<a name="55"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>5<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Dye and measure the curves of pleD*</p>
+	<p style="font-size:18px">Take the data of the fluorimeter</p>
+	<a name="56"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>6<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Plate the variants of KT2442 with yhjH</p>
+	<p style="font-size:18px">Transformation of Tn7+120</p>
+	<a name="59"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>9<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Inocula of KT2442-Tn7/T134/T164 and Tn7+120</p>
+	<p style="font-size:18px">Transformation with Tn7+146 and Tn7+152. Now, we name these strains as pMRB159 and pMRB170, respectively</p>
+	<a name="510"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>10<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Minipreps and diagnostic digestion of Tn7+120</p>
+	<p style="font-size:18px">Dilutions of <i>yhjH</i> and put the curves for 20 h</p>
+	<p style="font-size:18px">Segregate KT2442 <i>lapG</i><sup>-</sup>-T128/T133</p>
+	<a name="511"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>11<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Dye and measure the plates of <i>yhjH</i></p>
+	<p style="font-size:18px">Inocula of 159 and 170</p>
+	<p style="font-size:18px">Electrophoresis of the diagnostic digestion</p>
+	<p style="font-size:18px">Colony PCR of KT2442 <i>lapG</i><sup>-</sup>-T128/T133</p>
+	<p style="font-size:18px">Transformation with Tn7+120</p>
+	<a name="512"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>12<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Minipreps of the cultures</p>
+	<p style="font-size:18px">Storage pMRB159 and pMRB170 at -80ºC</p>
+	<p style="font-size:18px">Inocula of Tn7+120</p>
+	<a name="513"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>13<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">PCR of pMRB1 to get the gene <i>gfp</i></p>
+	<p style="font-size:18px">Electrophoresis of the colony PCRs</p>
+	<p style="font-size:18px">Segregate Tn7+120</p>
+	<a name="516"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>16<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Inocula of pleD* constructions in Tn7 and KT2442 <i>lapG</i><sup>-</sup>-T128</p>
+	<p style="font-size:18px">Inocula of Tn7+120</p>
+	<p style="font-size:18px">Colony PCR and electrophoresis of KT2442 <i>lapG</i><sup>-</sup>-T133</p>
+	<a name="517"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>17<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Storage KT2442 <i>lapG</i><sup>-</sup>-T128 at -80ºC</p>
+	<p style="font-size:18px">Dilutions of pleD* curves</p>
+	<p style="font-size:18px">Plate KT2442 <i>lapG</i><sup>-</sup> and KT2442 ΔbifA</p>
+	<p style="font-size:18px">Inocula of KT2442</p>
+	<p style="font-size:18px">Inocula of KT2442 and KT2442-Tn7/T134/T136/T164/T165 for Congo Red and curves</p>
+	<a name="518"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>18<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Dye and measure the pleD* plates</p>
+	<p style="font-size:18px">Plate the strains with 134, 136, 164, 170, 159 and Tn7</p>
+	<p style="font-size:18px">Inocula of KT2442-Tn7/T165 for curves</p>
+	<p style="font-size:18px">Inocula of KT2442 <i>lapG</i><sup>-</sup> and KT2442 ΔbifA</p>
+	<p style="font-size:18px">Plates of Congo Red</p>
+	<p style="font-size:18px">Curves of 134, 164 and KT2442</p>
+	<a name="519"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>19<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Electrophoresis of the PCR of <i>gfp</i></p>
+	<p style="font-size:18px">Curves of 165</p>
+	<p style="font-size:18px">Electroporation of KT2442 <i>lapG</i><sup>-</sup> and ΔbifA with Tn7, 128 and 133</p>
+	<p style="font-size:18px">Dye and measure the plates of 134, 164 and KT2442</p>
+	<p style="font-size:18px">Let more time the plates with Congo Red</p>
+	<a name="520"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>20<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Dye and measure the plates of 165 at 17 h and 20 h</p>
+	<p style="font-size:18px">Take a photo of the plates with Congo Red</p>
+	<p style="font-size:18px">Let the plates with Congo Red at RT overnight</p>
+	<a name="523"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>23<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Inocula of 170, 159 and Tn7</p>
+	<p style="font-size:18px">Electroporation of KT2442 with 159, 170, 128 and 133</p>
+	<p style="font-size:18px">Minipreps and measure the concentration of 170, 159 and Tn7</p>
+	<p style="font-size:18px">Digestion of 164 and 148</p>
+	<p style="font-size:18px">PCR of <i>gfp</i></p>
+	<p style="font-size:18px">Electrophoresis of the digestions and PCR</p>
+	<a name="524"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>24<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Colony PCR adn electrophoresis of the electroporations</p>
+	<p style="font-size:18px">Minipreps and measure the concentration of 170, 159 and Tn7</p>
+	<p style="font-size:18px">Plate all the strains with pCdrA and the same strains withour pCdrA (fluorimeter and Congo Red)</p>
+	<p style="font-size:18px">Inocula of KT2442-T170/T159/T128, <i>lapG</i><sup>-</sup>-Tn7 and ΔbifA-T128</p>
+	<a name="525"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>25<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Inocula of the plates of yesterday</p>
+	<p style="font-size:18px">Colony PCR and electrophoresis of KT2442 <i>lapG</i><sup>-</sup>-Tn7/T133, ΔbifA-T128/T133 and KT2442-T133</p>
+	<p style="font-size:18px">Storage KT2442-T170/T159/T128, <i>lapG</i><sup>-</sup>-Tn7 and ΔbifA-T128 at -80ºC</p>
+	<a name="526"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>26<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Assay of the fluorimeter with all the constructions and ΔfleQ</p>
+	<p style="font-size:18px">Plate of Congo Red with KT2442, Tn7/T134/T136/T164/T165</p>
+	<p style="font-size:18px">Colony PCR and electrophoresis of KT2442-T133, KT2442 <i>lapG</i><sup>-</sup>-T133 and KT2442 ΔbifA-T133</p>
+	<a name="527"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>27<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Get the results of the fluorimeter</p>
+	<p style="font-size:18px">Let the plates with Congo Red 24 h more</p>
+	<p style="font-size:18px">Electrophoresis of the digestion of 164, cut a slice of gel that contains the fragment of interest and purify</p>
+	<a name="530"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>30<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Plate KT2442, KT2442-T134/T164, KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">A labmate got the plates with Congo Red and took a photo of ></p>
+	<a name="531"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>31<sup>th</sup> May</b></h3>
+	<p style="font-size:18px">Inocula of KT2442-T134/T164 and KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">Plate pMRB133</p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+  </div>
+ <div class="hr-divider"></div><a name="61"></a>
+	  <h2 style="text-align:center"><b>JUNE</b></h2>
+ <div id="june">
+ <div class="container">
+	<h3 style="color:#04B404"><b>1<sup>st</sup> June</b></h3>
+	<p style="font-size:18px">Dilutions and prepare curves of KT2442-T134/T164 and KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">Inocula of KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<a name="62"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>2<sup>nd</sup> June</b></h3>
+	<p style="font-size:18px">Dye and measure the plates of KT2442-T134/T164 and KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">Dilutions and prepare curves of KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">Inocula of 133</p>
+	<a name="63"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>3<sup>th</sup> June</b></h3>
+	<p style="font-size:18px">Dye and measure the plates of KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">Miniprep and measure the concentration of 133</p>
+	<a name="66"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>6<sup>th</sup> June</b></h3>
+	<p style="font-size:18px">Plate KT2442-Tn7/T134/T164 and KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">Diagnostic digestion and electrophoresis of 133</p>
+	<a name="67"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>7<sup>th</sup> June</b></h3>
+	<p style="font-size:18px">Inocula of KT2442-Tn7/T134/T164 and KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">Electroporation of KT2442, KT2442 <i>lapG</i><sup>-</sup> and ΔbifA with pMRB133</p>
+	<a name="68"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>8<sup>th</sup> June</b></h3>
+	<p style="font-size:18px">Dilutions and prepare curves of KT2442-T134/T164 and KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p>
+	<p style="font-size:18px">Colony PCR and electrophoresis of 133 electroporations</p>
+	<p style="font-size:18px">Inocula of KT2442/<i>lapG</i><sup>-</sup>/ΔbifA-T133</p>
+	<a name="69"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>9<sup>th</sup> June</b></h3>
+	<p style="font-size:18px">Dye and measure the plates of KT2442-T134/T164 and KT2442-Tn7/T128 and <i>lapG</i><sup>-</sup>-Tn7/T128</p><a name="610"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>10<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="613"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>13<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="614"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>14<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="615"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>15<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="616"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>16<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="617"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>17<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="620"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>20<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="621"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>21<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="622"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>22<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="624"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>24<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="627"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>27<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="628"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>28<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="629"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p><a name="630"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>30<sup>th</sup> June</b></h3>
+	<p style="font-size:18px"> </p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ </div>
+ <div class="hr-divider"></div><a name="71"></a>
+	  <h2 style="text-align:center"><b>JULY</b></h2>
+<div id="july">
+ <div class="container">
+	<h3 style="color:#04B404"><b>1<sup>st</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="74"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>4<sup>nd</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="75"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>5<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="76"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>6<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="77"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>7<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="78"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>8<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="711"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>11<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="712"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>12<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="713"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>13<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="714"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>14<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="715"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>15<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="718"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>18<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="719"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>19<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="720"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>20<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="721"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>21<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="722"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>22<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="725"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>25<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="726"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>26<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="727"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>27<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="728"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>28<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p><a name="729"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> July</b></h3>
+	<p style="font-size:18px"> </p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+  </div>
+ <div class="hr-divider"></div><a name="81"></a>
+	  <h2 style="text-align:center"><b>AUGUST</b></h2>
+ <div id="august">
+ <div class="container">
+	<h3 style="color:#04B404"><b>1<sup>st</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="82"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>2<sup>nd</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="83"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>3<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="84"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>4<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="85"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>5<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="88"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>8<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="89"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>9<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="810"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>10<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="811"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>11<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="812"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>12<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="815"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>15<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="816"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>16<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="817"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>17<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="818"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>18<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="819"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>19<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="822"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>22<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="823"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>23<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="824"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>24<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="825"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>25<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="826"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>26<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="829"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="830"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p><a name="831"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> August</b></h3>
+	<p style="font-size:18px"> </p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+  </div>
+ <div class="hr-divider"></div><a name="91"></a>
+	  <h2 style="text-align:center"><b>SEPTEMBER</b></h2>
+ <div id="september">
+ <div class="container">
+	<h3 style="color:#04B404"><b>1<sup>st</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="92"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>2<sup>nd</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="95"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>5<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="96"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>6<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="97"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>7<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="98"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>8<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="99"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>9<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="912"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>12<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="913"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>13<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="914"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>14<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="915"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>15<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="916"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>16<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="919"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>19<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="920"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>20<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="921"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>21<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="922"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>22<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="923"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>23<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="926"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+<div class="container">
+	<h3 style="color:#04B404"><b>26<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="927"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>27<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="928"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>28<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="929"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>29<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p><a name="930"></a>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ <div class="container">
+	<h3 style="color:#04B404"><b>30<sup>th</sup> September</b></h3>
+	<p style="font-size:18px"> </p>
+	<a href="#calendar" style="font-size:12px">Back to the calendar</a>
+ </div>
+ </div>
+ </body>
+<!--Footer
+==========================-->
+<footer>
+    <div class="container">
+      <div class="row">
+        <div class="span6"><br>
+        </div>
+        <div class="span6">
+            <div class="social pull-right">
+				<a href="https://www.youtube.com/channel/UCXUAle_ywH8F127dWBYrhgw" target="_blank" title="Youtube official page"><img src="https://static.igem.org/mediawiki/2016/a/ac/T--UPO-Sevilla--youtube.jpg" width="60" style="height:50px"</a>
+				<a href="https://www.facebook.com/profile.php?id=100006118518232&fref=ts" target="_blank" title="Facebook official page"><img src="https://static.igem.org/mediawiki/igem.org/thumb/8/8b/FaceBook-icon.png/120px-FaceBook-icon.png" width="60" height="60"></a>
+				<a href=https://twitter.com/iGEM_UPO target="_blank" title="Twitter official page"><img src="https://static.igem.org/mediawiki/igem.org/thumb/1/1a/Twitter_logo.png/120px-Twitter_logo.png" width="60" height="60"></a>
+				<a href="https://www.instagram.com/igemupo/" target="_blank" title="Instagram official page"><img src="https://static.igem.org/mediawiki/2016/thumb/f/f7/Instagram.jpeg/120px-Instagram.jpeg.png" width="60" height="60"></a>
+            </div>
+        </div>
+      </div>
+    </div>
+</footer>
+<!--/.Footer-->
+  </body>
 </html>

Difference between revisions of "Team:UPO-Sevilla/Notebook"

Revision as of 08:58, 11 October 2016

NOTEBOOK

JANUARY

1st January

4nd January

5th January

6th January

7th January

8th January

11th January

12th January

13th January

14th January

15th January

18th January

19th January

20th January

21th January

22th January

25th January

26th January

27th January

28th January

29th January

FEBRUARY

1st February

2nd February

3th February

4th February

5th February

8th February

9th February

10th February

11th February

12th February

15th February

16th February

17th February

18th February

19th February

22th February

23th February

24th February

25th February

26th February

29th February

MARCH

1st March

2nd March

3th March

4th March

7th March

8th March

9th March

10th March

11th March

14th March

15th March

16th March

17th March

18th March

21th March

22th March

23th March

24th March

25th March

28th March

29th March

30th March

31th March

APRIL

1st April

4nd April

5th April

6th April

7th April

8th April

11th April

12th April

1^st January

4^nd January

5^th January

6^th January

7^th January

8^th January

11^th January

12^th January

13^th January

14^th January

15^th January

18^th January

19^th January

20^th January

21^th January

22^th January

25^th January

26^th January

27^th January

28^th January

29^th January

1^st February

2^nd February

3^th February

4^th February

5^th February

8^th February

9^th February

10^th February

11^th February

12^th February

15^th February

16^th February

17^th February

18^th February

19^th February

22^th February

23^th February

24^th February

25^th February

26^th February

29^th February

1^st March

2^nd March

3^th March

4^th March

7^th March

8^th March

9^th March

10^th March

11^th March

14^th March

15^th March

16^th March

17^th March

18^th March

21^th March

22^th March

23^th March

24^th March

25^th March

28^th March

29^th March

30^th March

31^th March

1^st April

4^nd April

5^th April

6^th April

7^th April

8^th April

11^th April

12^th April

13^th April

14^th April

15^th April

18^th April

19^th April

20^th April

21^th April