Difference between revisions of "Team:Hong Kong HKUST/tourDebug"

 
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<body>
 
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<h1 class="title text-center" style="color:white;">
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<b>pTet Module</b>
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                        <br>       
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                        <br> 
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<div class="row">
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<p style="color: white; font-size: 15px;"> Hello guys welcome to the pTet Module!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?</p>
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<h7 style="color: white; font-size: 50px;"><b>pTet Module</b></h7>
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                                                <br>
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<p style="color: white; font-size: 15px">Hello guys welcome to the pTet Module! Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?
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<h2 class="text-center title"><b>Promoter, Repressor and Inducer Specification</b></h2>
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<div class="col-sm-3">
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                                        <img src="https://static.igem.org/mediawiki/2016/b/bf/T--Hong_Kong_HKUST--promoter_pTet.png" alt="pTet promoter" style="height: auto; width: 50%;">
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  </div>
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<div class="col-sm-9">
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<ul>
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                                          <li><b>Promoter name</b>: pTet (Tet repressible promoter)</li>
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                                          <li><b>Length</b>: 54 base pairs</li>
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                                          <li><b>Strength with</b>:
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                                              <ul>
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                                              <li>Weak RBS (BBa_B0032): 5.6 - 517.5 (<u>+</u>17) RPU</li>
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                                              <li>Medium RBS (BBa_B0030): 25.5 - 2323.1 (<u>+</u>259.5) RPU</li>
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                                              <li>Strong RBS (BBa_B0034): 39.6 - 4041.5 (<u>+</u>247.8) RPU</li>
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                                              </ul>
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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                                        <img src="https://static.igem.org/mediawiki/2016/d/dd/T--Hong_Kong_HKUST--Repressor_TetR.png" style="height: auto; width: 50%;">
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<div class="col-sm-9">
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<ul>
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                                          <li><b>Repressor name</b>: TetR (Tet repressor)</li>
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                                          <li><b>Part Length</b>: 685 base pairs</li>
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                                          <li><b>Structure</b>: Homodimer of alpha-helices</li>
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                                          <li><b>Binding affinity</b>: To pTet, very light, 0-1 µl/ml has been shown to cause a 5 order of magnitude change in fluorescent protein production</li>
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                                        <img src="https://static.igem.org/mediawiki/2016/6/60/T--Hong_Kong_HKUST--Inducer_aTc.png" style="height: auto; width: 50%;">
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  </div>
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<div class="col-sm-9">
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<ul>
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                                          <li><b>Inducer name</b>: ATc (Anhydrotetracycline)</li>
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                                          <li><b>Binding Affinity</b>: (to TetR) Ka ~ 109 M-1</li>
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                                          <li><b>Effective range</b>: 250 - 25000 ng/ml</li>
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<h3 class="widget-title">Contact</h3>
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<p>+234 23 9873237<br>
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<a href="mailto:#">some.email@somewhere.com</a><br>
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234 Hidden Pond Road, Ashland City, TN 37015
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<b>Mechanism of pTet - <i>TetR</i> - ATc Interaction</b>
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</h2>    
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?</p>
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2016/9/97/T--Hong_Kong_HKUST--pTet_TetR-ATc_Interaction.png">
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Latest revision as of 09:42, 11 October 2016

Home

pTet Module



Hello guys welcome to the pTet Module!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?

Promoter, Repressor and Inducer Specification


pTet promoter
  • Promoter name: pTet (Tet repressible promoter)
  • Length: 54 base pairs
  • Strength with:
    • Weak RBS (BBa_B0032): 5.6 - 517.5 (+17) RPU
    • Medium RBS (BBa_B0030): 25.5 - 2323.1 (+259.5) RPU
    • Strong RBS (BBa_B0034): 39.6 - 4041.5 (+247.8) RPU
  • Repressor name: TetR (Tet repressor)
  • Part Length: 685 base pairs
  • Structure: Homodimer of alpha-helices
  • Binding affinity: To pTet, very light, 0-1 µl/ml has been shown to cause a 5 order of magnitude change in fluorescent protein production
  • Inducer name: ATc (Anhydrotetracycline)
  • Binding Affinity: (to TetR) Ka ~ 109 M-1
  • Effective range: 250 - 25000 ng/ml

Mechanism of pTet - TetR - ATc Interaction