Difference between revisions of "Team:Hong Kong HKUST/tourDebug"

 
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<body>
 
<body>
  
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<section class="section jumbotron">
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<div class="content_wrapper_neo">
  
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<div class="header1">
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<h1 class="title text-center" style="color:white;">
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<div class="content_wrapper_neo">
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<b>pTet Module</b>
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<div class="row">
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</h1>
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                        <br>       
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                        <br> 
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<div class="row">
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<p style="color: white; font-size: 15px;"> Hello guys welcome to the pTet Module!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?</p>
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</div> <!-- /row  -->
  
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<h7 style="color: white; font-size: 50px;"><b>pTet Module</b></h7>
 
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                                                <br>
 
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                                                <br>
 
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<div class="widget-body">
 
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<p style="color: white; font-size: 15px">Hello guys welcome to the pTet Module! Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?
 
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</section>
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<div class="content_wrapper_neo">
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<h2 class="text-center title"><b>Promoter, Repressor and Inducer Specification</b></h2>
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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<br>
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<div class="header1">
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<div class="row">
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<div class="content_wrapper_neo">
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<div class="col-sm-3">
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<div class="row">
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                                        <img src="https://static.igem.org/mediawiki/2016/b/bf/T--Hong_Kong_HKUST--promoter_pTet.png" alt="pTet promoter" style="height: auto; width: 50%;">
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  </div>
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<div class="col-sm-9">
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<ul>
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                                          <li><b>Promoter name</b>: pTet (Tet repressible promoter)</li>
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                                          <li><b>Length</b>: 54 base pairs</li>
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                                          <li><b>Strength with</b>:
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                                              <ul>
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                                              <li>Weak RBS (BBa_B0032): 5.6 - 517.5 (<u>+</u>17) RPU</li>
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                                              <li>Medium RBS (BBa_B0030): 25.5 - 2323.1 (<u>+</u>259.5) RPU</li>
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                                              <li>Strong RBS (BBa_B0034): 39.6 - 4041.5 (<u>+</u>247.8) RPU</li>
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                                              </ul>
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</ul>
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</div>
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<h3 class="widget-title">Contact</h3>
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</section>
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<p>+234 23 9873237<br>
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<section class="section">
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<a href="mailto:#">some.email@somewhere.com</a><br>
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<br>
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<div class="row">
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234 Hidden Pond Road, Ashland City, TN 37015
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</p>
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                                        <img src="https://static.igem.org/mediawiki/2016/d/dd/T--Hong_Kong_HKUST--Repressor_TetR.png" style="height: auto; width: 50%;">
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<div class="col-sm-9">
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<ul>
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                                          <li><b>Repressor name</b>: TetR (Tet repressor)</li>
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                                          <li><b>Part Length</b>: 685 base pairs</li>
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                                          <li><b>Structure</b>: Homodimer of alpha-helices</li>
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                                          <li><b>Binding affinity</b>: To pTet, very light, 0-1 µl/ml has been shown to cause a 5 order of magnitude change in fluorescent protein production</li>
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</ul>
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</div>
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<h3 class="widget-title">Follow us</h3>
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<a href=""><i class="fa fa-github fa-2"></i></a>
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                                        <img src="https://static.igem.org/mediawiki/2016/6/60/T--Hong_Kong_HKUST--Inducer_aTc.png" style="height: auto; width: 50%;">
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  </div>
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<div class="col-sm-9">
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</div>
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<ul>
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</div>
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                                          <li><b>Inducer name</b>: ATc (Anhydrotetracycline)</li>
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                                          <li><b>Binding Affinity</b>: (to TetR) Ka ~ 109 M-1</li>
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                                          <li><b>Effective range</b>: 250 - 25000 ng/ml</li>
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</ul>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?</p>
 
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<p>Eius consequatur nihil quibusdam! Laborum, rerum, quis, inventore ipsa autem repellat provident assumenda labore soluta minima alias temporibus facere distinctio quas adipisci nam sunt explicabo officia tenetur at ea quos doloribus dolorum voluptate reprehenderit architecto sint libero illo et hic.</p>
 
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</footer>
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</section>
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<section class="section jumbotron">
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<div class="content_wrapper_neo">
  
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<h2 style="color:white;">
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<b>Mechanism of pTet - <i>TetR</i> - ATc Interaction</b>
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</h2>     
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                        <br> 
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2016/9/97/T--Hong_Kong_HKUST--pTet_TetR-ATc_Interaction.png">
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</div>
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</section>
 
<!--
 
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<script>
 
<script>

Latest revision as of 09:42, 11 October 2016

Home

pTet Module



Hello guys welcome to the pTet Module!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?

Promoter, Repressor and Inducer Specification


pTet promoter
  • Promoter name: pTet (Tet repressible promoter)
  • Length: 54 base pairs
  • Strength with:
    • Weak RBS (BBa_B0032): 5.6 - 517.5 (+17) RPU
    • Medium RBS (BBa_B0030): 25.5 - 2323.1 (+259.5) RPU
    • Strong RBS (BBa_B0034): 39.6 - 4041.5 (+247.8) RPU
  • Repressor name: TetR (Tet repressor)
  • Part Length: 685 base pairs
  • Structure: Homodimer of alpha-helices
  • Binding affinity: To pTet, very light, 0-1 µl/ml has been shown to cause a 5 order of magnitude change in fluorescent protein production
  • Inducer name: ATc (Anhydrotetracycline)
  • Binding Affinity: (to TetR) Ka ~ 109 M-1
  • Effective range: 250 - 25000 ng/ml

Mechanism of pTet - TetR - ATc Interaction