Difference between revisions of "Team:Hong Kong HKUST/tourDebug"

 
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<h2 class="text-center title">Frequently Asked Questions</h2>
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<h2 class="text-center title"><b>Promoter, Repressor and Inducer Specification</b></h2>
 
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<h4><b>Which code editor would you recommend?</b></h4>
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                                        <img src="https://static.igem.org/mediawiki/2016/b/bf/T--Hong_Kong_HKUST--promoter_pTet.png" alt="pTet promoter" style="height: auto; width: 50%;">
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<p>I'd highly recommend you <a href="http://www.sublimetext.com/">Sublime Text</a> - a free to try text editor which I'm using daily. Awesome tool!</p>
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                                          <li><b>Promoter name</b>: pTet (Tet repressible promoter)</li>
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                                          <li><b>Length</b>: 54 base pairs</li>
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                                          <li><b>Strength with</b>:
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                                              <ul>
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                                              <li>Weak RBS (BBa_B0032): 5.6 - 517.5 (<u>+</u>17) RPU</li>
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                                              <li>Medium RBS (BBa_B0030): 25.5 - 2323.1 (<u>+</u>259.5) RPU</li>
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                                              <li>Strong RBS (BBa_B0034): 39.6 - 4041.5 (<u>+</u>247.8) RPU</li>
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<h4><b>Nice header. Where do I find more images like that one?</b></h4>
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Well, there are thousands of stock art galleries, but personally,
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I prefer to use photos from these sites: <a href="http://unsplash.com">Unsplash.com</a>
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and <a href="http://www.flickr.com/creativecommons/by-2.0/tags/">Flickr - Creative Commons</a></p>
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                                        <img src="https://static.igem.org/mediawiki/2016/d/dd/T--Hong_Kong_HKUST--Repressor_TetR.png" style="height: auto; width: 50%;">
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                                          <li><b>Repressor name</b>: TetR (Tet repressor)</li>
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                                          <li><b>Part Length</b>: 685 base pairs</li>
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                                          <li><b>Structure</b>: Homodimer of alpha-helices</li>
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                                          <li><b>Binding affinity</b>: To pTet, very light, 0-1 µl/ml has been shown to cause a 5 order of magnitude change in fluorescent protein production</li>
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<h4><b>Can I use it to build a site for my client?</b></h4>
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Yes, you can. You may use this template for any purpose, just don't forget about the <a href="http://creativecommons.org/licenses/by/3.0/">license</a>,
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which says: "You must give appropriate credit", i.e. you must provide the name of the creator and a link to the original template in your work.
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                                        <img src="https://static.igem.org/mediawiki/2016/6/60/T--Hong_Kong_HKUST--Inducer_aTc.png" style="height: auto; width: 50%;">
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<h4><b>Can you customize this template for me?</b></h4>
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<p>Yes, I can. Please drop me a line to sergey-at-pozhilov.com and describe your needs in details. Please note, my services are not cheap.</p>
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                                          <li><b>Inducer name</b>: ATc (Anhydrotetracycline)</li>
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                                          <li><b>Binding Affinity</b>: (to TetR) Ka ~ 109 M-1</li>
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                                          <li><b>Effective range</b>: 250 - 25000 ng/ml</li>
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<h2 style="color:white;">
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<b>Mechanism of pTet - <i>TetR</i> - ATc Interaction</b>
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                        <br> 
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<img class="img-responsive" src="https://static.igem.org/mediawiki/2016/9/97/T--Hong_Kong_HKUST--pTet_TetR-ATc_Interaction.png">
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Latest revision as of 09:42, 11 October 2016

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pTet Module



Hello guys welcome to the pTet Module!Lorem ipsum dolor sit amet, consectetur adipisicing elit. Excepturi, dolores, quibusdam architecto voluptatem amet fugiat nesciunt placeat provident cumque accusamus itaque voluptate modi quidem dolore optio velit hic iusto vero praesentium repellat commodi ad id expedita cupiditate repellendus possimus unde?

Promoter, Repressor and Inducer Specification


pTet promoter
  • Promoter name: pTet (Tet repressible promoter)
  • Length: 54 base pairs
  • Strength with:
    • Weak RBS (BBa_B0032): 5.6 - 517.5 (+17) RPU
    • Medium RBS (BBa_B0030): 25.5 - 2323.1 (+259.5) RPU
    • Strong RBS (BBa_B0034): 39.6 - 4041.5 (+247.8) RPU
  • Repressor name: TetR (Tet repressor)
  • Part Length: 685 base pairs
  • Structure: Homodimer of alpha-helices
  • Binding affinity: To pTet, very light, 0-1 µl/ml has been shown to cause a 5 order of magnitude change in fluorescent protein production
  • Inducer name: ATc (Anhydrotetracycline)
  • Binding Affinity: (to TetR) Ka ~ 109 M-1
  • Effective range: 250 - 25000 ng/ml

Mechanism of pTet - TetR - ATc Interaction