Safety

1.General Lab Safety Rules

All team members follow the generallab safety rules and receive safety training before experiment. Our bench workfollows the basic safety rules as following:

            1.No foodand drink in the lab.

            2.Wearrubber gloves and lab coat in all experiments.

            3.Bio-safetycabinet is required when using bacteria.

            4.UsingUV-light to sterilize the lab every week.

            5.Turn offfire, electric heaters when leaving.

            6.Liquidwaste and bioorganic waster must be sterilized

2.Mosquitoes Feeding Safety Rules

Our mosquitoes feeding experimentdepends on the KeyLaboratory of Biopesticide and Chemical Biology which is one of the bestmosquito research labs in China. To keep experimenters and public safety,experimenters should follow the additional safety rules as following:

            1.Everyone should register before entering the feedingrooms.

2.Staffmust practice protective measure before experiment.

            2.The feeding rooms must be sterilized if any mosquitoesescaped. The room will not be accessible until the 15-days disinfection isover.

            3.The feeding areas of Culex fatigans, Aedes aegyptiand White albopictus must beseparated and closed to ensure safety.

            4. The feeding areas of larvae and adults must beseparated.

            5. Seeking medical advice is required when stuff isbited.

3. Safety Rules of Chlamydomonas reinhardtii

Chlamydomonas reinhardtiiis a type of model organism in the genetic engineering filed. It has beenproved that Chlamydomonas reinhardtii issafe for research. Meanwhile, we used the CC503 and FA479 strains which have notoxicity to any organisms.

Theultimate goal of our project is to use Chlamydomonasreinhardtii which can produce mosquito-larvicidal proteins in the naturalenvironment to control mosquitoes. Considering the potential possibility ofalga bloom, we decided to use CC503 strain which is cell wall deficient. Thecell wall deficient strain can not survive for a long time. Because of it, Chlamydomonas reinhardtii amostly cannot become the dominant population in the natural environment.

What’smore, BNU-CHINA team helped us to build the growth model of Chlamydomonas reinhardtii in the naturalmodel. Based on the experiment of growth under the different conditions, we canpredict the amount of Chlamydomonasreinhardtii after releasing in the natural environment.

Inthe future, we plan to add non-functional gene fragment tag to expressionvector. We synthesis a particular DNA sequence which has no homology sequencein Chlamydomonas reinhardtii. Meanwhile,we design the corresponding primer about this sequence. The department ofenvironment or any biology department can make use of PCR to detect the leak ofChlamydomonas reinhardtii.