Difference between revisions of "Team:Manchester/Notebook"
| Line 41: | Line 41: | ||
color: orange; | color: orange; | ||
padding-bottom: 20px; | padding-bottom: 20px; | ||
| + | } | ||
| + | |||
| + | .full1{ | ||
| + | width: 100%; | ||
} | } | ||
| Line 105: | Line 109: | ||
<li>Restriction enzyme digest of constitutive promoters and control(pUC19) for validation with EcoRI and PstI</li> | <li>Restriction enzyme digest of constitutive promoters and control(pUC19) for validation with EcoRI and PstI</li> | ||
</ul> | </ul> | ||
| + | |||
| + | <img class="full1" src="https://static.igem.org/mediawiki/2016/c/c9/T--Manchester--notebook_mech2_1.png" alt="figure 1" /> | ||
| + | <img class="full1" src="https://static.igem.org/mediawiki/2016/5/5e/T--Manchester--notebook_mech2_2.png" alt="figure 1" /> | ||
| + | |||
</div> | </div> | ||
Revision as of 21:23, 13 October 2016
Notebook
Inducible Gene Switch
- Re-suspended DNA (constitutive promoters) from iGEM Distribution kit and transformed them into DH5α strain.
- Growth was found on the negative control plates so re-transformed DNA
- Inoculated of transformed cells.
Pilot Experiment
- Made stocks of our reagents Glucose Stock for 0.005 g/mL, Glucose Oxidase Stock for 0.0025 g/mL, HRP Stock for 250 μL/mL, ABTS for 0.0025 g/mL
Inducible Gene Switch
- Performed miniprep for overnight cultures
- Prepared fresh batch of DH5α chemical competent cells
- Restriction enzyme digest of constitutive promoters and control(pUC19) for validation with EcoRI and PstI
Pilot Experiment
- Still awaiting the arrival of the BMG spectrophotometer
Inducible Gene Switch
- Prepared DH5α chemical competent cells
- Prepared Chloramphenicol antibiotic stock
- Restreaked alcA 1 (BBa_K678001) and spispink(BBa_K1033923 pink chromoprotein)
