Difference between revisions of "Team:Bielefeld-CeBiTec/Collaborations"

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<h1>Collaborations</h1>
 
<h1>Collaborations</h1>
 
<center><img src="http://www.uleth.ca/research/sites/uleth.ca.research/themes/flora_evo/logo.png" /></center>
 
<center><img src="http://www.uleth.ca/research/sites/uleth.ca.research/themes/flora_evo/logo.png" /></center>
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We were thrilled after realising that this year’s project from <a href="https://2016.igem.org/Team:Lethbridge">Lethbridge University</a> was similar to our project. They also wanted to screen a library of nanobodies using a bacterial two-hybrid system. After initiating the contact we had several skype meetings (<a href=”#”>see here for a complete skype diary</a>) where we exchanged ideas and talked about each other’s approaches towards the experiments. After a intensive discussion about bacterial two-hybrid systems, we realized that Lethbridge has no positive controls for their two-hybrid system. Therefore we sent them our positive controls: <a href=”#”>the HA4-Abl SH2 binding pair including the different mutants of HA4</a>. In this way,we hope to give iGEM Lethbridge the tools to validate their two-hybrid system.
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We were thrilled after realising that this year’s project from <a href="https://2016.igem.org/Team:Lethbridge">Lethbridge University</a> was similar to our project. They also wanted to screen a library of nanobodies using a bacterial two-hybrid system. After initiating the contact we had several skype meetings (<a href=”https://2016.igem.org/Team:Bielefeld-CeBiTec/Collaborations/Lethbridge”>see here for a complete skype diary</a>) where we exchanged ideas and talked about each other’s approaches towards the experiments. After a intensive discussion about bacterial two-hybrid systems, we realized that Lethbridge has no positive controls for their two-hybrid system. Therefore we sent them our positive controls: <a href=”#”>the HA4-Abl SH2 binding pair including the different mutants of HA4</a>. In this way,we hope to give iGEM Lethbridge the tools to validate their two-hybrid system.
 
In exchange, Lethbridge provided us with the NS1 peptide from influenza A virus. We wanted to try this peptide as a potential target for our Evobodies. Therefore we could generate Evobodies against the important pathogen influenza A as well.     
 
In exchange, Lethbridge provided us with the NS1 peptide from influenza A virus. We wanted to try this peptide as a potential target for our Evobodies. Therefore we could generate Evobodies against the important pathogen influenza A as well.     
 
   
 
   

Revision as of 02:04, 14 October 2016




Collaborations

We were thrilled after realising that this year’s project from Lethbridge University was similar to our project. They also wanted to screen a library of nanobodies using a bacterial two-hybrid system. After initiating the contact we had several skype meetings (see here for a complete skype diary) where we exchanged ideas and talked about each other’s approaches towards the experiments. After a intensive discussion about bacterial two-hybrid systems, we realized that Lethbridge has no positive controls for their two-hybrid system. Therefore we sent them our positive controls: the HA4-Abl SH2 binding pair including the different mutants of HA4. In this way,we hope to give iGEM Lethbridge the tools to validate their two-hybrid system. In exchange, Lethbridge provided us with the NS1 peptide from influenza A virus. We wanted to try this peptide as a potential target for our Evobodies. Therefore we could generate Evobodies against the important pathogen influenza A as well.